New molecular sequences illuminate two new <italic style="font-style: italic">Anthophysa</italic> species (Chrysophyceae, Ochromonadales) from China: integrating phylogenetic and morphological evidence

Yalu AN; Junxue HAO; Fangru NAN; Qi LIU; Xudong LIU; Shulian XIE; Jia FENG

doi:10.1007/s00343-026-5287-1

Your Location：

Home >

Browse articles >

New molecular sequences illuminate two new Anthophysa species (Chrysophyceae, Ochromonadales) from China: integrating phylogenetic and morphological evidence

Updated：2026-05-07

- New molecular sequences illuminate two new Anthophysa species (Chrysophyceae, Ochromonadales) from China: integrating phylogenetic and morphological evidence
- New molecular sequences illuminate two new Anthophysa species (Chrysophyceae, Ochromonadales) from China: integrating phylogenetic and morphological evidence
- 海洋湖沼学报(英文) 2026年页码：1-13
- Affiliations：
  
  School of Life Sciences, Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University, Taiyuan 030006, China
- Author bio：
  
  fengj@sxu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(32270220;U22A20445 to Jia FENG);the Research Project Supported by Shanxi Scholarship Council of China(2024-007);the Sanjin Talent Innovation Teams in Natural Sciences and Engineering Technology to Jia FENG
- DOI：10.1007/s00343-026-5287-1
  中图分类号：
- 收稿：2025-08-20，
  
  修回：2025-10-28，
  
  网络首发：2026-05-07，
- Accepted：
Scan QR Code
Yalu AN, Junxue HAO, Fangru NAN, 等. New molecular sequences illuminate two new Anthophysa species (Chrysophyceae, Ochromonadales) from China: integrating phylogenetic and morphological evidence[J/OL]. 海洋湖沼学报(英文), 2026,1-13.

AN Yalu,HAO Junxue,NAN Fangru,et al.New molecular sequences illuminate two new Anthophysa species (Chrysophyceae, Ochromonadales) from China: integrating phylogenetic and morphological evidence[J].Journal of Oceanology and Limnology,
Yalu AN, Junxue HAO, Fangru NAN, 等. New molecular sequences illuminate two new Anthophysa species (Chrysophyceae, Ochromonadales) from China: integrating phylogenetic and morphological evidence[J/OL]. 海洋湖沼学报(英文), 2026,1-13. DOI： 10.1007/s00343-026-5287-1.

AN Yalu,HAO Junxue,NAN Fangru,et al.New molecular sequences illuminate two new Anthophysa species (Chrysophyceae, Ochromonadales) from China: integrating phylogenetic and morphological evidence[J].Journal of Oceanology and Limnology, DOI：.

摘要

Abstract

The taxonomic basis of the g

enus

Anthophysa

has remained unclear since its establishment in 1822. Only one partial LSU rDNA sequence is available to date

and its taxonomic position in the Chrysophyceae remains largely unknown. We combined morphological characteristics with newly acquired molecular evidence

including the nuclear SSU

ITS

and complete LSU rDNA

and identified two strains of the genus

Anthophysa

collected from Xizang and Shanghai

China. Distinguishing species within this genus based solely on morphological characteristics was found quite challenging

as the two strains were morphologically extremely similar. The phylogenetic trees constructed based on the LSU single gene and the SSU

ITS

and LSU multiple genes supported the identification of the specimen from Xizang as

Anthophysa

baimaiquensis

sp. nov. and that from Shanghai as

shanghaiensis

sp. nov. Moreover

the taxonomic position of the genus

Anthophysa

within the class Chrysophyceae and its topological position on the phylogenetic tree were clarified. We identified the origin of

Anthophysa

through Bayesian relaxed-clock analysis

thereby providing an important temporal basis for reconstructing the evolutionary history of

Anthophysa

关键词

Keywords

references

Ali A B , De Baere R , Van Der Auwera G et al . 2001 . Phylogenetic relationships among algae based on complete large-subunit rRNA sequences . International Journal of Systematic and Evolutionary Microbiology , 51 ( 3 ): 737 - 749 , https://doi.org/10.1099/00207713-51-3-737 https://doi.org/10.1099/00207713-51-3-737 .

An Y L , Hao J X , Nan F R et al . 2025 . New records of the colonial chrysophyte Urostipulosphaera articulata (Chrysophyceae, Ochromonadales) in China . Biodiversity Data Journal , 13 : e 150900 , https://doi.org/10.3897/BDJ.13.e150900 https://doi.org/10.3897/BDJ.13.e150900 .

