Community assembly and nitrogen metabolic characteristics of size-fractionated microorganisms in floating bed restoration area

Yi SHI; Rui ZHAO; Lingfeng HUANG; Wenjing ZHANG

doi:10.1007/s00343-025-4336-5

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Community assembly and nitrogen metabolic characteristics of size-fractionated microorganisms in floating bed restoration area

Ecology | Updated：2026-03-26

- Community assembly and nitrogen metabolic characteristics of size-fractionated microorganisms in floating bed restoration area
- Community assembly and nitrogen metabolic characteristics of size-fractionated microorganisms in floating bed restoration area
- 海洋湖沼学报(英文) 2026年44卷第1期页码：184-200
- Affiliations：
  
  1.State Key Laboratory of Marine Environmental Science, Xiamen Key Laboratory of Urban Sea Ecological Conservation and Restoration, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China
  2.Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
- Author bio：
  
  zhangwenjing@xmu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(42141003;42176147);the National Key Research and Development Program of China(2022YFF0802204);the Xiamen Key Laboratory of Urban Sea Ecological Conservation and Restoration (USER)(USER2021-1;USER2021-5)
- DOI：10.1007/s00343-025-4336-5
  中图分类号：
- 收稿：2024-12-10，
  
  录用：2025-03-18，
  
  网络首发：2025-04-07，
  
  纸质出版：2026-01-01
- Accepted：
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Yi SHI, Rui ZHAO, Lingfeng HUANG, 等. Community assembly and nitrogen metabolic characteristics of size-fractionated microorganisms in floating bed restoration area[J]. 海洋湖沼学报(英文), 2026,44(1):184-200.

SHI Yi,ZHAO Rui,HUANG Lingfeng,et al.Community assembly and nitrogen metabolic characteristics of size-fractionated microorganisms in floating bed restoration area[J].Journal of Oceanology and Limnology,2026,44(01):184-200.
Yi SHI, Rui ZHAO, Lingfeng HUANG, 等. Community assembly and nitrogen metabolic characteristics of size-fractionated microorganisms in floating bed restoration area[J]. 海洋湖沼学报(英文), 2026,44(1):184-200. DOI： 10.1007/s00343-025-4336-5.

SHI Yi,ZHAO Rui,HUANG Lingfeng,et al.Community assembly and nitrogen metabolic characteristics of size-fractionated microorganisms in floating bed restoration area[J].Journal of Oceanology and Limnology,2026,44(01):184-200. DOI： 10.1007/s00343-025-4336-5.

摘要

Abstract

Ecological floating bed is an important biological remediation method for water pollution control. During the removal of excess nutrients and pollutants

changes in environmental factors affect the characteristics of microorganisms in aquatic ecosystems. To understand the influences of ecological floating beds on size-fractionated microorganisms

we investigated the community assembly and nitrogen metabolic characteristics of three size-fractionated microorganism groups in the ecological floating bed area

using 18S rDNA

16S rDNA metabarcoding

and metagenomic sequencing techniques. Firstly

we discovered substantial differences between size-fractionated groups in the diversity and compositions of both microeukaryotic and bacterial communities

as well as the influences of floating beds on specific groups. The floating beds appeared to provide more habitats for heterotrophs and symbiotes while potentially inhibiting the growth of certain phytoplankton (cyanobacteria). Secondly

we observed that microeukaryotic and bacterial communities were predominantly influenced by stochastic and deterministic processes

respectively

and they both exhibited distinct patterns across different size-fractionated groups. Notably

microeukaryotic community assembly demonstrated a greater sensitivity to ecological floating beds

as indicated by an increase in dispersal limitation processes. Finally

the nitrogen metabolism functional genes revealed that microbes associated with large-sized particles played a crucial role in dissimilatory nitrate reduction to ammonium (DNRA) and denitrification processes within the floating bed area

thereby facilitating the removal of excess nitrogen nutrients from the water. In contrast

free-living microorganisms from small-sized groups were linked mainly to the genes involved in nitrogen assimilation and assimilatory nitrate reduction to ammonium (ANRA) processes. These findings help understand the impact of ecological floating beds on the diversity and functional characteristics of microorganism communities in different size-fractionated groups.

关键词

Keywords

references

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