Transcriptomic dynamics in the hepatopancreas of <italic style="font-style: italic">Macrobrachium</italic> <italic style="font-style: italic">rosenbergii</italic> during thermal elimination of infection with Decapod Iridescent Virus 1

Xingyu ZHENG; Miaoying CAI; Qi SHEN; Liping LI; Zhenglong XIA; Quanxin GAO; Qiongying TANG; Guoliang YANG; Jiongying YU; Shaokui YI

doi:10.1007/s00343-025-4256-4

Your Location：

Home >

Browse articles >

Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1

Aquaculture and Fisheries | Updated：2026-03-26

- Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1
- Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1
- 海洋湖沼学报(英文) 2026年44卷第1期页码：445-459
- Affiliations：
  
  1.School of Life Sciences, Huzhou University, Huzhou 313000, China
  2.Jiangsu Shufeng Prawn Breeding Co., Ltd., Gaoyou 225654, China
- Author bio：
  
  yujiongying@outlook.com
  yishaokui@foxmail.com
- Funds：
  
  the earmarked fund for the China Agriculture Research System of MOF and MARA(CARS-48);the National Natural Science Foundation of China(32202964)
- DOI：10.1007/s00343-025-4256-4
  中图分类号：
- 收稿：2024-09-24，
  
  录用：2025-01-09，
  
  网络首发：2025-05-14，
  
  纸质出版：2026-01-01
- Accepted：
Scan QR Code
Xingyu ZHENG, Miaoying CAI, Qi SHEN, 等. Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1[J]. 海洋湖沼学报(英文), 2026,44(1):445-459.

ZHENG Xingyu,CAI Miaoying,SHEN Qi,et al.Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1[J].Journal of Oceanology and Limnology,2026,44(01):445-459.
Xingyu ZHENG, Miaoying CAI, Qi SHEN, 等. Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1[J]. 海洋湖沼学报(英文), 2026,44(1):445-459. DOI： 10.1007/s00343-025-4256-4.

ZHENG Xingyu,CAI Miaoying,SHEN Qi,et al.Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1[J].Journal of Oceanology and Limnology,2026,44(01):445-459. DOI： 10.1007/s00343-025-4256-4.

摘要

Abstract

Decapod Iridescent Virus 1 (DIV1) is a recently discovered virus recognized for its high infectivity in

Macrobrachium

rosenbergii

. A thermal treatment was performed on DIV1-infected

rosenbergii

and the therapeutic efficacy was evaluated. In the DIV1 challenge experiment

the mortality rate in the challenged group was found to be 2.6 times greater than that in the control group

with the vir

al load in deceased individuals exceeding 5.41×10

copies/μg-DNA. The thermal treatment (TT) was administered at 36 °C for a duration of 16 d

followed by a temperature restoration (TR) period at 26 °C for 3 d. On the first day at 36 °C

an average viral concentration of 5.34×10 copies/μg-DNA

was detected in the survived individuals. RNA-seq analysis showed a significant upregulation of genes related to the lysosome pathway

including sialin-like isoform x2 (

slc17a5

)

beta-galactosidase-1-like protein 2 (

glb1

)

putative glucosylceramidase 3 (

gba

)

sphingomyelin phosphodiesterase-like isoform x2 (

smpd1

)

beta-hexosaminidase subunit alpha-like (

hexa_b

) and legumain-like protein (

lgmn

)

following a transient suppression period induced by thermal stress. Upon reaching 36 °C

the activation of heat shock protein 70 (

hsp70

) and heat shock protein 90 (

hsp90a

) was observed. Concomitantly

genes that implicated in energy production critical for DIV1 replication

such as hexokinase (

) and microsomal glutathione s-transferase 3-like isoform x2 (

gst

)

were inhibited. These results collectively suggest that TT/TR treatments eliminated DIV1 in

rosenbergii

by activating the organism’s innate immune response and suppressing virus replication. This study provides a theoretical basis for utilizing thermal therapy in the management of viral infections in

rosenbergii

breeding programs

thereby facilitating the development of new strains resistant to DIV1.

关键词

Keywords

references

Anders S , Huber W . 2010 . Differential expression analysis for sequence count data . Genome Biology , 11 ( 10 ): R 106 , https://doi.org/10.1186/gb-2010-11-10-r106 https://doi.org/10.1186/gb-2010-11-10-r106 .

