Silicon limitation affects diatom’s resistance to copepod grazing

Huo XU; Fengyuan CHEN; Minqi LUO; Xiaodong ZHANG; Ke PAN; Hongbin LIU

doi:10.1007/s00343-024-4142-5

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Silicon limitation affects diatom’s resistance to copepod grazing

Ecology | Updated：2025-08-07

- Silicon limitation affects diatom’s resistance to copepod grazing
- Silicon limitation affects diatom’s resistance to copepod grazing
- 海洋湖沼学报(英文) 2025年43卷第4期页码：1201-1212
- Affiliations：
  
  1.SZU‐HKUST Joint PhD Program in Marine Environmental Science, Shenzhen University, Shenzhen 518000, China
  2.Department of Ocean Science, The Hong Kong University of Science and Technology, Hong Kong SAR 999077, China
  3.Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518000, China
  4.Hong Kong Branch of Southern Marine Science & Engineering Guangdong Laboratory, The Hong Kong University of Science and Technology, Hong Kong SAR 999077, China
- Author bio：
  
  panke@szu.edu.cn
  liuhb@ust.hk
- Funds：
  
  the National Natural Science Foundation of China(U23A2048;42376152;42076148);the Special Program of Key Sectors in Guangdong Universities(2022ZDZX4040;2023KCXTD028)
- DOI：10.1007/s00343-024-4142-5
  中图分类号：
- 收稿：2024-06-05，
  
  纸质出版：2025-07-01
- Accepted：
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Silicon limitation affects diatom’s resistance to copepod grazing[J]. 海洋湖沼学报(英文), 2025,43(4):1201-1212.

XU Huo,CHEN Fengyuan,LUO Minqi,et al.Silicon limitation affects diatom’s resistance to copepod grazing[J].Journal of Oceanology and Limnology,2025,43(04):1201-1212.
Silicon limitation affects diatom’s resistance to copepod grazing[J]. 海洋湖沼学报(英文), 2025,43(4):1201-1212. DOI： 10.1007/s00343-024-4142-5.

XU Huo,CHEN Fengyuan,LUO Minqi,et al.Silicon limitation affects diatom’s resistance to copepod grazing[J].Journal of Oceanology and Limnology,2025,43(04):1201-1212. DOI： 10.1007/s00343-024-4142-5.

摘要

Abstract

Silicon limitation negatively affects the growth and metabolism of diatoms. However

its influence on the topography and mechanical properties of diatom frustules

and consequently on predation

remains unclear. We investigated how silicon limitation affects the mechanical strength of diatom frustules. Under silicon limitation

the growth rates of diatom

Cylindrotheca

closterium

Amphora

coffeaeformis

Thalassiosira

weissflogii

and

Cyclotella

sp. decreased by 19%

56%

42%

and 73%

respectively. Similarly

the biogenic silica content of silicon-limited

closterium

weissflogii

and

Cyclotella

sp. decreased by 17%

11%

and 9%

respectively

whereas

coffeaeformis

showed a 63% increase. Atomic force microscopy and X-ray photoelectron spectroscopy rev

ealed that silicon shortage reduced frustule hardness by approximately 60% and decreased condensed silica components on their surface by about 80%

except in

coffeaeformis

. Additionally

copepods consumed 20% to 600% more diatoms grown under silicon deficiency compared to those grown under Si-rich conditions

with the exception of

coffeaeformis

. These findings suggest that silicon limitation diminishes diatom populations and accelerates carbon export from diatoms to the deep sea.

关键词

Keywords

references

Allen J T , Brown L , Sanders R et al . 2005 . Diatom carbon export enhanced by silicate upwelling in the northeast Atlantic . Nature , 437 ( 7059 ): 728 - 732 , https://doi.org/10.1038/nature03948 https://doi.org/10.1038/nature03948 .

Amo Y D , Brzezinski M A . 1999 . The chemical form of dissolved Si taken up by marine diatoms . Journal of Phycology , 35 ( 6 ): 1162 - 1170 , https://doi.org/10.1046/j.1529-8817.1999.3561162.x https://doi.org/10.1046/j.1529-8817.1999.3561162.x .

Brzezinski M A , Olson R J , Chisholm S W . 1990 . Silicon availability and cell-cycle progression in marine diatoms . Marine Ecology Progress Series , 67 ( 1 ): 83 - 96 , https://doi.org/10.3354/meps067083 https://doi.org/10.3354/meps067083 .

