Isolation, identification, and virulence gene analysis of pathogenic <italic style="font-style: italic">Aeromonas dhakensis</italic> in <italic style="font-style: italic">Macrobrachium rosenbergii</italic> and histopathological observation

Xin PENG; Haihui TU; Xinyi YAO; Xuan LAN; Zhenxiao ZHONG; Jinping LUO; Qiongying TANG; Shaokui YI; Zhenglong XIA; Guoliang YANG

doi:10.1007/s00343-023-3038-0

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Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation

Aquaculture and Fisheries | Updated：2024-10-11

- Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation
- Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation
- 海洋湖沼学报(英文) 2024年42卷第2期页码：664-675
- Affiliations：
  
  1.Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, China
  2.Jiangsu Shufeng Prawn Breeding Co., Ltd., Gaoyou 225654, China
- Author bio：
  
  tangqy@zjhu.edu.cn
  ygl0572@163.com
- Funds：
  
  the Earmarked Fund for the China Agriculture Research System(CARS-48);the Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties(2021C02069-4-3);the Major Research & Development Program (Modern Agriculture) of Jiangsu Province(BE2019352)
- DOI：10.1007/s00343-023-3038-0
  中图分类号：
- 收稿：2023-03-09，
  
  网络首发：2023-05-20，
  
  纸质出版：2024-03-01
- Accepted：
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Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation[J]. 海洋湖沼学报(英文), 2024,42(2):664-675.

PENG Xin,TU Haihui,YAO Xinyi,et al.Isolation, identification, and virulence gene analysis of pathogenic ,Aeromonas dhakensis in ,Macrobrachium rosenbergii and histopathological observation[J].Journal of Oceanology and Limnology,2024,42(02):664-675.
Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation[J]. 海洋湖沼学报(英文), 2024,42(2):664-675. DOI： 10.1007/s00343-023-3038-0.

PENG Xin,TU Haihui,YAO Xinyi,et al.Isolation, identification, and virulence gene analysis of pathogenic ,Aeromonas dhakensis in ,Macrobrachium rosenbergii and histopathological observation[J].Journal of Oceanology and Limnology,2024,42(02):664-675. DOI： 10.1007/s00343-023-3038-0.

摘要

Abstract

To identify the cause of mass mortality of adult

Macrobrachium

rosenbergii

in a farm in Gaoyou City

Jiangsu Province

China

a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying

rosenbergii

and identified as

Aeromonas

dhakensis

by purification culture

biochemical characterization

and 16S rRNA and

gyrB

gene sequence analysis. The results of the challenge test revealed that the strain was highly pathogenic and the 50% lethal dose (LD

) in 72 h to

rosenbergii

was 1.54×10

CFU/mL.

The amplification results of virulence genes show that strain DKQ-1 carried 9 virulence genes

including

ascV

aexT

aer

act

lip

ompAI

gcaT

acg

and

exu

supporting the strong virulence of strain DKQ-1 to

rosenbergii

. Histopathological observation of the hepatopancreas

gills

and intestines indicated that DKQ-1 injection into

rosenbergii

could cause serious tissue damage

which further supported the strong virulence of this strain. In addition

a drug susceptibility test revealed that strain DKQ-1 was sensitive to 16 kinds of antibiotics

resistant to 9 kinds of antibiotics

and had intermediate resistance to spectinomycin and kanamycin. This study is the first report of

dhakensis

isolated from

rosenbergii

and provided a reference for the pathogen identification of bacterial diseases in

rosenbergii

and for the prevention and treatment caused by

dhakensis

关键词

Keywords

references

Anabel A , Maria J F , Isabel I et al . 2008 . Analysis of 16S rRNA gene mutations in a subset of Aeromonas strains and their impact in species delineation . International Microbiology , 11 ( 3 ): 185 - 194 , https://doi.org/10.2436/20.1501.01.59 https://doi.org/10.2436/20.1501.01.59 .

Aravena-Román M , Inglis T J J , Henderson B et al . 2012 . Antimicrobial susceptibilities of Aeromonas strains isolated from clinical and environmental sources to 26 antimicrobial agents . Antimicrobial Agents and Chemotherapy , 56 ( 2 ): 1110 - 1112 , https://doi.org/10.1128/AAC.05387-11 https://doi.org/10.1128/AAC.05387-11 .

