Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding

Diwen LIANG; Chunrong HUANG; Senjie LIN; Jiahua DONG; Mingyi LIANG; Hailin LUO

doi:10.1007/s00343-024-3148-3

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Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding

Ecology | Updated：2024-11-19

- Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding
- Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding
- 海洋湖沼学报(英文) 2024年42卷第5期页码：1571-1585
- Affiliations：
  
  1.State Environmental Protection Key Laboratory of Urban Ecological Simulation and Protection, South China Institute of Environmental Sciences, Ministry of Ecology and Environment of the People’s Republic of China, Guangzhou510530, China
  2.Department of Marine Sciences, University of Connecticut, Groton, Connecticut06340, USA
- Author bio：
  
  luohailin@scies.org
- Funds：
  
  the Guangzhou Municipal Science and Technology Project(202201010592);the Fundamental Research Funds for the Central Public Welfare Research Institutes(PM-zx703-202305-165)
- DOI：10.1007/s00343-024-3148-3
  中图分类号：
- 收稿：2023-08-01，
  
  网络首发：2023-12-27，
  
  纸质出版：2024-09-01
- Accepted：
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Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding[J]. 海洋湖沼学报(英文), 2024,42(5):1571-1585.

LIANG Diwen,HUANG Chunrong,LIN Senjie,et al.Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding[J].Journal of Oceanology and Limnology,2024,42(05):1571-1585.
Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding[J]. 海洋湖沼学报(英文), 2024,42(5):1571-1585. DOI： 10.1007/s00343-024-3148-3.

LIANG Diwen,HUANG Chunrong,LIN Senjie,et al.Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding[J].Journal of Oceanology and Limnology,2024,42(05):1571-1585. DOI： 10.1007/s00343-024-3148-3.

摘要

Abstract

Zooplankton

a crucial component of urban wetland

are one of the effective bioindicators for monitoring the feeding stocks of organisms at higher trophic levels and assessing the ecological quality of ecosystems. However

information about the characteristics of epiphytic zooplankton community structure resulted from traditional methods is limited and hindered by the large amount of detritus and sludge attached to the macrophytes. We investigated the epiphytic zooplankton communities associated with macrophytes (

Vallisneria

Nymphaea

and

Thalia dealbata

) in a subtropical wetland using as DNA markers of the 18S rRNA gene and the mitochondrial cytochrome c oxidase subunit I (COI) gene. A total of 241 OTUs of zooplankton were obtained from COI amplicons

including 194 OTUs of Rotifera

22 of Cladocera

and 25 of Copepoda

while only 62 OTUs of zooplankton were obtained from 18S rDNA amplicons including 34 OTUs of Rotifera and 28 of Copepoda. The zooplankton communities associated with the three macrophytes were similar

but they differed significantly from those in the open waters. However

there were no significant temporal differences among the zooplankton communities. Epiphytic zooplankton communities were dominated by littoral zooplankton such as

Testudinella

Lecane

and

Philodina

. Microzooplankton

especially littoral species

utilize macrophytes as food sources and as refuges against predation. This further led to an increase in α and β diversity of zooplankton communities in urban wetlands. Our result suggests that the joint use of multiple molecular marke

rs could improve the taxonomic resolution and generate a comprehensive biodiversity profile of zooplankton.

关键词

Keywords

references

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