Supplementation of alanine improves biomass accumulation and lipid production of <italic style="font-style: italic">Chlorella pyrenoidosa</italic> by increasing the respiratory and metabolic processes

Yongfu LI; Tianze ZHAO; Wei SUN; Ruiwen GAO; Guangyuan MA

doi:10.1007/s00343-023-3015-7

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Supplementation of alanine improves biomass accumulation and lipid production of Chlorella pyrenoidosa by increasing the respiratory and metabolic processes

Biology | Updated：2024-10-11

- Supplementation of alanine improves biomass accumulation and lipid production of Chlorella pyrenoidosa by increasing the respiratory and metabolic processes
- Supplementation of alanine improves biomass accumulation and lipid production of Chlorella pyrenoidosa by increasing the respiratory and metabolic processes
- 海洋湖沼学报(英文) 2024年42卷第2期页码：570-579
- Affiliations：
  
  1.Jiangsu Province Engineering Research Center for Marine Bioresources Sustainable Utilization, Key Laboratory of Marine Hazards Forecasting, Ministry of Natural Resources, Hohai University, Nanjing 210098, China
  2.College of Oceanography, Hohai University, Nanjing 210098, China
  3.Jiangsu Innovation Center of Marine Bioresources, Jiangsu Coast Development Group Co., Ltd., Nanjing 210019, China
- Author bio：
  
  liyongfu@hhu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(32002411;42276189);the Innovation and Entrepreneurship Project for College Students of Hohai University(2022102941027);the Jiangsu Innovation Center for Marine Bioresources(822153216)
- DOI：10.1007/s00343-023-3015-7
  中图分类号：
- 收稿：2023-01-25，
  
  网络首发：2023-04-06，
  
  纸质出版：2024-03-01
- Accepted：
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Supplementation of alanine improves biomass accumulation and lipid production of Chlorella pyrenoidosa by increasing the respiratory and metabolic processes[J]. 海洋湖沼学报(英文), 2024,42(2):570-579.

LI Yongfu,ZHAO Tianze,SUN Wei,et al.Supplementation of alanine improves biomass accumulation and lipid production of Chlorella pyrenoidosa by increasing the respiratory and metabolic processes[J].Journal of Oceanology and Limnology,2024,42(02):570-579.
Supplementation of alanine improves biomass accumulation and lipid production of Chlorella pyrenoidosa by increasing the respiratory and metabolic processes[J]. 海洋湖沼学报(英文), 2024,42(2):570-579. DOI： 10.1007/s00343-023-3015-7.

LI Yongfu,ZHAO Tianze,SUN Wei,et al.Supplementation of alanine improves biomass accumulation and lipid production of Chlorella pyrenoidosa by increasing the respiratory and metabolic processes[J].Journal of Oceanology and Limnology,2024,42(02):570-579. DOI： 10.1007/s00343-023-3015-7.

摘要

Abstract

The function of exogenous alanine (Ala) in regulating biomass accumulation

lipid production

photosynthesis

and respiration in

Chlorella

pyrenoidosa

was studied. Result shows that the supplementation of Ala increased

pyrenoidosa

biomass and lipid production in an 8-d batch culture. The concentration of 10 mmol/L of Ala was optimum and increased the microalgal cell biomass and lipid content by 39.3% and 21.4%

respectively

compared with that in the control (0-mmol/L Ala). Ala supplementation reduced photosynthetic activity while boosting respiratory activity and pyruvate levels

indicating that

pyrenoidosa

used exogenous Ala for biomass accumulation through the respiratory metabolic process. The accelerated respiratory metabolism due to Ala supplementation elevated the substrate pool and improved the lipogenic gene expression

promoting lipid production at last. This study provided a novel method for increasing biomass accumulation and lipid production and elucidated the role of Ala in regulating lipid production.

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references

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DOI：10.1007/s00343-023-3015-7

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