Bir J , Sarker H , Mita F S et al . 2024 . The impact of salinity and temperature stress on survival, behaviour, immune response, and proximate composition of giant freshwater prawn Macrobrachium rosenbergii . Aquaculture International , 32 ( 5 ): 6333 - 6352 , https://doi.org/10.1007/s10499-024-01468-6 https://doi.org/10.1007/s10499-024-01468-6 .

Bo Y Y , Liang L D , Hua Y J et al . 2021 . High-purity DNA extraction from animal tissue using picking in the trizol-based method . Biotechniques , 70 ( 3 ): 186 - 190 , https://doi.org/10.2144/btn-2020-0142 https://doi.org/10.2144/btn-2020-0142 .

Chen X , Qiu L , Wang H L et al . 2019 . Susceptibility of Exopalaemon carinicauda to the infection with shrimp hemocyte iridescent virus (SHIV 20141215), a strain of decapod iridescent virus 1 (DIV1) . Viruses , 11 ( 4 ): 387 , https://doi.org/10.3390/v11040387 https://doi.org/10.3390/v11040387 .

Davidson N M , Oshlack A . 2014 . Corset: enabling differential gene expression analysis for de novo assembled transcriptomes . Genome Biology , 15 ( 7 ): 410 , https://doi.org/10.1186/s13059-014-0410-6 https://doi.org/10.1186/s13059-014-0410-6 .

Futschik M E , Carlisle B . 2005 . Noise-robust soft clustering of gene expression time-course data . Journal of Bioinformatics and Computational Biology , 3 ( 4 ): 965 - 988 , https://doi.org/10.1142/S0219720005001375 https://doi.org/10.1142/S0219720005001375 .

Grabherr M G , Haas B J , Yassour M et al . 2011 . Full-length transcriptome assembly from RNA-seq data without a reference genome . Nature Biotechnology , 29 ( 7 ): 644 - 652 , https://doi.org/10.1038/nbt.1883 https://doi.org/10.1038/nbt.1883 .

Guo X M , Qiu L , Gao W et al . 2022 . Radical thermal therapy against infection with decapod iridescent virus 1 (DIV1). Aquaculture , 561 : 738636 , https://doi.org/10.1016/j.aquaculture.2022.738636 https://doi.org/10.1016/j.aquaculture.2022.738636 .

He Z H , Chen X Y , Zhao J C et al . 2021a . Establishment of infection mode and Penaeus monodon hemocytes transcriptomics analysis under decapod iridescent virus 1 (DIV1) challenge. Aquaculture , 542 : 736816 , https://doi.org/10.1016/j.aquaculture.2021.736816 https://doi.org/10.1016/j.aquaculture.2021.736816 .

He Z H , Zhao J C , Chen X Y et al . 2021b . The molecular mechanism of hemocyte immune response in Marsupenaeus japonicus infected with decapod iridescent virus 1. Frontiers in Microbiology , 12 : 710845 , https://doi.org/10.3389/fmicb.2021.710845 https://doi.org/10.3389/fmicb.2021.710845 .

Hong T J , Hahn J S . 2016 . Application of SGT1-Hsp90 chaperone complex for soluble expression of NOD1 LRR domain in E . coli . Biochemical and Biophysical Research Communications , 478 ( 4 ): 1647 - 1652 , https://doi.org/10.1016/j.bbrc.2016.08.174 https://doi.org/10.1016/j.bbrc.2016.08.174 .

Jiang F J , Yue X , Wang H X et al . 2017 . Transcriptome profiles of the clam Meretrix petechialis hepatopancreas in response to Vibrio infection . Fish & Shellfish Immunology , 62 : 175 - 183 , https://doi.org/10.1016/j.fsi.2017.01.024 https://doi.org/10.1016/j.fsi.2017.01.024 .

Ju-Ngam T , McMillan N , Yoshimizu M et al . 2021 . Functional and stress response analysis of heat shock proteins 40 and 90 of giant river prawn ( Macrobrachium rosenbergii ) under temperature and pathogenic bacterial exposure stimuli . Biomolecules , 11 ( 7 ): 1034 , https://doi.org/10.3390/biom11071034 https://doi.org/10.3390/biom11071034 .