Chen F Y , Ma J , Zhong Z H et al . 2023 . Silicon limitation impairs the tolerance of marine diatoms to pristine microplastics . Environmental Science & Technology , 57 ( 8 ): 3291 - 3300 , https://doi.org/10.1021/acs.est.2c09305 https://doi.org/10.1021/acs.est.2c09305 .

Claquin P , Martin-Jézéquel V , Kromkamp J C et al . 2002 . Uncoupling of silicon compared with carbon and nitrogen metabolisms and the role of the cell cycle in continuous cultures of Thalassiosira pseudonana (bacillariophyceae) under light, nitrogen, and phosphorus control . Journal of Phycology , 38 ( 5 ): 922 - 930 , https://doi.org/10.1046/j.1529-8817.2002.t01-1-01220.x https://doi.org/10.1046/j.1529-8817.2002.t01-1-01220.x .

Davidson K , Gowen R J , Tett P et al . 2012 . Harmful algal blooms: how strong is the evidence that nutrient ratios and forms influence their occurrence? Estuarine, Coastal and Shelf Science , 115 : 399 - 413 , https://doi.org/10.1016/j.ecss.2012.09.019 https://doi.org/10.1016/j.ecss.2012.09.019 .

Du C , Liang J R , Chen D D et al . 2014 . iTRAQ-based proteomic analysis of the metabo lism mechanism associated with silicon response in the marine diatom Thalassiosira pseudonana . Journal of Proteome Research , 13 ( 2 ): 720 - 734 , https://doi.org/10.1021/pr400803w https://doi.org/10.1021/pr400803w .

Dugdale R C , Wilkerson F P , Minas H J . 1995 . The role of a silicate pump in driving new production . Deep Sea Research Part I : Oceanographic Research Papers , 42 ( 5 ): 697 - 719 , https://doi.org/10.1016/0967-0637(95)00015-X https://doi.org/10.1016/0967-0637(95)00015-X .

Field C B , Behrenfeld M J , Randerson J T et al . 1998 . Primary production of the biosphere: integrating terrestrial and oceanic components . Science , 281 ( 5374 ): 237 - 240 , https://doi.org/10.1126/science.281.5374.237 https://doi.org/10.1126/science.281.5374.237 .

Finkel Z V , Kotrc B . 2010 . Silica use through time: macroevolutionary change in the morphology of the diatom fustule . Geomicrobiology Journal , 27 ( 6-7 ): 596 - 608 , https://doi.org/10.1080/01490451003702941 https://doi.org/10.1080/01490451003702941 .

Frost B W . 1972 . Effects of size and concentration of food particles on the feeding behavior of the marine planktonic copepod Calanus pacificus . Limnology and Oceanography , 17 ( 6 ): 805 - 815 , https://doi.org/10.4319/lo.1972.17.6.0805 https://doi.org/10.4319/lo.1972.17.6.0805 .

Goldenberg S U , Taucher J , Fernández-Méndez M et al . 2022 . Nutrient composition (Si:N) as driver of plankton communities during artificial upwelling. Frontiers in Marine Science , 9 : 1015188 , https://doi.org/10.3389/fmars.2022.1015188 https://doi.org/10.3389/fmars.2022.1015188 .

Gong G C , Chang J , Chiang K P et al . 2006 . Reduction of primary production and changing of nutrient ratio in the East China Sea: effect of the Three Gorges Dam? Geophysical Research Letters , 33 ( 7 ): L 07610 , https://doi.org/10.1029/2006GL025800 https://doi.org/10.1029/2006GL025800 .

Grasshoff K , Kremling K , Ehrhardt M . 2009 . Methods of Seawater Analysis . John Wiley & Sons .

Kranzler C F , Krause J W , Brzezinski M A et al . 2019 . Silicon limitation facilitates virus infection and mortality of marine diatoms . Nature Microbiology , 4 ( 11 ): 1790 - 1797 , https://doi.org/10.1038/s41564-019-0502-x https://doi.org/10.1038/s41564-019-0502-x .

Krause J W , Schulz I K , Rowe K A et al . 2019 . Silicic acid limitation drives bloom termination and potential carbon sequestration in an Arctic bloom . Scientific Reports , 9 ( 1 ): 8149 , https://doi.org/10.1038/s41598-019-44587-4 https://doi.org/10.1038/s41598-019-44587-4 .

