Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp <italic style="font-style: italic">Artemia</italic> <italic style="font-style: italic">parthenogenetica</italic> through <italic style="font-style: italic">p53</italic> signaling pathway

Junfang WANG; Zhichao YAN; Yulong LU; Wei XIE; Liying SUI

doi:10.1007/s00343-024-4066-0

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Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway

Ecotoxicological implications for micro- and nano-plastics | Updated：2025-03-21

- Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway
- Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway
- 海洋湖沼学报(英文) 2025年43卷第2期页码：614-623
- Affiliations：
  
  1.Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China
  2.Key Laboratory of Marine Resource Chemistry and Food Technology (TUST), Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China
- Author bio：
  
  suily@tust.edu.cn
- Funds：
  
  the Program of Sustainable Development and Protection of Artemia Resources in Yuncheng Salt Lake of China(YHYJ-2023005)
- DOI：10.1007/s00343-024-4066-0
  中图分类号：
- 收稿：2024-03-04，
  
  网络首发：2024-06-03，
  
  纸质出版：2025-03-01
- Accepted：
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Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway[J]. 海洋湖沼学报(英文), 2025,43(2):614-623.

WANG Junfang,YAN Zhichao,LU Yulong,et al.Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway[J].Journal of Oceanology and Limnology,2025,43(02):614-623.
Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway[J]. 海洋湖沼学报(英文), 2025,43(2):614-623. DOI： 10.1007/s00343-024-4066-0.

WANG Junfang,YAN Zhichao,LU Yulong,et al.Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway[J].Journal of Oceanology and Limnology,2025,43(02):614-623. DOI： 10.1007/s00343-024-4066-0.

摘要

Abstract

Microplastics (MPs) are one of the most concerning pollutants that affects the health and growth of aquatic organisms. We characterized the MPs dispersion in the milli-Q water and seawater

and evaluated the effects of MPs on the gut epithelial cells of brine shrimp using three sizes of polystyrene (PS) microbeads (0.05

0.5

and 5 μm

respectively). Results show that microbeads evenly dispersed in milli-Q water

but exhibited aggregation tendency in seawater associating with the particle size. Apart from a reduced survival rate

we observed the structure changes in the gut epithelium that the smaller size of PS microbeads resulted in an increased reactive oxygen species (ROS) and higher apoptosis-related genes expression. Moreover

exposure to all size of PS microbeads led to increased green fluorescence of J-monomer

indicating the declined mitochondrial membrane potential. Therefore

exposure to PS microbeads led to significantly size-dependent toxicity on brine shrimp. Especially

0.05-μm PS microbeads were more toxic

leading to severe oxidative stress and activation of the

p53

Bax

Bcl2

pathway

ultimately resulting in cellular apoptosis and gut damage. These findings are important to understand the mechanism of MPs toxicity and its potential ecological risks to marine aquatic animals.

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Bachmann M , Möröy T . 2005 . The serine/threonine kinase Pim-1 . The International Journal of Biochemistry & Cell Biology , 37 ( 4 ): 726 - 730 , https://doi.‍org/10.‍1016/j.biocel.2004.11.005 https://doi.‍org/10.‍1016/j.biocel.2004.11.005 . https://do 10.1016/j.biocel.2004.11.005 http://dx.doi.org/10.1016/j.biocel.2004.11.005

Batel A , Linti F , Scherer M et al . 2016 . Transfer of benzo [a ] pyrene from microplastics to Artemia nauplii and further to zebrafish via a trophic food web experiment: CYP1A induction and visual tracking of persistent organic pollutants. Environmental Toxicology and Chemistry , 35 ( 7 ): 1656 - 1666 , https://doi.org/10.1002/etc.3361 https://doi.org/10.1002/etc.3361 .