Kulkarni A , Krishnan S , Anand D et al . 2021 . Immune responses and immunoprotection in crustaceans with special reference to shrimp . Reviews in Aquaculture , 13 ( 1 ): 431 - 459 , https://doi.org/10.1111/raq.12482 https://doi.org/10.1111/raq.12482 .

Langfelder P , Horvath S . 2008 . WGCNA: an R package for weighted correlation network analysis . BMC Bioinformatics , 9 ( 1 ): 559 , https://doi.org/10.1186/1471-2105-9-559 https://doi.org/10.1186/1471-2105-9-559 .

Li B , Dewey C N . 2011 . RSEM: accurate transcript quantification from RNA-seq data with or without a reference genome . BMC Bioinformatics , 12 ( 1 ): 323 , https://doi.org/10.1186/1471-2105-12-323 https://doi.org/10.1186/1471-2105-12-323 .

Li X H , Cui Z X , Liu Y et al . 2013 . Transcriptome analysis and discovery of genes involved in immune pathways from hepatopancreas of microbial challenged mitten crab Eriocheir sinensis . PLoS One , 8 ( 7 ): e 68233 , https://doi.org/10.1371/journal.pone.0068233 https://doi.org/10.1371/journal.pone.0068233 .

Li X P , Luan S , Luo K et al . 2022 . Comparative transcriptomic analysis of Chinese shrimp Fenneropenaeus chinensis infected with white spot syndrome virus. Aquaculture Reports , 22 : 100986 , https://doi.org/10.1016/j.aqrep.2021.100986 https://doi.org/10.1016/j.aqrep.2021.100986 .

Liao X Z , He J G , Li C Z . 2022a . Decapod iridescent virus 1: an emerging viral pathogen in aquaculture . Reviews in Aquaculture , 14 ( 4 ): 1779 - 1789 , https://doi.org/10.1111/raq.12672 https://doi.org/10.1111/raq.12672 .

Liao X Z , Wang C G , Wang B et al . 2020b . Research into the hemocyte immune response of Fenneropenaeus merguiensis under decapod iridescent virus 1 (DIV1) challenge using transcriptome analysis . Fish & Shellfish Immunology , 104 : 8 - 17 , https://doi.org/10.1016/j.fsi.2020.05.053 https://doi.org/10.1016/j.fsi.2020.05.053 .

Love M I , Huber W , Anders S . 2014 . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 . Genome Biology , 15 ( 12 ): 550 , https://doi.org/10.1186/s13059-014-0550-8 https://doi.org/10.1186/s13059-014-0550-8 .

Lu Y P , Tu C , Wu C et al . 2023 . Emergence of Decapod iridescent virus 1 in cultured shrimp from Taiwan in 2020 . Veterinary Medicine and Science , 9 ( 5 ): 2336 - 2341 , https://doi.org/10.1002/vms3.1216 https://doi.org/10.1002/vms3.1216 .

Mao X Z , Cai T , Olyarchuk J G et al . 2005 . Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary . Bioinformatics , 21 ( 19 ): 3787 - 3793 , https://doi.org/10.1093/bioinformatics/bti430 https://doi.org/10.1093/bioinformatics/bti430 .

Montgomery-Brock D , Tacon A G J , Poulos B et al . 2007 . Reduced replication of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in Litopenaeus vannamei held in warm water . Aquaculture , 265 ( 1-4 ): 41 - 48 , https://doi.org/10.1016/j.aquaculture.2007.01.025 https://doi.org/10.1016/j.aquaculture.2007.01.025 .

Network of Aquaculture Centres in Asia-Pacific . 2020 . Infection with decapod iridescent virus 1 (DIV 1 ): disease card. Network of Aquaculture Centres in Asia-Pacific Release, 7 May 2020 , https://enaca.org/?id=1104. Accessed on 2025-01-19 https://enaca.org/?id=1104.Accessedon2025-01-19 .

Ng Y S , Lee D Y , Liu C H et al . 2022 . White spot syndrome virus triggers a glycolytic pathway in shrimp immune cells (hemocytes) to benefit its replication. Frontiers in Immunology , 13 : 901111 , https://doi.org/10.3389/fimmu.2022.901111 https://doi.org/10.3389/fimmu.2022.901111 .