Andersen R A , Graf L , Malakhov Y et al . 2017 . Rediscovery of the Ochromonas type species Ochromonas triangulata (Chrysophyceae) from its type locality (Lake Veysove, Donetsk region, Ukraine) . Phycologia , 56 ( 6 ): 591 - 604 , https://doi.org/10.2216/17-15.1 https://doi.org/10.2216/17-15.1 .

Andersen R A , Woelkerling W J . 2017 . Lectotype designation for Anthophysa vegetans (O.F.Müller) F.Stein (Ochromonadales, Chrysophyceae) . Notulae algarum , 22 : 1 - 5 .

Beisser D , Graupner N , Bock C et al . 2017 . Comprehensive transcriptome analysis provides new insights into nutritional strategies and phylogenetic relationships of chrysophytes . PeerJ , 5 : e 2832 , https://doi.org/10.7717/peerj.2832 https://doi.org/10.7717/peerj.2832 .

Belcher J H , Swale E M F . 1972 . The morphology and fine structure of the colourless colonial flagellate Anthophysa vegetans (O. F. Müller) Stein . British Phycological Journal , 7 ( 3 ): 335 - 346 , https://doi.org/10.1080/00071617200650351 https://doi.org/10.1080/00071617200650351 .

Bory de Saint-Vincent M . 1822 . Dictionnaire Classique d'Histoire Naturelle. Rey et Gravier, Libraries-Editeurs, Auai des Augusttns . p. 1 - 604 . https://do 10.5962/bhl.title.33901 http://dx.doi.org/10.5962/bhl.title.33901

Dobell C , Leeuwenhoek A V . 1932 . Antony van Leeuwenhoek and his "Little Animals": Being Some Account of the Father of Protozoology and Bacteriology and His Multifarious Discoveries in These Disciplines. Harcourt, Brace and Company, New York . p. 285 - 291 . https://do 10.5962/bhl.title.13354 http://dx.doi.org/10.5962/bhl.title.13354

Drummond A J , Ho S Y W , Phillips M J et al . 2006 . Relaxed phylogenetics and dating with confidence . PLoS Biology , 4 ( 5 ): e 88 , https://doi.org/10.1371/journal.pbio.0040088 https://doi.org/10.1371/journal.pbio.0040088 .

Drummond A J , Rambaut A . 2007 . BEAST: Bayesian evolutionary analysis by sampling trees . BMC Evolutionary Biology , 7 ( 1 ): 214 , https://doi.org/10.1186/1471-2148-7-214 https://doi.org/10.1186/1471-2148-7-214 .

Dujardin F . 1841 . Histoire naturelle des Zoophytes. Infusoires comprenant la physiologie et la classification de ces animaux et la manière de les étudier à l'aide du microscope . Paris, Roret . p. 1 - 684 . https://www.biodiversitylibrary.org/item/40610 https://www.biodiversitylibrary.org/item/40610 . https://do 10.5962/bhl.title.51143 http://dx.doi.org/10.5962/bhl.title.51143

Erasmus A , van Vuuren S J , Levanets A . 2016 . Anthophysa vegetans (O.F. Müller) Stein (Chrysophyceae), a new record from South Africa . Bothalia-African Biodiversity & Conservation , 46 ( 1 ): a 2125 , https://doi.org/10.4102/abc.v46i1.2125 https://doi.org/10.4102/abc.v46i1.2125 .

Hall T A . 1999 . BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95 / 98 /NT. Nucleic Acids Symposium Series, 41 : 95 - 98 .

Hancock F D . 1973 . Algal ecology of a stream polluted through gold mining on the Witwatersrand . Hydrobiologia , 43 ( 1-2 ): 189 - 229 , https://doi.org/10.1007/BF00014267 https://doi.org/10.1007/BF00014267 .

Ho S Y W , Phillips M J . 2009 . Accounting for calibration uncertainty in phylogenetic estimation of evolutionary divergence times . Systematic Biology , 58 ( 3 ): 367 - 380 , https://doi.org/10.1093/sysbio/syp035 https://doi.org/10.1093/sysbio/syp035 .