Bergami E , Bocci E , Vannuccini M L et al . 2016 . Nano-sized polystyrene affects feeding, behavior and physiology of brine shrimp Artemia franciscana larvae . Ecotoxicology and Environmental Safety , 123 : 18 - 25 , https://doi.‍org/10.1016/j.ecoenv.2015.09.021 https://doi.‍org/10.1016/j.ecoenv.2015.09.021 . https://do 10.1016/j.ecoenv.2015.09.021 http://dx.doi.org/10.1016/j.ecoenv.2015.09.021

Besseling E , Wang B , Lürling M et al . 2014 . Nanoplastic affects growth of S . Obliquus and reproduction of D . magna . Environmental Science & Technology , 48 ( 20 ): 12336 - 12343 , https://doi.‍org/10.1021/es503001d https://doi.‍org/10.1021/es503001d . https://do 10.1021/es503001d http://dx.doi.org/10.1021/es503001d

Bhattacharya P , Lin S J , Turner J P et al . 2010 . Physical adsorption of charged plastic nanoparticles affects algal photosynthesis . The Journal of Physical Chemistry C , 114 ( 39 ): 16556 - 16561 , https://doi.org/10.1021/jp1054759 https://doi.org/10.1021/jp1054759 .

Bhuvaneshwari M , Thiagarajan V , Nemade P et al . 2018 . Toxicity and trophic transfer of P25 TiO 2 NPs from Dunaliella salina to Artemia salina : effect of dietary and waterborne exposure . Environmental Research , 160 : 39 - 46 , https://doi.org/10.1016/j.envres.2017.09.022 https://doi.org/10.1016/j.envres.2017.09.022 .

Chang X L , Kang M R , Shen Y H et al . 2021 . Bacillus coagulans SCC-19 maintains intestinal health in cadmium-exposed common carp ( Cyprinus carpio L.) by strengthening the gut barriers, relieving oxidative stress and modulating the intestinal microflora . Ecotoxicology and Environmental Safety , 228 : 112977 , https://doi.org/10.1016/j.ecoenv.2021.112977 https://doi.org/10.1016/j.ecoenv.2021.112977 .

Chen M S , Wu W W , Liu D W et al . 2021 . Evolution and structure of Api5 and its roles in anti-apoptosis . Protein & Peptide Letters , 28 ( 6 ): 612 - 622 , https://doi.org/10.2174/0929866527999201211195551 https://doi.org/10.2174/0929866527999201211195551 .

Cheng C H , Yang F F , Ling R Z et al . 2015 . Effects of ammonia exposure on apoptosis, oxidative stress and immune response in pufferfish ( Takifugu obscurus ) . Aquatic Toxicology , 164 : 61 - 71 , https://doi.‍org/10.1016/j.aquatox.2015.04.004 https://doi.‍org/10.1016/j.aquatox.2015.04.004 . https://do 10.1016/j.aquatox.2015.04.004 http://dx.doi.org/10.1016/j.aquatox.2015.04.004

Choi J H , Lee J H , Jo A H et al . 2023 . Microplastic polyamide toxicity: Neurotoxicity, stress indicators and immune responses in crucian carp, Carassius carassius . Ecotoxicology and Environmental Safety , 265 : 115469 , https://doi.org/10.1016/j.ecoenv.2023.115469 https://doi.org/10.1016/j.ecoenv.2023.115469 .

Cole M , Lindeque P , Halsband C et al . 2011 . Microplastics as contaminants in the marine environment: a review . Marine Pollution Bulletin , 62 ( 12 ): 2588 - 2597 , https://doi.org/10.1016/j.marpolbul.2011.09.025 https://doi.org/10.1016/j.marpolbul.2011.09.025 .

Gunasekara R A Y S A , Rekecki A , Cornillie P et al . 2011 . Morphological characteristics of the digestive tract of gnotobiotic Artemia franciscana nauplii . Aquaculture , 321 ( 1-2 ): 1 - 7 , https://doi.‍org/10.1016/j‍.aquaculture.2011.07.037 https://doi.‍org/10.1016/j‍.aquaculture.2011.07.037 . https://do 10.1016/j.aquaculture.2011.07.037 http://dx.doi.org/10.1016/j.aquaculture.2011.07.037

Jeong C B , Won E J , Kang H M et al . 2016 . Microplastic size-dependent toxicity, oxidative stress induction, and p-JNK and p-p38 activation in the monogonont rotifer ( Brachionus koreanus ) . Environmental Science & Technology , 50 ( 16 ): 8849 - 8857 , https://doi.‍org/10.‍1021/acs.est.6b01441 https://doi.‍org/10.‍1021/acs.est.6b01441 . https://do 10.1021/acs.est.6b01441 http://dx.doi.org/10.1021/acs.est.6b01441

Jiang N , Song P P , Li X X et al . 2022 . Dibutyl phthalate induced oxidative stress and genotoxicity on adult zebrafish ( Danio rerio ) brain . Journal of Hazardous Materials , 424 : 127749 , https://doi.org/10.1016/j.jhazmat.2021.127749 https://doi.org/10.1016/j.jhazmat.2021.127749 .