Perazzolo L M , Li C Z , Somboonwiwat K . 2021 . Editorial: aquatic invertebrate immunity against infectious diseases. Frontiers in Immunology , 12 : 762082 , https://doi.org/10.3389/fimmu.2021.762082 https://doi.org/10.3389/fimmu.2021.762082 .

Qian Q Q , Zhou Y F , Chen Z et al . 2023 . Pathogenesis and complete genome sequence of Decapod iridescent virus 1 (DIV1) associated with mass mortality in Macrobrachium rosenbergii . Aquaculture , 566 : 739220 , https://doi.org/10.1016/j.aquaculture.2022.739220 https://doi.org/10.1016/j.aquaculture.2022.739220 .

Qiu L , Chen M M , Wan X Y et al . 2017 . Characterization of a new member of Iridoviridae , shrimp hemocyte iridescent virus (SHIV), found in white leg shrimp ( Litopenaeus vannamei ) . Scientific Reports , 7 ( 1 ): 11834 , https://doi.org/10.1038/s41598-017-10738-8 https://doi.org/10.1038/s41598-017-10738-8 .

Qiu L , Chen M M , Wan X Y et al . 2018 . Detection and quantification of shrimp hemocyte iridescent virus by TaqMan probe based real-time PCR . Journal of Invertebrate Pathology , 154 : 95 - 101 , https://doi.org/10.1016/j.jip.2018.04.005 https://doi.org/10.1016/j.jip.2018.04.005 .

Qiu L , Chen X , Zhao R H et al . 2019 . Description of a natural infection with Decapod iridescent virus 1 in farmed giant freshwater prawn, Macrobrachium rosenbergii . Viruses , 11 ( 4 ): 354 , https://doi.org/10.3390/v11040354 https://doi.org/10.3390/v11040354 .

Rahman M M , Escobedo-Bonilla C M , Corteel M et al . 2006 . Effect of high water temperature (33°C) on the clinical and virological outcome of experimental infections with white spot syndrome virus (WSSV) in specific pathogen-free (SPF) Litopenaeus vannamei . Aquaculture , 261 ( 3 ): 842 - 849 , https://doi.org/10.1016/j.aquaculture.2006.09.007 https://doi.org/10.1016/j.aquaculture.2006.09.007 .

Rasid R , Tajuddin A L , Zarim M A U A A et al . 2023 . Tolerance limit of physicochemical water parameters in giant freshwater prawn ( Macrobrachium rosenbergii ) in a captive condition . Turkish Journal of Zoology , 47 ( 5 ): 291 - 298 , https://doi.org/10.55730/1300-0179.3142 https://doi.org/10.55730/1300-0179.3142 .

Ren Q , Zhang X W , Sun Y D et al . 2010 . Two cysteine proteinases respond to bacterial and WSSV challenge in Chinese white shrimp Fenneropenaeus chinensis . Fish & Shellfish Immunology , 29 ( 4 ): 551 - 556 , https://doi.org/10.1016/j.fsi.2010.03.002 https://doi.org/10.1016/j.fsi.2010.03.002 .

Rőszer T . 2014 . The invertebrate midintestinal gland (“hepatopancreas”) is an evolutionary forerunner in the integration of immunity and metabolism . Cell and Tissue Research , 358 ( 3 ): 685 - 695 , https://doi.org/10.1007/s00441-014-1985-7 https://doi.org/10.1007/s00441-014-1985-7 .

Shukla M , Narayan M . 2025 . Proteostasis and its role in disease development . Cell Biochemistry and Biophysics , 83 ( 2 ): 1725 - 1741 , https://doi.org/10.1007/s12013-024-01581-6 https://doi.org/10.1007/s12013-024-01581-6 .

Sodowich B I , Fadl I , Burns C . 2007 . Method validation of in vitro RNA transcript analysis on the Agilent 2100 Bioanalyzer . Electrophoresis , 28 ( 14 ): 2368 - 2378 , https://doi.org/10.1002/elps.200600673 https://doi.org/10.1002/elps.200600673 .