Jeong M , Kim J I , Nam S W et al . 2021 . Molecular phylogeny and taxonomy of the genus Spumella (Chrysophyceae) based on morphological and molecular evidence. Frontiers in Plant Science , 12 : 758067 , https://doi.org/10.3389/fpls.2021.758067 https://doi.org/10.3389/fpls.2021.758067 .

Jeong M , Kim J I , Shin W . 2025 . Taxonomy of the genus Poterioochromonas (Chrysophyceae) based on morphological and molecular evidence . Journal of Phycology , 61 ( 3 ): 607 - 622 , https://doi.org/10.1111/jpy.70028 https://doi.org/10.1111/jpy.70028 .

Jeong M , Wang Y T , Kim J I et al . 2023 . Multigene phylogeny reveals a cryptic diversity in the genus Dinobryon (Chrysophyceae) with integrative description of five new species. Frontiers in Plant Science , 14 : 1150814 , https://doi.org/10.3389/fpls.2023.1150814 https://doi.org/10.3389/fpls.2023.1150814 .

Joska M A P , Day J A , Boulle J et al . 2005 . Development of a biomonitoring method using protozoans for assessment of water quality in rivers and ground waters and seasonal/ephemeral waters . Water Research Commission Report , 1017 / 1 : 05 .

Kalyaanamoorthy S , Minh B Q , Wong T K F et al . 2017 . ModelFinder: fast model selection for accurate phylogenetic estimates . Nature Methods , 14 ( 6 ): 587 - 589 , https://doi.org/10.1038/nmeth.4285 https://doi.org/10.1038/nmeth.4285 .

Katoh K , Misawa K , Kuma K I et al . 2002 . MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform . Nucleic Acids Research , 30 ( 14 ): 3059 - 3066 , https://doi.org/10.1093/nar/gkf436 https://doi.org/10.1093/nar/gkf436 .

Kim J I , Shin W , Triemer R E . 2013 . Phylogenetic reappraisal of the genus Monomorphina (Euglenophyceae) based on molecular and morphological data . Journal of Phycology , 49 ( 1 ): 82 - 91 , https://doi.org/10.1111/jpy.12018 https://doi.org/10.1111/jpy.12018 .

Lanfear R , Frandsen P B , Wright A M et al . 2017 . PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses . Molecular Biology and Evolution , 34 ( 3 ): 772 - 773 , https://doi.org/10.1093/molbev/msw260 https://doi.org/10.1093/molbev/msw260 .

Lee R E , Kugrens P . 1989 . Biomineralization of the stalks of Anthophysa vegetans (Chrysophyceae) . Journal of Phycology , 25 ( 3 ): 591 - 596 , https://doi.org/10.1111/j.1529-8817.1989.tb00265.x https://doi.org/10.1111/j.1529-8817.1989.tb00265.x .

Müller O F , Fabricius O . 1786. Animalcula Infusoria Fluviatilia et Marina Quæ Detexit, Systematice Descripsit et ad Vivum Delineari Curavit Otho Fridericus Müller; Sistit Opus Hoc Posthumum Quod Cum TABULIS AENEIS L. In Lucem traDit Vidua Ejus Nobilissima Cura Othonis Fabricii . Hauniæ. p. 1 - 367 . https://do 10.5962/bhl.title.129933 http://dx.doi.org/10.5962/bhl.title.129933

Nguyen L T , Schmidt H A , Von Haeseler A et al . 2015 . IQ-TREE: a fast and effective stochastic algorithm for estimating Maximum-Likelihood phylogenies . Molecular Biology and Evolution , 32 ( 1 ): 268 - 274 , https://doi.org/10.1093/molbev/msu300 https://doi.org/10.1093/molbev/msu300 .

Pfeiffer L . 1871 . Nomenclator Botanicus . Nominum ad Finem Anni 1858 Publici Juris Factorum, Classes, Ordines, Tribus, Familias, Divisiones, Genera, Subgenera vel Sectiones Designatium Enumeratio Alphabetica. AdjectisAuctoribus, Temporibus, SystematicisLocis, VariosApud, Notis Literariis Atque Etymologicis et Synonymis. Cassellis, SumptibusT. Fischeri. p . 1 - 808 .

Pringsheim E G . 1946 . On iron flagellates . Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences , 232 ( 588 ): 311 - 342 , https://doi.org/10.1098/rstb.1946.0004 https://doi.org/10.1098/rstb.1946.0004 .

