Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae

Dong Liang-feng; M. J. Merrett; Wu Chao-yuan

doi:10.1007/BF02842599

Your Location：

Home >

Browse articles >

Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae

Updated：2023-05-16

- Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae
- Journal of Oceanology and Limnology Vol. 17, Issue 3, Pages: 219-232(1999)
- Affiliations：
  
  1. Department of Biology John Tabor Laboratory University of Essex
  2. School of Biological Sciences University of Wales Swansea
  3. Department of Marine Botany Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/BF02842599
  CLC：
- Published：1999
- Accepted：
Scan QR Code
Liang-feng, D., Merrett, M.J. & Chao-yuan, W. Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae., Chin. J. Ocean. Limnol. 17, 219–232 (1999). https://doi.org/10.1007/BF02842599
DOI：

Liang-feng, D., Merrett, M.J. & Chao-yuan, W. Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae., Chin. J. Ocean. Limnol. 17, 219–232 (1999). https://doi.org/10.1007/BF02842599 DOI：

摘要

Abstract

Inorganic carbon utilization in the non-calcifying marine microalgae

Nannochloropsis oculata

Phaeodactylum tricornutum andPorphyridium purpureum was compared with high- and low-calcifying strains ofEmiliania huxleyi grown in artificial seawater medium aerated with either air (0.03% V/V CO2) or CO2-free air. For high-calcifying strain ofE. oculata andP. tricornutem

similar growth patterns were observed in air-and CO2-free air-grown cultures.P. purpureum showed a less final cell density in CO2-free air than in air-grown culture. However

low-calcifying strain ofE. huxleyi was able to grow only in air-grown culture

but not in CO2-free air-grown culture. Measurements of alkalinity

concentration of dissolved inorganic carbon (DIC) and free CO2 showed different patterns of DIC utilization. WithN. oculata

P. tricornutum andP. purpureum the pattern of DIC utilization was characterized by an increase of pH and a decrease of DIC but a constant alkalinity in the cultures aerated with air or CO2-free air

suggesting that bicarbonate utilization was concomitant with an efflux of OH−. Both alkalinity and pH were maintained rather constant in air-grown culture of low-calcifying strain ofE. huxleyi

suggesting that diffusive entry of CO2 could meet the requirement of DIC for its photosynthesis and growth. High-calcifying strain ofE. huxleyi

however

showed a pattern of decrease of alkalinity and DIC but an almost constant pH

indicating that bicarbonate was the major form of inorganic carbon utilised by this organism and bicarbonate uptake is unlikely to be accompanied by an efflux of OH−. The final pH values reached byN. oculata

P. tricornutum andP. purpureum in a closed system were 10.75

10.60 and 9.85 respectively

showing that bicarbonate utilisation is concomitant with an efflux of OH−. While the final pH of 8.4 in high-calcifyingE. huxleyi suggests that bicarbonate utilization was not accompanied by an efflux of OH−.

关键词

Keywords

references

Allen, E. D., Spence D. H. N., 1981. The differential ability of aquatic plants to utilize the inorganic supply in freshwaters.New Phytologist 87: 269–283.

Axelsson, L., Unsitalo, J., 1988. Carbon acquisition strategies for marine macroalgae I. Utilization of proton exchanges visualized during photosynthesis in a closed system.Marine Biology 97: 295–300.

Brown C. W., Yoder, J. A., 1994.Coccolithophorid blooms in the global ocean.Journal of Geophysical Research 99(C): 7467–7482.

Buitenhuis, E., Bleijswijk, J. Van, Bakker, D. et al., 1996. Trend in inorganic and organic carbon in a bloom ofEmiliania huxleyi in the North Sea.Mar. Ecol. Prog. Series 143: 271–282.

Burns, B. D., Beardall, J., 1987. Utilization of inorganic carbon by marine microalgae.Journal of Experimental Marine Biology and Ecology 107: 75–86.

