Mapping the resting cysts of dinoflagellate <italic style="font-style: italic">Alexandrium catenella</italic> along the coast of Qinhuangdao, China

Wenjiao TANG; Huixia GENG; Yanjuan XI; Qingchun ZHANG; Xuexi TANG; Rencheng YU

doi:10.1007/s00343-022-2190-2

Your Location：

Home >

Browse articles >

Mapping the resting cysts of dinoflagellate Alexandrium catenella along the coast of Qinhuangdao, China

Continued from the back cover | Updated：2023-05-20

- Mapping the resting cysts of dinoflagellate Alexandrium catenella along the coast of Qinhuangdao, China
- Mapping the resting cysts of dinoflagellate Alexandrium catenella along the coast of Qinhuangdao, China
- 海洋湖沼学报（英文） 2022年40卷第6期页码：2312-2321
- Affiliations：
  
  1.College of Marine Life Science, Ocean University of China, Qingdao 266003, China
  2.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  3.Key Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
  4.University of Chinese Academy of Sciences, Beijing 100049, China
  5.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
  6.Hebei Academy of Ocean and Fishery Science, Qinhuangdao 066200, China
- Author bio：
  
  ZHANG Qingchun, E-mail: qczhang@qdio.ac.cn
  YU Rencheng, E-mail: rcyu@qdio.ac.cn
- Funds：
  
  the National S & T Basic Resources Investigation Program of China(2018YF100206);the National Key R & D Program of China(2019YFC1407901);the National Key R&D Program(2017YFC1600701);the Ministry of Science and Technology(MoST);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-022-2190-2
  中图分类号：
- 收稿：2022-04-14，
  
  录用：2022-4-29，
  
  网络首发：2022-06-29，
  
  纸质出版：2022-11
- Accepted：
Scan QR Code
Mapping the resting cysts of dinoflagellate Alexandrium catenella along the coast of Qinhuangdao, China[J]. 海洋湖沼学报（英文）, 2022,40(6):2312-2321.

Wenjiao TANG, Huixia GENG, Yanjuan XI, et al. Mapping the resting cysts of dinoflagellate Alexandrium catenella along the coast of Qinhuangdao, China[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2312-2321.
Mapping the resting cysts of dinoflagellate Alexandrium catenella along the coast of Qinhuangdao, China[J]. 海洋湖沼学报（英文）, 2022,40(6):2312-2321. DOI： 10.1007/s00343-022-2190-2.

Wenjiao TANG, Huixia GENG, Yanjuan XI, et al. Mapping the resting cysts of dinoflagellate Alexandrium catenella along the coast of Qinhuangdao, China[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2312-2321. DOI： 10.1007/s00343-022-2190-2.

摘要

Abstract

Dinoflagellate

Alexandrium catenella

is a cosmopolitan bloom-forming species with complex life cycle

the formation and germination of resting cysts are critical for its bloom dynamics. In the coastal waters of Qinhuangdao

A. catenella

has been identified as the major causative agent for paralytic shellfish poisoning

but there is little knowledge concerning its resting cysts in this region. In this study

three surveys were carried out along the coast of Qinhuangdao from 2020 to 2021 to map the distribution of

A. catenella

resting cysts

using a quantitative PCR (qPCR) assay specific for

A. catenella

. The resting cysts were detected in surface sediments during all the three surveys

and their distribution patterns were similar. High abundance of resting cysts (maximum 1 300 cysts/g sediment (wet weight)) were found in a region (119.62°E-119.99°E

39.67°N-39.98°N) northeast to the coastal waters of Qinhuangdao

where surface sediments were mainly composed of clay and silt (percentage above 50%). Prior to the formation of the

A. catenella

bloom in March 2021

the abundance of

A. catenella

vegetative cells in seawater had extremely significant positive correlation with the abundance of resting cysts in surface sediments

reflecting the important role of resting cysts in the initiation of

A. catenella

blooms. As far as we know

this is the first report on the distribution of

A. catenella

cysts along the coast of Qinhuangdao. The results will offer a sound basis for the future monitoring and mitigation of toxic

A. catenella

blooms and paralytic shellfish poisoning events in this region.

