Comparative chloroplast genomes of <italic style="font-style: italic">Ulva prolifera</italic> and <italic style="font-style: italic">U. linza</italic> (Ulvophyceae) provide genetic resources for the development of interspecific markers

Wenzheng LIU; Qianchun LIU; Jin ZHAO; Xiu WEI; Peng JIANG

doi:10.1007/s00343-022-2045-x

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Comparative chloroplast genomes of Ulva prolifera and U. linza (Ulvophyceae) provide genetic resources for the development of interspecific markers

Marine harmful macroalgae | Updated：2023-05-20

- Comparative chloroplast genomes of Ulva prolifera and U. linza (Ulvophyceae) provide genetic resources for the development of interspecific markers
- Comparative chloroplast genomes of Ulva prolifera and U. linza (Ulvophyceae) provide genetic resources for the development of interspecific markers
- 海洋湖沼学报（英文） 2022年40卷第6期页码：2372-2384
- Affiliations：
  
  1.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
  4.College of Life Science, Qingdao University, Qingdao 266071, China
- Author bio：
  
  Peng JIANG, E-mail: jiangpeng@qdio.ac.cn
- Funds：
  
  the Chinese National Projects, the Science and Technology Basic Resources Investigation Program of China(2018FY100205);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA23050302);the National Key R&D Program of China(2018YFD0901500);the National Natural Science Foundation of China(41776153);the Key R&D Program of Shandong Province(2019GSF107012);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022Electronic supplementary materialSupplementary material (Supplementary Tables S1–S3 and Figs.S1–S3) is available in the online version of this article at https://doi.org/10.1007/s00343-022-2045-x.
- DOI：10.1007/s00343-022-2045-x
  中图分类号：
- 收稿：2022-01-29，
  
  录用：2022-4-2，
  
  网络首发：2022-05-09，
  
  纸质出版：2022-11
- Accepted：
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Comparative chloroplast genomes of Ulva prolifera and U. linza (Ulvophyceae) provide genetic resources for the development of interspecific markers[J]. 海洋湖沼学报（英文）, 2022,40(6):2372-2384.

Wenzheng LIU, Qianchun LIU, Jin ZHAO, et al. Comparative chloroplast genomes of Ulva prolifera and U. linza (Ulvophyceae) provide genetic resources for the development of interspecific markers[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2372-2384.
Comparative chloroplast genomes of Ulva prolifera and U. linza (Ulvophyceae) provide genetic resources for the development of interspecific markers[J]. 海洋湖沼学报（英文）, 2022,40(6):2372-2384. DOI： 10.1007/s00343-022-2045-x.

Wenzheng LIU, Qianchun LIU, Jin ZHAO, et al. Comparative chloroplast genomes of Ulva prolifera and U. linza (Ulvophyceae) provide genetic resources for the development of interspecific markers[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2372-2384. DOI： 10.1007/s00343-022-2045-x.

摘要

Abstract

The green seaweeds

Ulva linza

and

U. prolifera

are closely related species. They usually co-occur widely and have important ecological significance as primary producers thriving in the intertidal zone. In the Yellow Sea

a genetically unique floating ecotype of

U. prolifera

even bloomed to cause serious green tides. However

there is still a lack of appropriate molecular markers to distinguish these two species

partially due to limited evaluations on the intraspecific variations in

U. prolifera

among different ecotypes. Since organelle genomes could provide rich genetic resources for phylogenetic analysis and development of genetic markers

in this study

the chloroplast genome from one attached population of

U. prolifera

was completely sequenced

and comparative genomic analyses were performed with other existing chloroplast genomes from

U. linza

and the floating ecotype of

U. prolifera

. The results showed that in spite of the high level of collinearity among three genomes

there were plenty of genetic variations especially within the non-coding regions

including introns and gene spacer regions. A strategy was proposed that only those signals of variation

which were identical between two ecotypes of

U. prolifera

but divergent between

U. linza

and

U. prolifera

were selected to develop the interspecific markers for

U. linza

and

U. prolifera

. Two candidate markers

psa

B and

pet

were shown to be able to distinguish these two closely related species and were applicable to more attached populations of

U. prolifera

from a wide range of geographical sources. In addition to the interspecific marker

this study would also provide resources for the development of intraspecific markers for

U. prolifera

. These markers might contribute to the surveys for

Ulva

species composition and green tide monitoring especially in the Yellow Sea region.

关键词

Keywords

references

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DOI：10.1007/s00343-022-2045-x

摘要

Abstract

关键词

Keywords

references