S-nitrosylation coordination enhances high salt tolerance in <italic style="font-style: italic">Ulva prolifera</italic>

Dongren ZHANG; Xiuwen YANG; Jingwei DONG; Hongyan HE; Aurang ZEB; Ruyi SONG; Bing HAN; Songdong SHEN

doi:10.1007/s00343-025-4163-8

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S-nitrosylation coordination enhances high salt tolerance in Ulva prolifera

Biology | Updated：2025-10-16

- S-nitrosylation coordination enhances high salt tolerance in Ulva prolifera
- S-nitrosylation coordination enhances high salt tolerance in Ulva prolifera
- 海洋湖沼学报(英文) 2025年43卷第5期页码：1553-1566
- Affiliations：
  
  School of Basic Medical Sciences, Soochow University, Suzhou 215101, China
- Author bio：
  
  shensongdong@suda.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(42276100);the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- DOI：10.1007/s00343-025-4163-8
  中图分类号：
- 收稿：2024-01-05，
  
  录用：2024-05-13，
  
  网络首发：2024-11-01，
  
  纸质出版：2025-09-01
- Accepted：
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S-nitrosylation coordination enhances high salt tolerance in Ulva prolifera[J]. 海洋湖沼学报(英文), 2025,43(5):1553-1566.

ZHANG Dongren,YANG Xiuwen,DONG Jingwei,et al.S-nitrosylation coordination enhances high salt tolerance in Ulva prolifera[J].Journal of Oceanology and Limnology,2025,43(05):1553-1566.
S-nitrosylation coordination enhances high salt tolerance in Ulva prolifera[J]. 海洋湖沼学报(英文), 2025,43(5):1553-1566. DOI： 10.1007/s00343-025-4163-8.

ZHANG Dongren,YANG Xiuwen,DONG Jingwei,et al.S-nitrosylation coordination enhances high salt tolerance in Ulva prolifera[J].Journal of Oceanology and Limnology,2025,43(05):1553-1566. DOI： 10.1007/s00343-025-4163-8.

摘要

Abstract

Ulva prolifera

the primary causative species of green tide

has garnered significant attention due to its robust growth and reproductive capacity under high salt stress. However

there has been relatively little research on the regulation of high salt stress in this species. In this study

we observed that high salt stress suppressed the growth of

prolifera

and leading to the nitric oxide (NO) accumulation

along with increased gene expression levels and enzyme activity of S-nitrosoglutathione reductase (GSNOR). Treatment with GSNOR inhibitor resulted in elevated NO levels under high salt stress

accompanied by reduced activity of antioxidant enzymes and decreased glutathione (GSH) accumulation

making

prolifera

more sensitive to high salt stress. Conversely

NO scavenger treatment not only reduced NO levels

but also weakened the high salt stress tolerance of

prolifera

. Furthermore

using tandem mass tags (TMT) switch analysis and mass spectrometry

we observed a significant increase in S-nitrosylated protein levels in

prolifera

under high salt stress

with further augmentation upon GSNOR inhibitor treatment. We also found high salt stress induced S-nitrosylation (SNO) of glutathione reductase (GR)

which is negati

vely regulated by GSNOR

resulting in increased GR activity. Our results show that under short-term high salt stress

the elevated expression level of GSNOR avoided excessive accumulation of NO

and a certain amount of NO enhanced the activity of antioxidant enzymes through SNO modification

which improve the high salt stress tolerance of

prolifera

whereas under long-term high salt stress

excessive NO was toxic to

prolifera

关键词

Keywords

references

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S-nitrosylation coordination enhances high salt tolerance in Ulva prolifera

S-nitrosylation coordination enhances high salt tolerance in Ulva prolifera

DOI：10.1007/s00343-025-4163-8

摘要

Abstract

关键词

Keywords

references