关键词

Keywords

references

Anderson D M, Alpermann T J, Cembella A D et al. 2012a. The globally distributed genus Alexandrium : multifaceted roles in marine ecosystems and impacts on human health. Harmful Algae , 14 : 10-35, https://doi.org/10.1016/j.hal.2011.10.012..

Anderson D M, Cembella A D, Hallegraeff G M. 2012b. Progress in understanding Harmful Algal Blooms: paradigm shifts and new technologies for research, monitoring, and management. Annual Review of Marine Science , 4 : 143-176, https://doi.org/10.1146/annurev-marine-120308-081121. .

Anderson D M, Fukuyo Y, Matsuoka K. 2003. Cyst methodologies. Manual on Harmful Marine Microalgae , 165-189.

Anderson D M, Keafer B A, Kleindinst J L et al. 2014. Alexandrium fundyense cysts in the Gulf of Maine: long-term time series of abundance and distribution, and linkages to past and future blooms. Deep Sea Research Part II : Topical Studies in Oceanography , 103 : 6-26, https://doi.org/10.1016/j.dsr2.2013.10.002. .

Anderson D M, Kulis D M, Sullivan J J et al. 1990. Dynamics and physiology of saxitoxin production by the dinoflagellates Alexandrium spp. Marine Biology , 104 (3): 511-524, https://doi.org/10.1007/BF01314358. .

Anderson D M, Morel F M M. 1979. The seeding of two red tide blooms by the germination of benthic Gonyaulax tamarensis hypnocysts. Estuarine and Coastal Marine Science , 8 (3): 279-293, https://doi.org/10.1016/0302-3524(79)90098-7. .

Anderson D M, Stock C A, Keafer B A et al. 2005. Alexandrium fundyense cyst dynamics in the Gulf of Maine. Deep Sea Research Part II : Topical Studies in Oceanography , 52 (19-21): 2522-2542, https://doi.org/10.1016/j.dsr2.2005.06.014. .

Anderson D M. 1998. Physiology and bloom dynamics of toxic Alexandrium species, with emphasis on life cycle transitions. Nato ASI Series G : Ecological Sciences , 41 : 29-48..

Asakawa M, Miyazawa K, Takayama H et al. 1995. Dinoflagellate Alexandrium tamarense as the source of paralytic shellfish poison (PSP) contained in bivalves from Hiroshima Bay, Hiroshima Prefecture, Japan. Toxicon , 33 (5): 691-697, https://doi.org/10.1016/0041-0101(94)00177-A..

Berdalet E, Fleming L E, Gowen R et al. 2016. Marine Harmful Algal Blooms, human health and wellbeing: challenges and opportunities in the 21st century. Journal of the Marine Biological Association of the United Kingdom , 96 (1): 61-91, https://doi.org/10.1017/S0025315415001733. .

Bolch C J S. 1997. The use of sodium polytungstate for the separation and concentration of living dinoflagellate cysts from marine sediments. Phycologia , 36 (6): 472-478, https://doi.org/10.2216/i0031-8884-36-6-472.1. .

Bravo I, Garcés E, Diogène J et al. 2006. Resting cysts of the toxigenic dinoflagellate genus Alexandrium in recent sediments from the Western Mediterranean coast, including the first description of cysts of A . kutnerae and A . peruvianum . European Journal of Phycology , 41 (3): 293-302, https://doi.org/10.1080/09670260600810360..

Brown A R, Lilley M, Shutler J et al. 2020. Assessing risks and mitigating impacts of harmful algal blooms on mariculture and marine fisheries. Reviews in Aquaculture , 12 (3): 1663-1688, https://doi.org/10.1111/raq.12403..

Chen H R. 2018. Emergency treatment and reflection of red tide event of Gymnodinium catenatum in Fujian sea area in 2017. Journal of Fisheries Research , 40 (4): 308-314, https://doi.org/10.14012/j.cnki.fjsc.2018.04.008. (in Chinese with English abstract). .