Soto M A , Lotz J M . 2001 . Epidemiological parameters of white spot syndrome virus infections in Litopenaeus vannamei and L . setiferus . Journal of Invertebrate Pathology , 78 ( 1 ): 9 - 15 , https://doi.org/10.1006/jipa.2001.5035 https://doi.org/10.1006/jipa.2001.5035 .

Srisala J , Sanguanrut P , Thaiue D et al . 2021 . Infectious myonecrosis virus (IMNV) and Decapod iridescent virus 1 (DIV1) detected in captured, wild Penaeus monodon . Aquaculture , 545 : 737262 , https://doi.org/10.1016/j.aquaculture.2021.737262 https://doi.org/10.1016/j.aquaculture.2021.737262 .

Storey J D . 2003 . The positive false discovery rate: a Bayesian interpretation and the q -value . The Annals of Statistics , 31 ( 6 ): 2013 - 2035 , https://doi.org/10.1214/aos/1074290335 https://doi.org/10.1214/aos/1074290335 .

Sun Y M , Li F H , Sun Z et al . 2014a . Transcriptome analysis of the initial stage of acute WSSV infection caused by temperature change . PLoS One , 9 ( 3 ): e 90732 , https://doi.org/10.1371/journal.pone.0090732 https://doi.org/10.1371/journal.pone.0090732 .

Sun Z B , Yang C Y , Wang L L et al . 2014b . The protein expression profile in hepatopancreas of scallop Chlamys farreri under heat stress and Vibrio anguillarum challenge . Fish & Shellfish Immunology , 36 ( 1 ): 252 - 260 , https://doi.org/10.1016/j.fsi.2013.11.008 https://doi.org/10.1016/j.fsi.2013.11.008 .

Wang Y , Dai L X , Liang Z L et al . 2024 . Elimination of Decapod iridovirus 1 (DIV1) infection at high water temperature: a new environmental control strategy . Advanced Biotechnology , 2 ( 2 ): 12 , https://doi.org/10.1007/s44307-024-00012-0 https://doi.org/10.1007/s44307-024-00012-0 .

Withyachumnarnkul B , Boonsaeng V , Chomsoong R et al . 2003 . Seasonal variation in white spot syndrome virus-positive samples in broodstock and post-larvae of Penaeus monodon in Thailand . Diseases of Aquatic Organisms , 53 ( 2 ): 167 - 171 , https://doi.org/10.3354/dao053167 https://doi.org/10.3354/dao053167 .

Wu X G , Xiong H T , Wang Y Z et al . 2012 . Evidence for cell apoptosis suppressing white spot syndrome virus replication in Procambarus clarkii at high temperature . Diseases of Aquatic Organisms , 102 ( 1 ): 13 - 21 , https://doi.org/10.3354/dao02532 https://doi.org/10.3354/dao02532 .

Xu L M , Wang T T , Li F et al . 2016 . Isolation and preliminary characterization of a new pathogenic iridovirus from redclaw crayfish Cherax quadricarinatus . Diseases of Aquatic Organisms , 120 ( 1 ): 17 - 26 , https://doi.org/10.3354/dao03007 https://doi.org/10.3354/dao03007 .

You X X , Su Y Q , Mao Y et al . 2010 . Effect of high water temperature on mortality, immune response and viral replication of WSSV-infected Marsupenaeus japonicus juveniles and adults . Aquaculture , 305 ( 1-4 ): 133 - 137 , https://doi.org/10.1016/j.aquaculture.2010.04.024 https://doi.org/10.1016/j.aquaculture.2010.04.024 .

Young M D , Wakefield M J , Smyth G K et al . 2010 . Gene ontology analysis for RNA-seq: accounting for selection bias . Genome Biology , 11 ( 2 ): R 14 , https://doi.org/10.1186/gb-2010-11-2-r14 https://doi.org/10.1186/gb-2010-11-2-r14 .

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation

Genetic parameter evaluation for growth and cold-resistance traits of the giant freshwater prawn Macrobrachium rosenbergii

Alternative splicing genes/events play important role in adaptation of mud crab Scylla paramamosain to low salinity

Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1

Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1

DOI：10.1007/s00343-025-4256-4

摘要

Abstract

关键词

Keywords

references