An efficient method for constructing a random insertional mutant library for forward genetics in <italic style="font-style: italic">Nannochloropsis</italic> <italic style="font-style: italic">oceanica</italic>

Zhongyi ZHANG; Hang LIU; Xiaohui PAN; Yanan ZONG; Leili FENG; Lixian LIU; Li GUO; Guanpin YANG

doi:10.1007/s00343-023-2332-1

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An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

Biology | Updated：2024-10-15

- An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica
- An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica
- 海洋湖沼学报(英文) 2024年42卷第1期页码：216-225
- Affiliations：
  
  1.College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
  2.Institutes of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China
  3.Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China
- Author bio：
  
  qdguoli@139.com
  yguanpin@ouc.edu.cn
- Funds：
  
  the National Key R&D Program of China(2018YFD0901506;2018YFD0900305);the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)(2018 SDKJ0406-3)
- DOI：10.1007/s00343-023-2332-1
  中图分类号：
- 收稿：2022-09-27，
  
  录用：2022-13-12，
  
  网络首发：2023-03-17，
  
  纸质出版：2024-01-03
- Accepted：
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An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica[J]. 海洋湖沼学报(英文), 2024,42(1):216-225.

ZHANG Zhongyi,LIU Hang,PAN Xiaohui,et al.Research Paper An efficient method for constructing a random insertional mutant library for forward genetics in ,Nannochloropsis,oceanica[J].Journal of Oceanology and Limnology,2024,42(01):216-225.
An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica[J]. 海洋湖沼学报(英文), 2024,42(1):216-225. DOI： 10.1007/s00343-023-2332-1.

ZHANG Zhongyi,LIU Hang,PAN Xiaohui,et al.Research Paper An efficient method for constructing a random insertional mutant library for forward genetics in ,Nannochloropsis,oceanica[J].Journal of Oceanology and Limnology,2024,42(01):216-225. DOI： 10.1007/s00343-023-2332-1.

摘要

Abstract

Insertional mutation

phenotypic evaluation

and mutated gene cloning are widely used to clone genes from scratch. Exogenous genes can be integrated into the genome during non-homologous end joining (NHEJ) of the double-strand breaks of DNA

causing insertional mutation. The random insertional mutant library constructed using this method has become a method of forward genetics for gene cloning. However

the establishment of a random insertional mutant library requires a high transformation efficiency of exogenous genes. Many microalgal species show a low transformation efficiency

making constructing random insertional mutant libraries difficult. In this study

we established a highly efficient transformation method for constructing a random insertional mutant library of

Nannochloropsis

oceanica

and tentatively tried to isolate its genes to prove the feasibility of the method. A gene that may control the growth rate and cell size was identified. This method will facilitate the genetic studies of

oceanica

which should also be a reference for other microalgal species.

关键词

Keywords

references

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An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

DOI：10.1007/s00343-023-2332-1

摘要

Abstract

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