Christensen V G, Khan E. 2020. Freshwater neurotoxins and concerns for human, animal, and ecosystem health: a review of anatoxin- a and saxitoxin. Science of the Total Environment , 736 : 139515, https://doi.org/10.1016/j.scitotenv.2020.139515. .

Dai L, Yu R C, Geng H X et al. 2020. Resting cysts of Alexandrium catenella and A . pacificum (Dinophyceae) in the Bohai and Yellow Seas, China: abundance, distribution and implications for toxic algal blooms. Harmful Algae , 93 : 101794, https://doi.org/10.1016/j.hal.2020.101794. .

Ding L, Qiu J B, Li A F. 2017. Proposed biotransformation pathways for new metabolites of paralytic shellfish toxins based on field and experimental mussel samples. Journal of Agricultural and Food Chemistry , 65 (27): 5494-5502, https://doi.org/10.1021/acs.jafc.7b02101. .

Erdner D L, Percy L, Keafer B et al. 2010. A quantitative real-time PCR assay for the identification and enumeration of Alexandrium cysts in marine sediments. Deep Sea Research Part II : Topical Studies in Oceanography , 57 (3-4): 279-287, https://doi.org/10.1016/j.dsr2.2009.09.006. .

FAO (Food and Agriculture Organization of the United Nations). 2004. Marine Biotoxins. FAO, Rome. p. 5-52.

Folk R L, Andrews P B, Lewis D W. 1970. Detrital sedimentary rock classification and nomenclature for use in New Zealand. New Zealand Journal of Geology and Geophysics , 13 (4): 937-968, https://doi.org/10.1080/00288306.1970.10418211. .

Gao Y, Yu R C, Chen J H et al. 2015. Distribution of Alexandrium fundyense and A . pacificum (Dinophyceae) in the Yellow Sea and Bohai Sea. Marine Pollution Bulletin , 96 (1-2): 210-219, https://doi.org/10.1016/j.marpolbul.2015.05.025. .

Gao Y, Yu R C, Zhang Q C et al. 2013. Application of qPCR method in detection of Alexandrium tamarense species complex in China. Acta Scientiae Circumstantiae , 33 (8): 2256-2263, https://doi.org/10.13671/j.hjkxxb.2013.08.026. (in Chinese with English abstract).

Genovesi B, Berrebi P, Nagai S et al. 2015. Geographic structure evidenced in the toxic dinoflagellate Alexandrium pacificum Litaker ( A . catenella – group IV (Whedon & Kofoid) Balech) along Japanese and Chinese coastal waters. Marine Pollution Bulletin , 98 (1-2): 95-105, https://doi.org/10.1016/j.marpolbul.2015.07.009. .

Genovesi B, Laabir M, Masseret E et al. 2009. Dormancy and germination features in resting cysts of Alexandrium tamarense species complex (Dinophyceae) can facilitate bloom formation in a shallow lagoon (Thau, southern France). Journal of Plankton Research , 31 (10): 1209-1224, https://doi.org/10.1093/plankt/fbp066. .

Gu H F, Zeng N, Liu T T et al. 2013. Morphology, toxicity, and phylogeny of Alexandrium (Dinophyceae) species along the coast of China. Harmful Algae , 27 : 68-81, https://doi.org/10.1016/j.hal.2013.05.008. .

He R Y, McGillicuddy Jr D J, Keafer B A et al. 2008. Historic 2005 toxic bloom of Alexandrium fundyense in the western Gulf of Maine: 2. Coupled biophysical numerical modeling. Journal of Geophysical Research , 113 (C7): C07040, https://doi.org/10.1029/2007JC004602..

Jiang T J, Yin Y W, Huang W J et al. 2000. The characteristics of paralytic shellfish toxins in shellfish cultured in Daya Wan, Shenzhen. Journal of Jinan University ( Natural Science & Medicine Edition ), 21 (5): 65-69, https://doi.org/10.3969/j.issn.1000-9965.2000.05.013. (in Chinese with English abstract).

Kamikawa R, Nagai S, Hosoi-Tanabe S et al. 2007. Application of real-time PCR assay for detection and quantification of Alexandrium tamarense and Alexandrium catenella cysts from marine sediments. Harmful Algae , 6 (3): 413-420, https://doi.org/10.1016/j.hal.2006.12.004. .

