RNA interference with <italic style="font-style: italic">foxl2</italic> leads to upregulation of <italic style="font-style: italic">cyp19b</italic> and delayed development of female carp <italic style="font-style: italic">Cyprinus</italic> <italic style="font-style: italic">carpio</italic> var. <italic style="font-style: italic">koi</italic> gonads

Xiao MEI; Xinglei WANG; Qiang ZHOU; Bo LIU; Yishun WANG; Liuchang KONG; Shibo JIANG; Feng WANG

doi:10.1007/s00343-024-4113-x

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RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads

Biology | Updated：2025-05-27

- RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads
- RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads
- 海洋湖沼学报(英文) 2025年43卷第3期页码：954-966
- Affiliations：
  
  1.College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
  2.Qingdao Marine Science and Technology Museum, Qingdao 266003, China
  3.Shandong Tangwangli Agricultural Development Co., Ltd., Jinan 250106, China
- Author bio：
  
  randy966@163.com
- Funds：
  
  the Qingdao Aquarium Technology Collaborative Innovation Center Cooperation Project(20210021);the Researching Key Technologies for Selecting Excellent Koi Carp Germplasm(20223702032291);the Qingdao Agricultural University Tangwang Koi Carp Joint R&D Center Collaborative Project(20220271)
- DOI：10.1007/s00343-024-4113-x
  中图分类号：
- 收稿：2024-04-20，
  
  网络首发：2024-07-29，
  
  纸质出版：2025-05-01
- Accepted：
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RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads[J]. 海洋湖沼学报(英文), 2025,43(3):954-966.

MEI Xiao,WANG Xinglei,ZHOU Qiang,et al.RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads[J].Journal of Oceanology and Limnology,2025,43(03):954-966.
RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads[J]. 海洋湖沼学报(英文), 2025,43(3):954-966. DOI： 10.1007/s00343-024-4113-x.

MEI Xiao,WANG Xinglei,ZHOU Qiang,et al.RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads[J].Journal of Oceanology and Limnology,2025,43(03):954-966. DOI： 10.1007/s00343-024-4113-x.

摘要

Abstract

FOXL2 and CYP19B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development. The potential roles of the

foxl2

and

cyp19b

genes in sex determination and gonadal development in

Cyprinus

carpio

var.

koi

were explored using a non-invasive RNA interference (RNAi) method

histopathological observation and qPCR. Results demonstrate that

foxl2

exhibited a sexually dimorphic expression pattern in gonads

with a notable expression in ovaries;

cyp19b

was expressed in all peripheral tissues

with a particularly prominent expression in brain and gonads. The knockdown of

foxl2

by RNAi resulted in delay in the development of the female gonads. Conversely

no notable alterations were discerned in the gonads of

carpio

var.

koi

following the knockdown of

cyp19b

. The upregulation of

sox9a

amh

and

cyp19b

following

foxl

2 knockdown indicates that

foxl2

may play a pivotal role in gonadal development. Nevertheless

further investigation is required to ascertain the potential role of

cyp19b

. This study elucidated the role of

foxl2

and enhanced the understanding of the mechanisms of sex determination and gonadal development in

carpio

var.

koi

关键词

Keywords

references

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RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads

RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads

DOI：10.1007/s00343-024-4113-x

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Abstract

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