Beaz-Hidalgo R , Alperi A , Buján N et al . 2010 . Comparison of phenotypical and genetic identification of Aeromonas strains isolated from diseased fish . Systematic and Applied Microbiology , 33 ( 3 ): 149 - 153 , https://doi.org/10.1016/j.syapm.2010.02.002 https://doi.org/10.1016/j.syapm.2010.02.002 .

Beaz-Hidalgo R , Figueras M J . 2013 . Aeromonas spp. whole genomes and virulence factors implicated in fish disease . Journal of Fish Diseases , 36 ( 4 ): 371 - 388 , https://doi.org/10.1111/jfd.12025 https://doi.org/10.1111/jfd.12025 .

Beaz-Hidalgo R , Martínez-Murcia A , Figueras M J . 2013 . Reclassification of Aeromonas hydrophila subsp. dhakensis Huyset al. 2002 and Aeromonas aquariorum Martínez-Murciaet al. 2008 as Aeromonas dhakensis sp. nov. comb nov. and emendation of the species Aeromonas hydrophila . Systematic and Applied Microbiology , 36 ( 3 ): 171 - 176 , https://doi.org/org/10.1016/j.syapm.2012.12.007 https://doi.org/org/10.1016/j.syapm.2012.12.007 .

Behreans A L , Karber L . 1953 . Determination of LD 50 . Screening in Pharmacology . Academic Press , New York . 60 p.

Bergey D H , Holt J G . 1994 . Bergey's Manual of Determinative Bacteriology. 9 th edn . Williams & Wilkins, Baltimore .

Chacon M R , Soler L , Groisman E A et al . 2004 . Type III secretion system genes in c linical Aeromonas isolates . Journal of Clinical Microbiology , 42 ( 3 ): 1285 - 1287 , https://doi.org/10.1128/JCM.42.3.1285-1287.2004 https://doi.org/10.1128/JCM.42.3.1285-1287.2004 .

Chen P L , Tsai P J , Chen C S et al . 2015 . Aeromonas stool isolates from individuals with or without diarrhea in southern Taiwan: predominance of Aeromonas veronii . Journal of Microbiology, Immunology and Infection , 48 ( 6 ): 618 - 624 , https://doi.org/org/10.1016/j.jmii.2014.08.007 https://doi.org/org/10.1016/j.jmii.2014.08.007 .

Chen P L , Wu C J , Chen C S et al . 2014 . A comparative study of clinical Aeromonas dhakensis and Aeromonas hydrophila isolates in southern Taiwan: A . dhakensis is more predominant and virulent . Clinical Microbiology and Infection , 20 ( 7 ): O428 - O434 , https://doi.org/10.1111/1469-0691.12456 https://doi.org/10.1111/1469-0691.12456 .

Chopra A K , Houston C W . 1999 . Enterotoxins in Aeromonas -associated gastroenteritis . Microbes and Infection , 1 ( 13 ): 1129 - 1137 , https://doi.org/10.1016/s1286-4579(99)00202-6 https://doi.org/10.1016/s1286-4579(99)00202-6 .

Chopra A K , Xu X J , Ribardo D et al . 2000 . The cytotoxic enterotoxin of Aeromonas hydrophila induces proinflammatory cytokine production and activates arachidonic acid metabolism in Macrophages . Infection and Immunity , 68 ( 5 ): 2808 - 2818 , https://doi.org/10.1128/iai.68.5.2808-2818.2000 https://doi.org/10.1128/iai.68.5.2808-2818.2000 .

Codjoe F S , Donkor E S . 2017 . Carbapenem resistance: a review . Medical Sciences , 6 ( 1 ): 1 , https://doi.org/10.3390/medsci6010001 https://doi.org/10.3390/medsci6010001 .

Esteve C , Alcaide E , Blasco M D . 2012 . Aeromonas hydrophila subsp. dhakensis isolated from feces, water and fish in Mediterranean Spain . Microbes Environments , 27 ( 4 ): 367 - 373 , https://doi.org/10.1264/jsme2.ME12009 https://doi.org/10.1264/jsme2.ME12009 .

Eugenín F L L . 2015 . Aeromonas , un microorganismo ambiental de importancia en salud humana y animal . Universitat Rovira I Virgili, Tarragona, Spain , https://hdl.handle.net/10803/334686 https://hdl.handle.net/10803/334686 .

FAO . 2023 . Fishery and Aquaculture Statistics. Global production by production source 1950 - 2021 (FishStatJ) . In: FAO Fisheries and Aquaculture Division [online ] , Rome, Italy , https://www.fao.org/fishery/statistics/software/fishstatj/en https://www.fao.org/fishery/statistics/software/fishstatj/en .