Kim L , Kim S A , Kim T H et al . 2021 . Synthetic and natural microfibers induce gut damage in the brine shrimp Artemia franciscana . Aquatic Toxicology , 232 : 105748 , https://doi.org/10.1016/j.aquatox.2021.105748 https://doi.org/10.1016/j.aquatox.2021.105748 .

Kögel T , Bjorøy Ø , Toto B et al . 2020 . Micro- and nanoplastic toxicity on aquatic life: determining factors . Science of The Total Environment , 709 : 136050 , https://doi.org/10.1016/j.scitotenv.2019.136050 https://doi.org/10.1016/j.scitotenv.2019.136050 .

Kokalj A J , Kunej U , Skalar T . 2018 . Screening study of four environmentally relevant microplastic pollutants: Uptake and effects on Daphnia magna and Artemia franciscana . Chemosphere , 208 : 522 - 529 , https://doi.org/10.1016/j.chemosphere.2018.05.172 https://doi.org/10.1016/j.chemosphere.2018.05.172 .

Lee J W , Kang H M , Won E J et al . 2016 . Multi-walled carbon nanotubes (MWCNTs) lead to growth retardation, antioxidant depletion, and activation of the ERK signaling pathway but decrease copper bioavailability in the monogonont rotifer ( Brachionus koreanus ) . Aquatic Toxicology , 172 : 67 - 79 , https://doi.org/10.1016/j.aquatox.2015.12.022 https://doi.org/10.1016/j.aquatox.2015.12.022 .

Liao J Z , Liu Y W , Yi J N et al . 2022 . Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis . Science of the Total Environment , 809 : 152188 , https://doi.org/10.1016/j.scitotenv.2021.152188 https://doi.org/10.1016/j.scitotenv.2021.152188 .

Makridis P , Vadstein O . 1999 . Food size selectivity of Artemia franciscana at three developmental stages . Journal of Plankton Research , 21 ( 11 ): 2191 - 2201 , https://doi.org/10.1093/plankt/21.11.2191 https://doi.org/10.1093/plankt/21.11.2191 .

MohanKumar S M J , Campbell A , Block M et al . 2008 . Particulate matter, oxidative stress and neurotoxicity . Neurotoxicology , 29 ( 3 ): 479 - 488 , https://doi.org/10.1016/j.neuro.2007.12.004 https://doi.org/10.1016/j.neuro.2007.12.004 .

Nayak S , Panesar P , Kumar H . 2009 . p53-Induced apoptosis and inhibitors of p53 . Current Medicinal Chemistry , 16 ( 21 ): 2627 - 2640 , https://doi.org/10.2174/092986709788681976 https://doi.org/10.2174/092986709788681976 .

Peixoto D , Amorim J , Pinheiro C et al . 2019 . Uptake and effects of different concentrations of spherical polymer microparticles on Artemia franciscana . Ecotoxicology and Environmental Safety , 176 : 211 - 218 , https://doi.‍org/10.1016/j.ecoenv.2019.03.100 https://doi.‍org/10.1016/j.ecoenv.2019.03.100 . https://do 10.1016/j.ecoenv.2019.03.100 http://dx.doi.org/10.1016/j.ecoenv.2019.03.100

Qiao R X , Deng Y F , Zhang S H et al . 2019a . Accumulation of different shapes of microplastics initiates intestinal injury and gut microbiota dysbiosis in the gut of zebrafish . Chemosphere , 236 : 124334 , https://doi.org/10.1016/j.chemosphere.2019.07.065 https://doi.org/10.1016/j.chemosphere.2019.07.065 .

Qiao R X , Sheng C , Lu Y F et al . 2019b . Microplastics induce intestinal inflammation, oxidative stress, and disorders of metabolome and microbiome in zebrafish . Science of the Total Environment , 662 : 246 - 253 , https://doi.org/10.1016/j.scitotenv.2019.01.245 https://doi.org/10.1016/j.scitotenv.2019.01.245 .

