Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish <italic style="font-style: italic">Siniperca</italic> <italic style="font-style: italic">chuatsi</italic>

Xiaohua JI; Xiaomi ZHANG; Yuchong SUN; Zizhao FENG; Fei YU; Dan XU; Liqun LU

doi:10.1007/s00343-025-4239-5

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Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish Siniperca chuatsi

Aquaculture and Fisheries | Updated：2025-12-16

- Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish Siniperca chuatsi
- Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish Siniperca chuatsi
- 海洋湖沼学报(英文) 2025年43卷第6期页码：1974-1984
- Affiliations：
  
  1.National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China
  2.Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210024, China
  3.Key Laboratory of Agriculture Ministry for Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai 201306, China
- Author bio：
  
  lqlv@shou.edu.cn
- Funds：
  
  the Earmarked Fund for China Agricultural Research System(CARS-45-16);the Key Research and Development Plan of Jiangsu Province(BE2021369)
- DOI：10.1007/s00343-025-4239-5
  中图分类号：
- 收稿：2024-09-13，
  
  纸质出版：2025-11-01
- Accepted：
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Xiaohua JI, Xiaomi ZHANG, Yuchong SUN, 等. Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish Siniperca chuatsi[J]. 海洋湖沼学报(英文), 2025,43(6):1974-1984.

JI Xiaohua,ZHANG Xiaomi,SUN Yuchong,et al.Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish ,Siniperca ,chuatsi[J].Journal of Oceanology and Limnology,2025,43(06):1974-1984.
Xiaohua JI, Xiaomi ZHANG, Yuchong SUN, 等. Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish Siniperca chuatsi[J]. 海洋湖沼学报(英文), 2025,43(6):1974-1984. DOI： 10.1007/s00343-025-4239-5.

JI Xiaohua,ZHANG Xiaomi,SUN Yuchong,et al.Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish ,Siniperca ,chuatsi[J].Journal of Oceanology and Limnology,2025,43(06):1974-1984. DOI： 10.1007/s00343-025-4239-5.

摘要

Abstract

Mass mortality of mandarin fish (

Siniperca

chuatsi

) due to infectious spleen and kidney necrosis virus (ISKNV) infection occurs frequently. Since there are no effective drug treatments available

prevention relies heavily on detection. Effective and rapid on-site detection methods are needed for early diagnosis of ISKNV. In this study

a rapid and simple colloidal gold test strip method

specific for the antibody against major capsid protein (MCP)

was developed and systematically evaluated for the detection of ISKNV. The limit of detection of the test strip is a 1꞉100 dilution of a positive standard serum and the antibody level in the fish could be estimated from the depth of color of the test line. The strips were tested against serum samples of cyprinid herpesvirus-2 (CyHV-2)

grass carp reovirus (GCRV)

largemouth bass ranavirus (LMBV)

large yellow croaker iridovirus (LYCIV)

and spring viremia of carp virus (SVCV)

yielding no cross-reactivity. In addition

10 mandarin fish artificially infected with ISKNV were tested using the current industry standard PCR method (SC/T 7211-2011) on their splenorenal tissues. The results from the test strips showed a high degree of c

oncordance with PCR testing

achieving a Kappa value of 0.737. All the results indicated that the colloidal gold test strips prepared in this study could be used as a simple

rapid

and highly sensitive and specific method for ISKNV diagnosis.

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Keywords

references

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Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish Siniperca chuatsi

Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish Siniperca chuatsi

DOI：10.1007/s00343-025-4239-5

摘要

Abstract

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