Characterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in <italic style="font-style: italic">Phascolosoma</italic> <italic style="font-style: italic">esculenta</italic>

Xinming GAO; Binbin FENG; Chen DU; Congcong HOU; Shan JIN; Daojun TANG; Junquan ZHU

doi:10.1007/s00343-023-2343-y

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Characterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta

Biology | Updated：2024-10-15

- Characterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta
- Characterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta
- 海洋湖沼学报(英文) 2024年42卷第1期页码：187-200
- Affiliations：
  
  1.Key Laboratory of Applied Marine Biotechnology by the Ministry of Education and Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315211, China
  2.College of Ecology, Lishui University, Lishui 323000, China
- Author bio：
  
  tangdaojun@nbu.edu.cn
  zhujunquan@nbu.edu.cn
- Funds：
  
  the Ningbo Science and Technology Plan Projects(2019B10016;2016C10004);the Major Science and Technology Projects in Zhejiang Province(2011C12013);the Natural Science Foundation of Zhejiang Province(LY18C190007);the Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, the K. C. Wong Magna Fund in Ningbo University
- DOI：10.1007/s00343-023-2343-y
  中图分类号：
- 收稿：2022-10-19，
  
  录用：2022-11-25，
  
  网络首发：2023-02-08，
  
  纸质出版：2024-01-03
- Accepted：
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Characterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta[J]. 海洋湖沼学报(英文), 2024,42(1):187-200.

GAO Xinming,FENG Binbin,DU Chen,et al.Research PaperCharacterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in ,Phascolosoma,esculenta[J].Journal of Oceanology and Limnology,2024,42(01):187-200.
Characterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta[J]. 海洋湖沼学报(英文), 2024,42(1):187-200. DOI： 10.1007/s00343-023-2343-y.

GAO Xinming,FENG Binbin,DU Chen,et al.Research PaperCharacterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in ,Phascolosoma,esculenta[J].Journal of Oceanology and Limnology,2024,42(01):187-200. DOI： 10.1007/s00343-023-2343-y.

摘要

Abstract

Mitochondria undergo morphological changes during spermatogenesis in some animals. The mechanism and role of mitochondrial morphology regulation

however

remain somewhat unclear. In this study

we analyzed the molecular characteristics

expression dynamics and subcellular localization of optic atrophy protein 1 (OPA1)

a mitochondrial fusion and cristae maintenance-related protein

to reveal the possible regulatory mechanisms underlying mitochondrial morphology in

Phascolosoma

esculenta

spermiogenesis. The full-length cDNA of the

esculenta

opa1

gene (

opa1

) is 3 743 bp in length and encodes 975 amino acids. The

-OPA1 protein is highly conservative and includes a transmembrane domain

a GTPase domain

two helical bundle domains

and a lipid-interacting stalk. Gene and protein expression was higher in the coelomic fluid (a site of spermatid development) of male

esculenta

and increased first and then decreased from March to December. Moreover

their expression during the breeding stage was significantl

y higher than during the non-breeding stage

suggesting that

-OPA1 is involved in

esculenta

reproduction. The

-OPA1 protein was more abundant in components consisting of many spermatids than in components without

indicating that

-OPA1 mainly plays a role in the spermatid in coelomic fluid. Moreover

-OPA1 was mainly detected in the spermatid mitochondria. Immunofluorescence experiments showed that the

-OPA1 are constitutively expressed and co-localized with mitochondria during spermiogenesis

suggesting its involvement in

esculenta

spermiogenesis. These results provide evidence for

-OPA1’s involvement in the regulation of mitochondrial morphology during spermiogenesis.

关键词

Keywords

references

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Characterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta

Characterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta

DOI：10.1007/s00343-023-2343-y

摘要

Abstract

关键词

Keywords

references