Liu H B , Chen M R , Zhu F et al . 2016 . Effect of diatom silica content on copepod grazing, growth and reproduction. Frontiers in Marine Science , 3 : 89 , https://doi.org/10.3389/fmars.2016.00089 https://doi.org/10.3389/fmars.2016.00089 .

Losic D , Short K , Mitchell J G et al . 2007 . AFM nanoindentations of diatom biosilica surfaces . Langmuir , 23 ( 9 ): 5014 - 5021 , https://doi.org/10.1021/la062666y https://doi.org/10.1021/la062666y .

Ma J , Zhou B B , Duan D D et al . 2018 . Silicon limitation reduced the adsorption of cadmium in marine diatoms . Aquatic Toxicology , 202 : 136 - 144 , https://doi.org/10.1016/j.aquatox.2018.07.011 https://doi.org/10.1016/j.aquatox.2018.07.011 .

Martin-Jézéquel V , Hildebrand M , Brzezinski M A . 2000 . Silicon metabolism in diatoms: implications for growth . Journal of Phycology , 36 ( 5 ): 821 - 840 , https://doi.‍org/10.1046/j.1529-8817.2000.00019.x https://doi.‍org/10.1046/j.1529-8817.2000.00019.x . https://do 10.1046/j.1529-8817.2000.00019.x http://dx.doi.org/10.1046/j.1529-8817.2000.00019.x

McNair H M , Brzezinski M A , Krause J W . 2018 . Diatom populations in an upwelling environment decrease silica content to avoid growth limitation . Environmental Microbiology , 20 ( 11 ): 4184 - 4193 , https://doi.org/10.1111/1462-2920.14431 https://doi.org/10.1111/1462-2920.14431 .

Michels J , Gorb S N . 2015 . Mandibular gnathobases of marine planktonic copepods‍-‍feeding tools with complex micro-and nanoscale composite architectures . Beilstein Journal of Nanotechnology , 6 ( 1 ): 674 - 685 , https://doi.org/10.3762/bjnano.6.68 https://doi.org/10.3762/bjnano.6.68 .

Milligan A J , Morel F M M . 2002 . A proton buffering role for silica in diatoms . Science , 297 ( 5588 ): 1848 - 1850 , https://doi.org/10.1126/science.1074958 https://doi.org/10.1126/science.1074958 .

Moreno M D , Ma K K , Schoenung J et al . 2015 . An integrated approach for probing the structure and mechanical properties of diatoms: toward engineered nanotemplates . Acta Biomaterialia , 25 : 313 - 324 , https://doi.org/10.1016/j.actbio.2015.07.028 https://doi.org/10.1016/j.actbio.2015.07.028 .

Nelson D M , Riedel G F , Millan-Nunez R et al . 1984 . Silicon uptake by algae with no known Si requirement. I. True cellular uptake and pH-induced precipitation by phaeodactylum tricornutum (Bacillariophyceae) and Platymonas sp. (Prasinophyceae) . Journal of Phycology , 20 ( 1 ): 141 - 147 , https://doi.org/10.1111/j.0022-3646.1984.00141.x https://doi.org/10.1111/j.0022-3646.1984.00141.x .

Oliver W C , Pharr G M . 1992 . An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experiments . Journal of Materials Research , 7 ( 6 ): 1564 - 1583 , https://doi.org/10.1557/JMR.1992.1564 https://doi.org/10.1557/JMR.1992.1564

Onitsuka G , Shikata T , Kitatsuji S et al . 2016 . Factors influencing maintenance and decline of a diatom bloom in the Yatsushiro Sea, Japan . Journal of Oceanography , 72 ( 4 ): 617 - 627 , https://doi.‍org/10.1007/s10872-016-0358-0 https://doi.‍org/10.1007/s10872-016-0358-0 . https://do 10.1007/s10872-016-0358-0 http://dx.doi.org/10.1007/s10872-016-0358-0

Paasche E . 1973 . Silicon and the ecology of marine plankton diatoms. I. Thalassiosira pseudonana ( Cyclotella nana ) grown in a chemostat with silicate as limiting nutrient . Marine Biology , 19 ( 2 ): 117 - 126 , https://doi.‍org/10.1007/BF00353582 https://doi.‍org/10.1007/BF00353582 . https://do 10.1007/BF00353582 http://dx.doi.org/10.1007/BF00353582

Pančić M , Kiørboe T . 2018 . Phytoplankton defence mechanisms: traits and trade-offs . Biological Reviews , 93 ( 2 ): 1269 - 1303 , https://doi.org/10.1111/brv.12395 https://doi.org/10.1111/brv.12395 .

