Heat stress induced hepatocyte apoptosis in largemouth bass <italic style="font-style: italic">Micropterus</italic> <italic style="font-style: italic">salmoides</italic> via IRE1α/TRAF2/ASK1/JNK pathway

Xuqian ZHAO; Wenjia MAO; Zijie LIN; Qufei LING

doi:10.1007/s00343-023-3094-5

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Heat stress induced hepatocyte apoptosis in largemouth bass Micropterus salmoides via IRE1α/TRAF2/ASK1/JNK pathway

Aquaculture and Fisheries | Updated：2026-05-18

- Heat stress induced hepatocyte apoptosis in largemouth bass Micropterus salmoides via IRE1α/TRAF2/ASK1/JNK pathway
- Heat stress induced hepatocyte apoptosis in largemouth bass Micropterus salmoides via IRE1α/TRAF2/ASK1/JNK pathway
- 海洋湖沼学报(英文) 2024年42卷第3期页码：988-1000
- Affiliations：
  
  School of Biology and Basic Medical Sciences, Suzhou Medical College of Soochow University, Suzhou 215000, China
- Author bio：
  
  lingqf@suda.edu.cn
- Funds：
  
  the Scientific Fund of Jiangsu Province(BY2015039-10);the Aquatic Three Project of Jiangsu Province(Y2017-37);the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Top-notch Academic Programs Project of Jiangsu Higher Education Institutions (TAPP), and the Jiangsu Funding Program for Excellent Postdoctoral Talent
- DOI：10.1007/s00343-023-3094-5
  中图分类号：
- 收稿：2023-05-23，
  
  录用：2023-07-06，
  
  网络首发：2023-08-23，
  
  纸质出版：2024-05-01
- Accepted：
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Xuqian ZHAO, Wenjia MAO, Zijie LIN, 等. Heat stress induced hepatocyte apoptosis in largemouth bass Micropterus salmoides via IRE1α/TRAF2/ASK1/JNK pathway[J]. 海洋湖沼学报(英文), 2024,42(3):988-1000. DOI: 10.1007/s00343-023-3094-5.

ZHAO Xuqian,MAO Wenjia,LIN Zijie,et al.Heat stress induced hepatocyte apoptosis in largemouth bass Micropterussalmoides via IRE1α/TRAF2/ASK1/JNK pathway[J].Journal of Oceanology and Limnology,2024,42(03):988-1000.
Xuqian ZHAO, Wenjia MAO, Zijie LIN, 等. Heat stress induced hepatocyte apoptosis in largemouth bass Micropterus salmoides via IRE1α/TRAF2/ASK1/JNK pathway[J]. 海洋湖沼学报(英文), 2024,42(3):988-1000. DOI: 10.1007/s00343-023-3094-5. DOI：

ZHAO Xuqian,MAO Wenjia,LIN Zijie,et al.Heat stress induced hepatocyte apoptosis in largemouth bass Micropterussalmoides via IRE1α/TRAF2/ASK1/JNK pathway[J].Journal of Oceanology and Limnology,2024,42(03):988-1000. DOI： 10.1007/s00343-023-3094-5.

摘要

Abstract

Heat stress (HS) has been shown to adversely affect fish

livers and can lead to extensive apoptosis. To investigate the relationship between endoplasmic reticulum (ER) stress and HS-induced apoptosis in fish livers

we isolated and cultured primary hepatocytes of largemouth bass

Micropterus

salmoides

by trypsin method

then established an in-vitro model of liver cells under HS (35 ℃‍). The contents of lactic dehydrogenase (LDH) and hydrogen peroxide (H

) were determined to evaluate the effects of HS on hepatocyte injury and oxidative stress. RT-qPCR was performed to discover the key genes in unfolded protein response (UPR) pathways involved at different HS duration. ERS inhibitor 4-PBA and IRE1α inhibitor 4μ8C were used to further investigate the effects of HS on IRE1α apoptosis pathway in hepatocytes. Results show that HS led to significant increases in the release of LDH

the content of H

and the expressions of oxidative protein folding genes (

ero1α

and

pdi

) under HS

suggesting severe hepatocyte injury and oxidative stress happened in heat-stressed largemouth bass hepatocytes

The continuous activation of IRE1α pathway genes (

grp78

grp94

atf6

perk

eif2a

atf4

chop

ire1α

traf2

ask1

jnk1

and

jnk2

) indicated that HS led significantly to ER stress. In particular

the mRNA expression levels of ER stress-related genes (

grp78

grp94

atf6

perk

ire1α

chop

jnk1

and

jnk2

) in the high temperature (HT) +4-PBA group and the HT+4μ8C group were significantly down-regulated under HS. After 4μ8C treatment

the expression levels of apoptosis-related genes (

caspase

and

caspase

) and LDH content were significantly decreased

whereas the cell survival rate was significantly increased when given 4-PBA or 4μ8C treatment. These findings demonstrate that HS could induce liver apoptosis of largemouth bass through the IRE1α pathway

which may act as a key switch mediating liver apoptosis of largemouth bass under HS.

关键词

Keywords

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Heat stress induced hepatocyte apoptosis in largemouth bass Micropterus salmoides via IRE1α/TRAF2/ASK1/JNK pathway

Heat stress induced hepatocyte apoptosis in largemouth bass Micropterus salmoides via IRE1α/TRAF2/ASK1/JNK pathway

DOI：10.1007/s00343-023-3094-5

摘要

Abstract

关键词

Keywords

references