Kim Y O, Choi J, Baek S H et al. 2020. Tracking Alexandrium catenella from seed-bed to bloom on the southern coast of Korea. Harmful Algae , 99 : 101922, https://doi.org/10.1016/j.hal.2020.101922. .

Kim Y O, Park M H, Han M S. 2002. Role of cyst germination in the bloom initiation of Alexandrium tamarense (Dinophyceae) in Masan Bay, Korea. Aquatic Microbial Ecology , 29 : 279-286, https://doi.org/10.3354/ame029279. .

Lassus P, Chomérat N, Hess P et al. 2016. Toxic and Harmful Microalgae of the World Ocean. International Society for the Study of Harmful Algae/Intergovernmental Oceanographic Commission of UNESCO, Denmark, p. 68.

Liang Y B, Li D M, Yao J Y et al. 2019. Progresses in investigation and research on phycotoxins and toxic microalgaes in the coastal waters of China. Oceanologia et Limnologia Sinica , 50 (3): 511-524. (in Chinese with English abstract).

Liu R Y, Liu L, Liang Y B et al. 2016. The distribution, impacts and risks of toxic microalgae and phycotoxins in China. Marine Environmental Science , 35 (5): 787-800, https://doi.org/10.13634/j.cnki.mes.2016.05.025. (in Chinese with English abstract).

Lundholm N, Ribeiro S, Andersen T J et al. 2011. Buried alive – germination of up to a century-old marine protist resting stages. Phycologia , 50 (6): 629-640, https://doi.org/10.2216/11-16.1. .

Luo D, Liu H. 2015. Numerical study on the tides and tidal currents in the bohai sea. Journal of Shanghai Ocean University , 24 (3): 457-464. (in Chinese with English abstract).

Miyazono A, Nagai S, Kudo I et al. 2012. Viability of Alexandrium tamarense cysts in the sediment of Funka Bay, Hokkaido, Japan: over a hundred year survival times for cysts. Harmful Algae , 16 : 81-88, https://doi.org/10.1016/j.hal.2012.02.001. .

Natsuike M, Yokoyama K, Nishitani G et al. 2017. Germination fluctuation of toxic Alexandrium fundyense and A . pacificum cysts and the relationship with bloom occurrences in Kesennuma Bay, Japan. Harmful Algae , 62 : 52-59, https://doi.org/10.1016/j.hal.2016.11.018. .

Persson A. 2000. Possible predation of cysts—a gap in the knowledge of dinoflagellate ecology? Journal of Plankton Research , 22 (4): 803-809, https://doi.org/10.1093/plankt/22.4.803. .

Ribeiro S, Berge T, Lundholm N et al. 2011. Phytoplankton growth after a century of dormancy illuminates past resilience to catastrophic darkness. Nature Communications , 2 : 311, https://doi.org/10.1038/ncomms1314. .

Richlen M L, Zielinski O, Holinde L et al. 2016. Distribution of Alexandrium fundyense (Dinophyceae) cysts in Greenland and Iceland, with an emphasis on viability and growth in the Arctic. Marine Ecology Progress Series , 547 : 33-46,https://doi.org/10.3354/meps11660. .

Shi Y J, Liu D Y, Shao H B et al. 2011. Distribution of dinoflagellate cysts in the surface sediments from the northern Yellow Sea, China. Marine Science Bulletin , 30 (3): 320-327, https://doi.org/10.3969/j.issn.1001-6392.2011.03.014. (in Chinese with English abstract).

Shimada H, Miyazono A. 2005. Horizontal distribution of toxic Alexandrium spp. (Dinophyceae) resting cysts around Hokkaido, Japan. Plankton Biology and Ecology , 52 (2): 76-84..

Shin H H, Li Z, Kim H J et al. 2021. Alexandrium catenella (Group I) and A . pacificum (Group IV) cyst germination, distribution, and toxicity in Jinhae-Masan Bay, Korea. Harmful Algae , 110 : 102122, https://doi.org/10.1016/j.hal.2021.102122. .

