A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from <italic style="font-style: italic">Lessonia</italic> <italic style="font-style: italic">trabeculata</italic> and their bioactivities

Changhui YAN; Mingxuan PAN; Lihua GENG; Quanbin ZHANG; Yadong HU; Jing WANG; Sujuan YE

doi:10.1007/s00343-024-4042-8

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A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities

Biology | Updated：2024-12-24

- A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities
- A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities
- 海洋湖沼学报(英文) 2024年42卷第6期页码：1998-2012
- Affiliations：
  
  1.College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao266061, China
  2.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao266071, China
  3.Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao266237, China
  4.Jiangsu Innovation Center of Marine Bioresources, Jiangsu Coast Development Investment Co., Ltd., Jiangsu Coast Development Group Co., Ltd., Nanjing210019, China
- Author bio：
  
  huyadong@jsyhkf.com
  jingwang@qdio.ac.cn
- Funds：
  
  the Jiangsu Coast Development Group Co., Ltd., the Marine Biological High Value Development and Utilization Project(2023YHTZZZ04);the Natural Science Foundation of Shandong Province(ZR2022MD007);the Science and Technology Project of Fujian Province(2022T3024;2023T3057)
- DOI：10.1007/s00343-024-4042-8
  中图分类号：
- 收稿：2024-02-06，
  
  纸质出版：2024-11-01
- Accepted：
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A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities[J]. 海洋湖沼学报(英文), 2024,42(6):1998-2012.

YAN Changhui,PAN Mingxuan,GENG Lihua,et al.Research Paper A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities[J].Journal of Oceanology and Limnology,2024,42(06):1998-2012.
A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities[J]. 海洋湖沼学报(英文), 2024,42(6):1998-2012. DOI： 10.1007/s00343-024-4042-8.

YAN Changhui,PAN Mingxuan,GENG Lihua,et al.Research Paper A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities[J].Journal of Oceanology and Limnology,2024,42(06):1998-2012. DOI： 10.1007/s00343-024-4042-8.

摘要

Abstract

Alginate oligosaccharides (AOS) and fucoidan are two important bioactive carbohydrate which that can be prepared from brown seaweeds. We investigated a novel enzyme-assisted one-pot method on AOS and fucoidan extraction from

Lessonia

trabeculata

and found that the optimum extraction procedure was: first

using 10-g/L Na

solution to digest the seaweeds for 2 h at 80 °‍‍C with the material-liquid ratio 1꞉20 (g/mL)

and then adding 4.0% fucoidan cleavage enzyme for reaction for 12 h at 45 °‍‍C. The yield of AOS and fucoidan was 21.36% and 14.40%

respectively

which was higher than those

using traditional methods. The degree of polymerization of AOS was determined to be 3–5

confirming the results through thin layer chromatography (TLC) and electrospray ionization mass spectrometry (ESI-MS). The ratio of guluronic acid to mannuronic acid in AOS was found to be 1.97. Through anion-exchange column chromatography

four sulfated polysaccharide fractions (F0.5

F1.0

F1.5

and F2.0) were successfully isolated from fucoidan. Chemical analysis revealed that fucoidan and F0.5 were heteropolysaccharides

consisting mainly of mannuronic acid

fucose

and xylose

while the other fractions were predominantly composed of fucose and galactose. The sulfate group content increased from 8.74% to 18.76%

while the uronic acid content decreased from 23.79% to 5.58% across the fractions. All fractions of fucoidan exhibited significant antioxidant activity in various tests. There was a positive correlation between the sulfate content and the ability to scavenge superoxide radicals. Additionally

both AOS and the fucoidan fractions showed immunostimulation activity and were non-toxic to RAW264.7 cells. These results indicate that the novel enzyme-assisted one-pot method is a green and efficient approach for the extraction of fucoidan and alginate oligosaccharides from brown seaweeds. It has potential applications in the processing industry of brown seaweeds.

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A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities

A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities

DOI：10.1007/s00343-024-4042-8

摘要

Abstract

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Keywords

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