Selection of protocols for phytoplankton pigment analysis: a comparative study

Jinxiu WANG; Fanzhou KONG; Zhuang NIU; Rencheng YU

doi:10.1007/s00343-024-4025-9

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Selection of protocols for phytoplankton pigment analysis: a comparative study

Ecology | Updated：2025-05-27

- Selection of protocols for phytoplankton pigment analysis: a comparative study
- Selection of protocols for phytoplankton pigment analysis: a comparative study
- 海洋湖沼学报(英文) 2025年43卷第3期页码：817-830
- Affiliations：
  
  1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao 266214, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
  4.Marine Monitoring and Forecasting Center of Zhejiang Province, Hangzhou 310007, China
- Author bio：
  
  fzkong@qdio.ac.cn
- Funds：
  
  the Joint Project of Guangxi Provincial and China National Natural Science Foundations(U20A20104;42306152);the Taishan Scholars Program to Prof. Rencheng YU
- DOI：10.1007/s00343-024-4025-9
  中图分类号：
- 收稿：2024-01-22，
  
  网络首发：2024-06-17，
  
  纸质出版：2025-05-01
- Accepted：
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Selection of protocols for phytoplankton pigment analysis: a comparative study[J]. 海洋湖沼学报(英文), 2025,43(3):817-830.

WANG Jinxiu,KONG Fanzhou,NIU Zhuang,et al.Selection of protocols for phytoplankton pigment analysis: a comparative study[J].Journal of Oceanology and Limnology,2025,43(03):817-830.
Selection of protocols for phytoplankton pigment analysis: a comparative study[J]. 海洋湖沼学报(英文), 2025,43(3):817-830. DOI： 10.1007/s00343-024-4025-9.

WANG Jinxiu,KONG Fanzhou,NIU Zhuang,et al.Selection of protocols for phytoplankton pigment analysis: a comparative study[J].Journal of Oceanology and Limnology,2025,43(03):817-830. DOI： 10.1007/s00343-024-4025-9.

摘要

Abstract

Pigments are widely used as indices for estimation of phytoplankton biomass and composition

and many protocols have been developed to analyze pigments in phytoplankton. Different protocols were compared using four solvents (methanol

95% methanol

dimethylformamide

and 90% acetone) and two instruments (fluorometer and high-performance liquid chromatography (HPLC) coupled with diode array detector). Analysis of chlorophyll

(Chl

) with fluorometer could lead to over- or underestimation due to the interference from its derivatives in all probability. Among the four extractants

90% acetone had a high recovery for chlorophylls. In contrast

95% methanol was a poor extractant for chlorophylls due to the degradation of Chl

especially in diatoms. The 95% methanol

however

had high extraction efficiencies for most diagnostic xanthophylls. Therefore

the selection of pigment analytical protocols should follow the specific purpose of phytoplankton study. In addition to fluorometry

an HPLC method with 90% acetone as extractant shall be a good choice for the analysis of Chl

to estimate phytoplankton biomass

especially for diatom-dominated samples

while an HPLC method with 95% methanol as extractant be more suitable to characterize different taxa in phytoplankton communities.

关键词

Keywords

references

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