Serrão C , Marques-Santos L F . 2023 . The genus Artemia , the nanoplastics, the microplastics, and their toxic effects: a review . Environmental Science and Pollution Research , 30 ( 35 ): 83025 - 83050 , https://doi.org/10.1007/s11356-023-27868-4 https://doi.org/10.1007/s11356-023-27868-4 .

Shen J , Liu P , Sun Y Q et al . 2021 . Embryonic exposure to prothioconazole induces oxidative stress and apoptosis in zebrafish ( Danio rerio ) early life stage . Science of the Total Environment , 756 : 143859 , https://doi.‍org/10.1016/j.scitotenv.2020.143859 https://doi.‍org/10.1016/j.scitotenv.2020.143859 . https://do 10.1016/j.scitotenv.2020.143859 http://dx.doi.org/10.1016/j.scitotenv.2020.143859

Stapleton P A . 2019 . Toxicological considerations of nano-sized plastics . AIMS Environmental Science , 6 ( 5 ): 367 - 378 , https://doi.org/10.3934/environsci.2019.5.367 https://doi.org/10.3934/environsci.2019.5.367 .

Suman T Y , Jia P P , Li W G et al . 2020 . Acute and chronic effects of polystyrene microplastics on brine shrimp: first evidence highlighting the molecular mechanism through transcriptome analysis . Journal of Hazardous Materials , 400 : 123220 , https://doi.org/10.1016/j.‍jhazmat.2020.123220 https://doi.org/10.1016/j.‍jhazmat.2020.123220 .

Sun Q Y , Li Y , Shi L J et al . 2022 . Heavy metals induced mitochondrial dysfunction in animals: molecular mechanism of toxicity . Toxicology , 469 : 153136 , https://doi.org/10.1016/j.tox.2022.153136 https://doi.org/10.1016/j.tox.2022.153136 .

Vannuccini M L , Grassi G , Leaver M J et al . 2015 . Combination effects of nano-TiO 2 and 2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD) on biotransformation gene expression in the liver of European sea bass Dicentrarchus labrax . Comparative Biochemistry and Physiology Part C : Toxicology & Pharmacology , 176 - 177 : 71 - 78 , https://doi.org/10.1016/j.cbpc.2015.07.009 https://doi.org/10.1016/j.cbpc.2015.07.009 .

Varó I , Redón S , Garcia-Roger E M et al . 2015 . Aquatic pollution may favor the success of the invasive species A . franciscana . Aquatic Toxicology , 161 : 208 - 220 , https://doi.org/10.1016/j.aquatox.2015.02.008 https://doi.org/10.1016/j.aquatox.2015.02.008 .

Wang X L , Zhang J W , Lu C B et al . 2023 . Development toxicity and cytotoxicity of pyroxsulam on embryos and adults of zebrafish ( Danio rerio ) . Environmental Pollution , 319 : 121040 , https://doi.org/10.1016/j.envpol.2023.121040 https://doi.org/10.1016/j.envpol.2023.121040 .

Wang Y , Mao Z , Zhang M X et al . 2019a . The uptake and elimination of polystyrene microplastics by the brine shrimp, Artemia parthenogenetica , and its impact on its feeding behavior and intestinal histology . Chemosphere , 234 : 123 - 131 , https://doi.org/10.1016/j.chemosphere.2019.05.267 https://doi.org/10.1016/j.chemosphere.2019.05.267 .

Wang Y , Zhang D , Zhang M X et al . 2019b . Effects of ingested polystyrene microplastics on brine shrimp, Artemia parthenogenetica . Environmental Pollution , 244 : 715 - 722 , https://doi.org/10.1016/j.envpol.2018.10.024 https://doi.org/10.1016/j.envpol.2018.10.024 .

Zhang H Y , Ma X , Deng X et al . 2012 . PDCD10 interacts with STK25 to accelerate cell apoptosis under oxidative stress . Frontiers in Bioscience-Landmark , 17 ( 6 ): 2295 - 2305 , https://doi.org/10.2741/4053 https://doi.org/10.2741/4053 .

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Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway

Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway

DOI：10.1007/s00343-024-4066-0

摘要

Abstract

关键词

Keywords

references