Rambaut A , Drummond A J , Xie D et al . 2018 . Posterior summarization in bayesian phylogenetics using Tracer 1.7 . Systematic Biology , 67 ( 5 ): 901 - 904 , https://doi.org/10.1093/sysbio/syy032 https://doi.org/10.1093/sysbio/syy032 .

Ronquist F , Teslenko M , Van Der Mark P et al . 2012 . MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space . Systematic Biology , 61 ( 3 ): 539 - 542 , https://doi.org/10.1093/sysbio/sys029 https://doi.org/10.1093/sysbio/sys029 .

Roth A W . 1806 . Catalecta botanica: quibus plantae novae et minus cognitae descriuntur atque illustratur. Fasciculus tertius cum tabulis aenaeis XII. in Bibliopolio Io. Fr . Gleditschiano, Lipsiae . p. 1 - 350 .

Rylov W M . 1927 . Über das planktische Vorkommen von Anthophysa steini Senn . Internationale Revue der gesamten Hydrobiologie und Hydrographie , 18 ( 1-2 ): 75 - 84 , https://doi.org/10.1002/iroh.19270180103 https://doi.org/10.1002/iroh.19270180103 .

Saville-Kent W . 1880 . A manual of the Infusoria: Including a Description of all Known Flagellate, Ciliate, and Tentaculiferous Protozoa, British and Foreign, and an Account of the Organization and the Affinities of the Sponges . David Bogue , 3 St. Martin's Place, Trafalgar Square, London. p. 1 - 472 . https://do 10.5962/bhl.title.20943 http://dx.doi.org/10.5962/bhl.title.20943

Senn G G . 1900 . Flagellata . In: Engler A, Prantl K eds. Die natürlichen Pflanzenfamilien . Wilhelm Engelmann, Leipzig. p. 93 - 188 .

Skaloudova M , Skaloud P . 2013 . A new species of Chrysosphaerella (Chrysophyceae: Chromulinales), Chrysosphaerella rotundata sp. nov., from Finland . Phytotaxa , 130 ( 1 ): 34 , https://doi.org/10.11646/phytotaxa.130.1.4 https://doi.org/10.11646/phytotaxa.130.1.4 .

Stein F . 1878 . Der Organismus der Infusionsthiere . Verlag von Wilhhelm Engelmann, Leipzig . p. 1 - 154 .

Stokes A C . 1887 . Notices of new fresh-water infusoria . The American Monthly Microscopical Journal , 8 : 141 - 147 , https://www.biodiversitylibrary.org/page/26707989 https://www.biodiversitylibrary.org/page/26707989 .

Tamura K , Peterson D , Peterson N et al . 2011 . MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods . Molecular Biology and Evolution , 28 ( 10 ): 2731 - 2739 , https://doi.org/10.1093/molbev/msr121 https://doi.org/10.1093/molbev/msr121 .

Wylezich C , Nies G , Mylnikov A P et al . 2010 . An evaluation of the use of the LSU rRNA D1-D5 domain for DNA-based taxonomy of eukaryotic protists . Protist , 161 ( 3 ): 342 - 352 , https://doi.org/10.1016/j.protis.2010.01.003 https://doi.org/10.1016/j.protis.2010.01.003 .

Zhang D , Gao F L , Jakovlić I et al . 2020 . PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies . Molecular Ecology Resources , 20 ( 1 ): 348 - 355 , https://doi.org/10.1111/1755-0998.13096 https://doi.org/10.1111/1755-0998.13096 .

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Phylogenetic study on Scenedesmacae with the description of a new genus Coccoidesmus gen. nov. (Chlorophyceae, Chlorophyta) and chloroplast genome analyses

Shells in the cabinet: four new species of Tellinidae (Mollusca: Bivalvia: Tellinoidea) identified from existing South China Sea collections

Lineaperpetua gen. nov.: a new diatom genus in the Thalassiosirales supported by morphology and molecular data

Neomicrocoleus gen. nov. (Microcoleaceae, Oscillatoriales), a novel cyanobacterial genus from benthic mats in a water channel

Diversity and distribution of oysters from Weizhou Island, China

New molecular sequences illuminate two new Anthophysa species (Chrysophyceae, Ochromonadales) from China: integrating phylogenetic and morphological evidence

New molecular sequences illuminate two new Anthophysa species (Chrysophyceae, Ochromonadales) from China: integrating phylogenetic and morphological evidence

DOI：10.1007/s00343-026-5287-1

摘要

Abstract

关键词

Keywords

references