Pančić M , Torres R R , Almeda R et al . 2019 . Silicified cell walls as a defensive trait in diatoms . Proceedings of the Royal Society B : Biological Sciences , 286 ( 1901 ): 20190184 , https://doi.org/10.1098/rspb.2019.0184 https://doi.org/10.1098/rspb.2019.0184 .

Pondaven P , Gallinari M , Chollet S et al . 2007 . Grazing-induced changes in cell wall silicification in a marine diatom . Protist , 158 ( 1 ): 21 - 28 , https://doi.‍org/10.1016/j.protis.2006.09.002 https://doi.‍org/10.1016/j.protis.2006.09.002 . https://do 10.1016/j.protis.2006.09.002 http://dx.doi.org/10.1016/j.protis.2006.09.002

Ryderheim F , Grønning J , Kiørboe T . 2022 . Thicker shells reduce copepod grazing on diatoms . Limnology and Oceanography Letters , 7 ( 5 ): 435 - 442 , https://doi.org/10.1002/lol2.10243 https://doi.org/10.1002/lol2.10243 .

Sader J E , Borgani R , Gibson C T et al . 2016 . A virtual instrument to standardise the calibration of atomic force microscope cantilevers . Review of Scientific Instruments , 87 ( 9 ): 093711 , https://doi.org/10.1063/1.4962866 https://doi.org/10.1063/1.4962866 .

Schultz M , Kiørboe T . 2009 . Active prey selection in two pelagic copepods feeding on potentially toxic and non-toxic dinoflagellates . Journal of Plankton Research , 31 ( 5 ): 553 - 561 , https://doi.org/10.1093/plankt/fbp010 https://doi.org/10.1093/plankt/fbp010 .

Thangaraj S , Shang X M , Sun J et al . 2019 . Quantitative proteomic analysis reveals novel insights into intracellular silicate stress-responsive mechanisms in the diatom Skeletonema dohrnii . International Journal of Molecular Sciences , 20 ( 10 ): 2540 , https://doi.org/10.3390/ijms20102540 https://doi.org/10.3390/ijms20102540 .

Tréguer P , Bowler C , Moriceau B et al . 2018 . Influence of diatom diversity on the ocean biological carbon pump . Nature Geoscience , 11 ( 1 ): 27 - 37 , https://doi.org/10.1038/s41561-017-0028-x https://doi.org/10.1038/s41561-017-0028-x .

Vrieling E G , Gieskes W W C , Beelen T P M . 1999 . Silicon deposition in diatoms: control by the pH inside the silicon deposition vesicle . Journal of Phycology , 35 ( 3 ): 548 - 559 , https://doi.org/10.1046/j.‍1529-8817.1999.3530548.x https://doi.org/10.1046/j.‍1529-8817.1999.3530548.x .

Wang Y , Cai J , Jiang Y G et al . 2013 . Preparation of biosilica structures from frustules of diatoms and their applications: current state and perspectives . Applied Microbiology and Biotechnology , 97 ( 2 ): 453 - 460 , https://doi.org/10.1007/s00253-012-4568-0 https://doi.org/10.1007/s00253-012-4568-0 .

Xu H , Chen F Y , Zhang X D et al . 2024 . Grazer-induced changes on mechanical properties of diatoms frustule: a new proof for a watery arms race . Limnology and Oceanography Letters , 1 - 10 , https://doi.org/10.1002/lol2.10419. Accessed on 2024-07-01 https://doi.org/10.1002/lol2.10419.Accessedon2024-07-01 .

Xu H , Shi Z Y , Zhang X D et al . 2021 . Diatom frustules with different silica contents affect copepod grazing due to differences in the nanoscale mechanical properties . Limnology and Oceanography , 66 ( 9 ): 3408 - 3420 , https://doi.org/10.1002/lno.11887 https://doi.org/10.1002/lno.11887 .

Zhou B B , Ma J , Chen F Y et al . 2020 . Mechanisms underlying silicon-dependent metal tolerance in the marine diatom Phaeodactylum tricornutum . Environmental Pollution , 262 : 114331 , https://doi.org/10.1016/j.envpol.2020.114331 https://doi.org/10.1016/j.envpol.2020.114331 .

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