Shin H H, Lim D, Park S Y et al. 2013. Distribution of dinoflagellate cysts in Yellow Sea sediments. Acta Oceanologica Sinica , 32 (9): 91-98, https://doi.org/10.1007/s13131-013-0356-7. .

Tang W J, Lin Z R, Zhang Q C et al. 2022. An investigation on bloom dynamics of Alexandrium catenella and A . pacificum and toxin accumulation in shellfish along the coast of Qinhuangdao, China. Marine Pollution Bulletin , 183 : 114058, https://doi.org/10.1016/j.marpolbul.2022.114058..

Tang Y Z, Hu Z X, Deng Y Y. 2016. Characteristical life history (resting cyst) provides a mechanism for recurrence and geographic expansion of harmful algal blooms of dinoflagellates: a review. Studia Marina Sinica , (51): 132-154,https://doi.org/10.12036/hykxjk20160730001. (in Chinese with English abstract)

Tobin E D, Wallace C L, Crumpton C et al. 2019. Environmental drivers of paralytic shellfish toxin producing Alexandrium catenella blooms in a fjord system of northern Southeast Alaska. Harmful Algae , 88 : 101659, https://doi.org/10.1016/j.hal.2019.101659. .

Wang D Z, Zhang S F, Zhang Y et al. 2016. Paralytic shellfish toxin biosynthesis in cyanobacteria and dinoflagellates: a molecular overview. Journal of Proteomics , 135 : 132-140, https://doi.org/10.1016/j.jprot.2015.08.008. .

Winnepenninckx B, Backeljau T, De Wachter R. 1993. Extraction of high molecular weight DNA from molluscs. Trends in Genetics , 9 (12): 407, https://doi.org/10.1016/0168-9525(93)90102-N. .

Xia Y Z, Wang S S, Xin Q Y et al. 2010. Pollution survey of paralytic shellfish poison (PSP) from aquaculture zones of Changhai's sea area in Dalian. Food & Machinery , 26 (2): 54-56, https://doi.org/10.13652/j.issn.1003-5788.2010.02.025. (in Chinese with English abstract).

Yamaguchi M, Itakura S, Imai I et al. 1995. A rapid and precise technique for enumeration of resting cysts of Alexandrium spp. (Dinophyceae) in natural sediments. Phycologia , 34 (3): 207-214, https://doi.org/10.2216/i0031-8884-34-3-207.1. .

Yu R C, Lü S H, Qi Y Z et al. 2020. Progress and perspectives of harmful algal bloom studies in China. Oceanologia et Limnologia Sinica , 51 (4): 768-788, https://doi.org/10.11693/hyhz20200400127. (in Chinese with English abstract).

Yu R C, Zhang Q C, Liu Y et al. 2021. The dinoflagellate Alexandrium catenella producing only carbamate toxins may account for the seafood poisonings in Qinhuangdao, China. Harmful Algae , 103 : 101980, https://doi.org/10.1016/j.hal.2021.101980..

Zhang C, Yang T J. 2013. Distribution and variation law of mean tidal range in eastern coastal China. Port & Waterway Engineering , (1): 60-65, https://doi.org/10.16233/j.cnki.issn1002-4972.2013.01.001. (in Chinese with English abstract)

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Establishing an assessment framework for the risk of dinoflagellate HABs based on cyst-mapping of marine sediments

Seasonal dynamics of phytoplankton shapes the annual variation of community in the coastal waters of Qinhuangdao, a high-frequency HAB area in North China

Effects of modified clay on cysts of Scrippsiella trochoidea for harmful algal bloom control

Composition and distribution of dinoflagellate cysts in surficial sediments and their linkages to their free-living form in five bays of three eastern islands in the Philippines, western Pacific border

齿轮传动对转桨扇发动机总体性能建模

Mapping the resting cysts of dinoflagellate Alexandrium catenella along the coast of Qinhuangdao, China

Mapping the resting cysts of dinoflagellate Alexandrium catenella along the coast of Qinhuangdao, China

DOI：10.1007/s00343-022-2190-2

摘要

Abstract

关键词

Keywords

references