Butler, J. W., 1982. Carbon dioxide equilibra and their applications. Addison-Wesley Publishing Company. p. 20–21.

Colman, B., Gehl, K. A., 1983. Physiological characteristics of photosynthesis inPorphyridium cruentum: Evidence for bicarbonate transport in an unicellular red alga.Journal of Experimental Botany 37: 977–984.

Cook, C. M., Lanaras, T., Colman, B., 1986. Evidence for bicarbonate transport in species of red and brown macrophytic marine algae.Journal of Experimental Botany 37: 977–984.

Dixon, G. K., Merrett, M. J., 1988. Bicarbonate utilization by the marine diatomPhaeadactylum tricornutum.Bohlin New Phytologist 109: 45–51.

Dong, L. F., 1992. Inorganic carbon acqusition in seaweeds. PhD thesis, University of London.

Dong, L. F., Nimer, N. A., Okus, E., Merrett, M. J., 1993. Dissolved inorganic carbon utilization in relation to calcite production inEmiliania huxleyi (Lohmann) Kamptner.New Phytologist 123: 679–684.

Dong, L. F., Thake, B., Heathcote, B., 1991. The role of carbonic anhydrase in carbon acquisition in three species of seaweeds. Oebalia XVII 1 Suppl. p. 49–63.

Goyet, C., Poisson, A., 1989. New determination of carbonic acid dissociation constants in sea water as a function of temperature and salinity.Deep Sea Research 36: 1635–1654.

Holligan, P. M., 1993. A biogeochemical study of the coccolithophore,Emiliania huxleyi, in the North Atlantic.Global Biogeochem. Cycles 7: 879–900.

Holligan, P. M., Groom, S. B., 1986. Phytoplankton distributions along the shelf break.Proceedings of the Royal Society of Edinburgh 88: 239–63.

Ibelings, B. W., Maberly, S. C., 1998. Photoinhibition and the availability of inorganic carbon restrict photosynthesis by surface blooms of cyanobacteria.Limnol. Oceanogr. 43: 408–419.

Kerby, N. W., Raven, J. A., 1985. Transport and fixation of inorganic carbon by marine algae.Advance in Botanical Research 11: 71–123.

Lohamann, H., 1908. Uber die Beziehungen zwischen den pelagisch Ablagerungen und dem plankton des meers.International Revve der Gasamten Hydrobiologie und Hydrographie 1: 309–323.

Lucas, W. J., 1979. Alkaline band formation in Chara corallina due to OH− efflux or H+ influx?Plant Physiology 63: 248–254.

Maberly, S. C., 1990. Exogenous sources of inorganic carbon for photosynthesis by marine macroalgae.Journal of Phycology 26: 439–449.

Maberly, S. C., Spence, D. H. N., 1983. Photosynthetic inorganic carbon use by freshwater plants.Journal of Ecology 71: 705–724.

Mann, K. H., Lazier, J. R. N., 1991. Dynamics of Marine Ecosystems. Blackwell Scientific Publications p. 384–419.

Meldrum, N. U., Roughton, F. J. W., 1933. Carbonic anhydrase: its preparation and properties.Journal of Physiology 80: 113–142.

Merrett, M. J., 1991. Inorganic carbon transport in some marine microalgal species.Canadian Journal of Botany 69: 1032–1039.

Miller, A. G., Colman, B., 1980. Evidence for bicarbonate transport by the blue-green alga (cyanobacterium)Coccochloris peniocystis.Plant Physiology 65: 397–402.

Miyachi, S., Tsuzuki, M., Avramova, S. T., 1983. Utilization model of inorganic carbon for photosynthesis in various species ofChlorella.Plant Cell Physiology 24: 441–451.

Miyachi, S., Tsuzuki, M., Yagawa, Y., 1985. Carbonic anhydrase in various microalgae.In: Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms. Eds Lucas, W. J. and Berry, J. A. American Society of Plant Physiology, Rockville, Maryland p. 145–154.