Fernández-Bravo A , Figueras M J . 2020 . An update on the genus Aeromonas : taxonomy, epidemiology, and pathogenicity . Microorganisms , 8 ( 1 ): 129 , https://doi.org/10.3390/microorganisms8010129 https://doi.org/10.3390/microorganisms8010129 .

Gao X J , Miao Z , Li X X et al . 2019 . Pathogenicity of non-O1/ O139 Vibrio cholerae and its induced immune response in Macrobrachium rosenbergii . Fish & Shellfish Immunology , 92 : 300 - 307 , https://doi.org/10.1016/j.fsi.2019.06.032 https://doi.org/10.1016/j.fsi.2019.06.032 .

Gao X J , Zhou Y F , Zhu X H et al . 2021 . Enterobacter cloacae : a probable etiological agent associated with slow growth in the giant freshwater prawn Macrobrachium rosenbergii . Aquaculture , 530 : 735826 , https://doi.org/10.1016/j.aquaculture.2020.735826 https://doi.org/10.1016/j.aquaculture.2020.735826 .

Hoel S , Vadstein O , Jakobsen A N . 2017 . Species distribution and prevalence of putative virulence factors in mesophilic Aeromonas spp. isolated from fresh retail Sushi . Frontiers in Microbiology , 8 : 265738 , https://doi.org/10.3389/fmicb.2017.00931 https://doi.org/10.3389/fmicb.2017.00931 .

Huys G , Kämpfer P , Albert M J et al . 2002 . Aeromonas hydrophila subsp. dhakensis subsp. nov., isolated from children with diarrhoea in Bangladesh, and extended description of Aeromonas hydrophila subsp. hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980) . International Journal of Systematic and Evolutionary Microbiology , 52 ( 3 ): 705 - 712 , https://doi.org/10.1099/00207713-52-3-705 https://doi.org/10.1099/00207713-52-3-705 .

Jin S , Guan T , Hu M et al . 2022 . Isolation, identification and virulence gene characterization of Aeromonas dhakensis isolated from sea lion ( Zalophus californianus ) . Letters in Applied Microbiology , 74 ( 6 ): 932 - 940 , https://doi.org/10.1111/lam.13688 https://doi.org/10.1111/lam.13688 .

Küpfer M , Kuhnert P , Korczak B M et al . 2006 . Genetic relationships of Aeromonas strains inferred from 16S rRNA, gyrB and rpoB gene sequences . International Journal of Systematic and Evolutionary Microbiology , 56 ( Pt 12 ): 2743 - 2751 , https://doi.org/10.1099/ijs.0.63650-0 https://doi.org/10.1099/ijs.0.63650-0 .

Kitagawa H , Ohge H , Yu L et al . 2020 . Aeromonas dhakensis is not a rare cause of Aeromonas bacteremia in Hiroshima, Japan . Journal of Infection and Chemotherapy , 26 ( 2 ): 316 - 320 , https://doi.org/10.1016/j.jiac.2019.08.020 https://doi.org/10.1016/j.jiac.2019.08.020 .

Marchandin H , Teyssier C , De Buochberg M S et al . 2003 . Intra-chromosomal heterogeneity between the four 16S rRNA gene copies in the genus Veillonella: implications for phylogeny and taxonomy . Microbiology , 149 ( Pt 6 ): 1493 - 1501 , https://doi.org/10.1099/mic.0.26132-0 https://doi.org/10.1099/mic.0.26132-0 .

Martínez-Murcia A , Monera A , Alperi A et al . 2009 . Phylogenetic evidence suggests that strains of Aeromonas hydrophila subsp. dhakensis belong to the species Aeromonas aquariorum sp. nov . Current Microbiology , 58 ( 1 ): 76 - 80 , https://doi.org/10.1007/s00284-008-9278-6 https://doi.org/10.1007/s00284-008-9278-6 .

Martínez-Murcia A J , Saavedra M J , Mota V R et al . 2008 . Aeromonas aquariorum sp. nov., isolated from aquaria of ornamental fish . International Journal of Systematic and Evolutionary Microbiology , 58 ( Pt 5 ): 1169 - 1175 , https://doi.org/10.1099/ijs.0.65352-0 https://doi.org/10.1099/ijs.0.65352-0 .

