Novel insights into saline stress on photosynthetic activity and astaxanthin production of <italic style="font-style: italic">Haematococcus</italic> <italic style="font-style: italic">pluvialis</italic>

Cuili JIN; Jiajie YOU; Zepeng ZHOU; Qing LIU; Xiaojian ZHOU

doi:10.1007/s00343-024-4104-y

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Novel insights into saline stress on photosynthetic activity and astaxanthin production of Haematococcus pluvialis

Biology | Updated：2025-05-27

- Novel insights into saline stress on photosynthetic activity and astaxanthin production of Haematococcus pluvialis
- Novel insights into saline stress on photosynthetic activity and astaxanthin production of Haematococcus pluvialis
- 海洋湖沼学报(英文) 2025年43卷第3期页码：921-938
- Affiliations：
  
  1.College of Environmental Science & Engineering, Yangzhou University, Yangzhou 225009, China
  2.Marine Science & Technology Institute, Yangzhou University, Yangzhou 229009, China
- Author bio：
  
  zhouxiaojian@yzu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(42177459;41776156;41271521)
- DOI：10.1007/s00343-024-4104-y
  中图分类号：
- 收稿：2024-04-11，
  
  网络首发：2024-07-02，
  
  纸质出版：2025-05-01
- Accepted：
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Novel insights into saline stress on photosynthetic activity and astaxanthin production of Haematococcus pluvialis[J]. 海洋湖沼学报(英文), 2025,43(3):921-938.

JIN Cuili,YOU Jiajie,ZHOU Zepeng,et al.Novel insights into saline stress on photosynthetic activity and astaxanthin production of Haematococcus pluvialis[J].Journal of Oceanology and Limnology,2025,43(03):921-938.
Novel insights into saline stress on photosynthetic activity and astaxanthin production of Haematococcus pluvialis[J]. 海洋湖沼学报(英文), 2025,43(3):921-938. DOI： 10.1007/s00343-024-4104-y.

JIN Cuili,YOU Jiajie,ZHOU Zepeng,et al.Novel insights into saline stress on photosynthetic activity and astaxanthin production of Haematococcus pluvialis[J].Journal of Oceanology and Limnology,2025,43(03):921-938. DOI： 10.1007/s00343-024-4104-y.

摘要

Abstract

Saline treatment is a low-cost

simple

and effective method to stimulate astaxanthin accumulation in

Haematococcus

pluvialis

and is proposed to be applied in the second stage of a 2-stage culture since it does not necessitate changing the medium. To understand the effect of salinity on the astaxanthin production of

pluvialis

the photosynthetic activity and the biocomponents production in 1- and 2-stage cultures in different salinities were investigated. Except for astaxanthin synthesis

which increased at low salinities of 2 and 5-g/L NaCl

most biocomponent yields decreased in 1-stage cultures as salinity increased. At a salinity of 5-g/L NaCl

the 2-stage culture further increased astaxanthin production to 18.41±0.24 mg/L

which was more than 2.0 times that of the control. Saline treatment led to an overall decrease in photosynthetic performance indices of

pluvialis

and had an impact on five sites of the electron transport chain: the energy connection between antenna and reaction center of photosystem II (PS ‍II)

oxygen evolving complex activity on the donor side

the electron transfer from plastoquinone A (Q

) to plastoquinone B (Q

) and from plastoquinone (PQ) to receptor side of photosystem I (PS ‍I)

and the pool size of the end electron acceptors in PS ‍Ⅰ acceptor side. The excitation imbalance between PS Ⅰ and PS Ⅱ caused by the variance in the electron transfer chain necessitated the synthesis of antioxidants like astaxanthin in order to ensure cell viability. The accumulation of astaxanthin was found to be closely correlated with the stabilized or enhanced the maximum relative electron transfer rate (rETR

max

) and the PS ‍II actual quantum yield (QY

) as well as the increased fluore

scence yield at J-step (

). This work offers the novel insight of how saline stress controls

pluvialis

photosynthetic activity and astaxanthin synthesis.

关键词

Keywords

references

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Novel insights into saline stress on photosynthetic activity and astaxanthin production of Haematococcus pluvialis

DOI：10.1007/s00343-024-4104-y

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