Metabolomics analysis of the mechanism underlying male caste differentiation in the giant freshwater prawn <italic style="font-style: italic">Macrobrachium rosenbergii</italic>

Ziqi ZHOU; Guanghua HUANG; Miuying CAI; Shunkai YIN; Bobo LI; Xuefang LANG; Jiongying YU; Qiongying TANG; Quanxin GAO; Shaokui YI

doi:10.1007/s00343-026-5386-z

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Metabolomics analysis of the mechanism underlying male caste differentiation in the giant freshwater prawn Macrobrachium rosenbergii

Updated：2026-09-28

- Metabolomics analysis of the mechanism underlying male caste differentiation in the giant freshwater prawn Macrobrachium rosenbergii
- Metabolomics analysis of the mechanism underlying male caste differentiation in the giant freshwater prawn Macrobrachium rosenbergii
- 海洋湖沼学报(英文) 2026年页码：1-18
- Affiliations：
  
  1.College of Life Science, Huzhou Normal University, Huzhou 313000, China
  2.Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China
  3.Jiangsu Shufeng Prawn Breeding Co., Ltd., Gaoyou 225654, China
  4.Zhejiang Guoliang Giant Prawn Genetic Breedig Co., Ltd., Huzhou 313000, China
  5.Extension and Service Center for Agricultural Technology of Wuxing District, Huzhou 313099, China
- Author bio：
  
  gaoqx2008@163.com
  yishaokui@foxmail.com
- Funds：
  
  the National Natural Science Foundation of China(32273121);the R&D Project from Zhejiang Provincial Department of Agriculture and Rural Affairs(ZAT-2024-9);the earmarked fund for Modern Agro-industry Technology Research System(CARS-48)
- DOI：10.1007/s00343-026-5386-z
  中图分类号：
- 收稿：2025-10-04，
  
  网络首发：2026-09-28，
- Accepted：
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Ziqi ZHOU, Guanghua HUANG, Miuying CAI, 等. Metabolomics analysis of the mechanism underlying male caste differentiation in the giant freshwater prawn Macrobrachium rosenbergii[J/OL]. 海洋湖沼学报(英文), 2026,1-18. DOI: 10.1007/s00343-026-5386-z.

ZHOU Ziqi,HUANG Guanghua,CAI Miuying,et al.Metabolomics analysis of the mechanism underlying male caste differentiation in the giant freshwater prawn Macrobrachium rosenbergii[J].Journal of Oceanology and Limnology,
Ziqi ZHOU, Guanghua HUANG, Miuying CAI, 等. Metabolomics analysis of the mechanism underlying male caste differentiation in the giant freshwater prawn Macrobrachium rosenbergii[J/OL]. 海洋湖沼学报(英文), 2026,1-18. DOI: 10.1007/s00343-026-5386-z. DOI：

ZHOU Ziqi,HUANG Guanghua,CAI Miuying,et al.Metabolomics analysis of the mechanism underlying male caste differentiation in the giant freshwater prawn Macrobrachium rosenbergii[J].Journal of Oceanology and Limnology, DOI：10.1007/s00343-026-5386-z.

摘要

Abstract

The giant freshwater prawn (

Macrobrachium

rosenbergii

) is a globally important species in aquaculture

prized for it

s delicious

tender meat and favored by consumers. Research has revealed a distinct hierarchical differentiation phenomenon among male

rosenbergii

classifying them into three morphotypes: blue claw (BC)

orange claw (OC)

and small male (SM). This differentiation has become a key factor constraining industry development. This study collected serum samples from male prawns during critical stages of this differentiation (at 100-

110-

and 120-d-old). Utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with untargeted metabolomics analysis

differential metabolites were identified through principal component analysis and database comparison. Results show that differential metabolites such as caffeoylcholine

glucosamine

and phosphate were significantly enriched in amino acid metabolism

ABC transporter

and nucleotide metabolism pathways

indicating that these metabolites and pathways may be involved in regulating the hierarchical differentiation of male

rosenbergii

. Further integration of 16S rDNA sequencing

transcriptome sequencing

and phenotypic data enabled the construction of an “Intestinal Microbiota- Phenotypic Traits-Metabolites” correlation model. The study found significant associations between intestinal bacteria (

Lactococcus

spp.

Achromobacter

spp.)

the host trypsin gene

and various differential metabolites. This suggests that these host genes and intestinal microbiota play important roles in the phenotypic differentiation and nutrient metabolism processes of

rosenbergii

. This research aims to elucidate the metabolic mechanisms underlying phenotypic differentiation in male

rosenbergii

through metabolomics approaches. By integrating 16S rDNA and transcriptome sequencing

it clarifies the regulatory roles of key metabolites

gut microbiota

and host genes in this process

providing an important

theoretical basis for the selective breeding and efficient aquaculture of

rosenbergii

关键词

Keywords

references

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Metabolomics analysis of the mechanism underlying male caste differentiation in the giant freshwater prawn Macrobrachium rosenbergii

DOI：10.1007/s00343-026-5386-z

摘要

Abstract

关键词

Keywords

references