Namba A , Mano N , Takano H et al . 2008 . OmpA is an adhesion factor of Aeromonas veronii , an optimistic pathogen that habituates in carp intesti nal tract . Journal of Applied Microbiology , 105 ( 5 ): 1441 - 1451 , https://doi.org/10.1111/j.1365-2672.2008.03883.x https://doi.org/10.1111/j.1365-2672.2008.03883.x .

Pérez L , Abarca M L , Latif-Eugenín F et al . 2015 . Aeromonas dhakensis pneumonia and sepsis in a neonate Risso's dolphin Grampus griseus from the Mediterranean Sea . Diseases of Aquatic Organisms , 116 ( 1 ): 69 - 74 , https://doi.org/10.3354/dao02899 https://doi.org/10.3354/dao02899 .

Polz M F , Cavanaugh C M . 1998 . Bias in template-to-product ratios in multitemplate PCR . Applied and Environmental Microbiology , 64 ( 10 ): 3724 - 3730 , https://doi.org/10.1128/aem.64.10.3724-3730.1998 https://doi.org/10.1128/aem.64.10.3724-3730.1998 .

Pu W , Guo G , Yang N et al . 2019 . Three species of Aeromonas ( A . dhakensis A hydrophila and A . jandaei ) isolated from freshwater crocodiles ( Crocodylus siamensis ) with pneumonia and septicemia . Letters in Applied Microbiology , 68 ( 3 ): 212 - 218 , https://doi.org/10.1111/lam.13112 https://doi.org/10.1111/lam.13112 .

Saurabh S , Sahoo P K . 2008 . Major diseases and the defence mechanism in giant freshwater prawn, Macrobrachium rosenbergii (de Man) . Proceedings of the National Academy of Sciences, India-Section B : Biological Sciences , 78 : 103 - 121 .

Sinclair H A , Heney C , Sidjabat H E et al . 2016 . Genotypic and phenotypic identification of Aeromonas species and CphA-mediated carbapenem resistance in Queensland, Australia . Diagnostic Microbiology and Infectious Disease , 85 ( 1 ): 98 - 101 , https://doi.org/10.1016/j.diagmicrobio.2016.02.005 https://doi.org/10.1016/j.diagmicrobio.2016.02.005 .

Sun J J , Zhang X J , Gao X J et al . 2016 . Characterization of Virulence Properties of Aeromonas veronii Isolated from Diseased Gibel Carp ( Carassius gibelio ) . International Journal of Molecular Sciences , 17 ( 4 ): 496 , https://doi.org/10.3390/ijms17040496 https://doi.org/10.3390/ijms17040496 .

Tomás J M . 2012 . The main Aeromonas pathogenic factors . International Scholarly Research Notices , 2012 : 256261 , https://doi.org/10.5402/2012/256261 https://doi.org/10.5402/2012/256261 .

Wu C J , Wang H C , Chen P L et al . 2013 . AQU-1, a chromosomal class C β-lactamase, among clinical Aeromonas dhakensis isolates: distribution and clinical significance . International Journal of Antimicrobial Agents , 42 ( 5 ): 456 - 461 , https://doi.org/10.1016/j.ijantimicag.2013.08.002 https://doi.org/10.1016/j.ijantimicag.2013.08.002 .

Yáñez M A , Catalán V , Apráiz D et al . 2003 . Phylogenetic analysis of members of the genus Aeromonas based on gyrB gene sequences . International Journal of Systematic and Evolutionary Microbiology , 53 ( Pt 3 ): 875 - 883 , https://doi.org/10.1099/ijs.0.02443-0 https://doi.org/10.1099/ijs.0.02443-0 .

Yamamoto S , Harayama S . 1995 . PCR Amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains . Applied and Environmental Microbiology , 61 ( 3 ): 1104 - 1109 , https://doi.org/10.1128/aem.61.3.1104-1109.1995 https://doi.org/10.1128/aem.61.3.1104-1109.1995 .

Zhao C Y , Wen H G , Huang S S et al . 2022 . A novel disease (water bubble disease) of the giant freshwater prawn macrobrachium rosenbergii caused by Citrobacter freundii : antibiotic treatment and effects on the antioxidant enzyme activity and immune responses . Antioxidants , 11 ( 8 ): 1491 , https://doi.org/10.3390/antiox11081491 https://doi.org/10.3390/antiox11081491 .

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Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation

Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation

DOI：10.1007/s00343-023-3038-0

摘要

Abstract

关键词

Keywords

references