Moroney, J. V., Husic, H. D., Tolbert, N. E., 1985. Effect of carbonic anhydrase inhibitors on inorganic carbon accumulation byChlamydononas reinhardtii.Plant Physiology 79: 177–183.

Nimer, N. A., Merrett, M. J., 1992. Calcification and utilization of inorganic carbon by the coccolithophoridEmiliania huxleyi Lohmann.New Phytologist 121: 173–177.

Nimer, N. A., Merrett, M. J., 1993. Calcification rate inEmiliania huxleyi in response to light, nitrate and inorganic carbon availability.New Phytologist 123: 673–677.

Paasche, E., 1964. A tracer study of the inorganic carbon uptake during coccolith formation and photosynthesis in coccolithophoridCoccolith huxleyi.Physiologia Plantarum (Supplement)3: 1–82.

Paasche, E., 1992 The physiology ofEmiliania huxleyi. Abstract of the third GEM workshop. p. 10–20.

Paasche, E., Brubak, S., 1994. Enhanced calcification in the coccolithophridEmiliania huxleyi under phosphorus limitation.Phycologia 33: 324–330.

Parsons, T., Maita, Y. R., Lalli, C. M., 1989. A manual of chemical and Biological Methods for Sea—Water analysis. Pergamon Press. p. 141–148.

Patel, B. N., Merrett, M. J., 1986. Inorganic carbon uptake by the marine diatomPhaeodactylum tricornutum.Planta 169: 222–227.

Purdie, D. A., Finch, M. S., 1994. Impact of acoccolithophorid on dissolved CO2 in seawater enclosures in a Norwegian fjord.Sarsia 79: 379–387.

Riley, J. P., Chester, R., 1971. Introduction to Marine Chemistry. London, New York, Academic Press, p. 121–151.

Bobertson, J. E., 1994. The impact of a coccolithophore bloom on oceanic carbon uptake in the northeast Atlantic during summer 1991.Deep—Sea Research 41: 297–314.

Sikes, C. S., Wheeler, A. P., 1982. Carbonic anhydrase and carbon fixation incoccolithophorids.Journal of Phycology 18: 123–126.

Sikes, C. S., Roer, R. D., Wilbur, K. M., 1980. Photosynthesis and coccolith formation: inorganic carbon sources and net inorganic reaction of deposition.Limnology and Oceanography 25: 248–261.

Smith, R. G., Bidwell, R. G. S., 1989. Mechanisms of photosynthetic carbon dioxide uptake by the red macroalgaChondrus crispus.Plant Physiology 89: 93–99.

Spence, D. H. N., Maberly, S. C., 1985. Occurrence and ecological importance of HCO3 − use among aquatic higher plants.In: Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms. Eds Lucas, W. J. and Berry, J. A. American Society of Plant Physiology, Rockville, Maryland, p. 125–143.

Surif, M. B., Raven, J. A., 1989. Exogenous inorganic carbon sources for photosynthesis in seawater by members of the Fucales and Laminariales (Phaeophyta): ecological and taxonomic implications.Oecologia 78: 91–105.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Hydrochemical characteristics and the ecological effect of algal carbonic anhydrase in carbon cycle in the Taiyuan section of Fenhe River

Methodology for studies of carbon dynamics between phytoplankton primary production and heterotrophic bacterial secondary production

The carbon cycle in Daya Bay

Response of cyanobacterial carbon concentrating system to light intensity: a simulated analysis

Analysis Procedure and Numerical Simulation of Failure Probability of Turbine Disk Caused by Defects

Related Author

Jing YANG

Xin LI

Shulian XIE

Jia FENG

Lin Wanlian

Jurgen Overbeck

Han Wuying

Lin Hongying

Related Institution

Shanxi Key Laboratory of Yuncheng Salt Lake Ecological Protection and Resource Utilization, College of Life Sciences, Yuncheng University

School of Life Science, Shanxi University

Institute of Hydrobiology

Dept. of Microbial Ecology Max-Planck

South China Sea Institute of Oceanology

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