Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder <italic style="font-style: italic">Paralichthys olivaceus</italic>

Shuang JIAO; Xungang TAN; Feng YOU; Shujing ZHANG; Qiuxiang PANG

doi:10.1007/s00343-022-1358-0

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Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus

Biology | Updated：2023-04-27

- Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus
- Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus
- 海洋湖沼学报（英文） 2023年41卷第1期页码：280-289
- Affiliations：
  
  1.Anti-Aging & Regenerative Medicine Research Institution, School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255000, China
  2.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  3.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
- Author bio：
  
  Shuang JIAO, E-mail: jiaoshuang@sdut.edu.cn
  Xungang TAN, E-mail: tanx@qdio.ac.cn
- Funds：
  
  the National Natural Science Foundation of China(31972774);the Key Research and Development Program of Shandong Province, China(2019GHY112007);the National Natural Science Foundation of China(31672636);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2023
- DOI：10.1007/s00343-022-1358-0
  中图分类号：
- 收稿：2021-10-29，
  
  录用：2021-12-6，
  
  网络首发：2022-01-07，
  
  纸质出版：2023-01
- Accepted：
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Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus[J]. 海洋湖沼学报（英文）, 2023,41(1):280-289.

Shuang JIAO, Xungang TAN, Feng YOU, et al. Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus[J]. Journal of Oceanology and Limnology, 2023, 41(1): 280-289.
Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus[J]. 海洋湖沼学报（英文）, 2023,41(1):280-289. DOI： 10.1007/s00343-022-1358-0.

Shuang JIAO, Xungang TAN, Feng YOU, et al. Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus[J]. Journal of Oceanology and Limnology, 2023, 41(1): 280-289. DOI： 10.1007/s00343-022-1358-0.

摘要

Abstract

Insulin-like growth factors (IGFs) are key regulators of development and growth. Here

we characterized the

igf2

gene from olive flounder (

Paralichthys olivaceus

) and determined its temporal and spatial expression. We set up an in-vitro protein expression system in eukaryotic human embryonic kidney (HEK293T) cells and explored its effects on cell proliferation. The flounder

igf2

cDNA contained a 648-bp open reading frame (ORF) encoding a protein of 215 amino acids (aa)

which spanned the complete signal peptide (47 aa)

mature peptide (70 aa)

and E domain (98 aa). In adult flounder

igf2

mRNA was detected in all selected tissues. In early development

igf2

mRNA was detected throughout development from unfertilized eggs to hatching-stage embryos. In-situ hybridization analysis indicated that

igf2

mRNA was specially expressed in the brain region

floor plate

hypochord

otic vesicle

and pectoral fin during embryogenesis. Western blotting analysis indicated that the soluble recombinant flounder IGF2 protein was successfully produced through eukaryotic expression in HEK293T cells. In addition

the recombinant IGF2 protein significantly promoted the proliferation of human cervical carcinoma (HeLa) and HEK293T cells. These results provide new information about the structural and functional conservation

expression patterns

and biological activity of the

igf2

in teleosts.

关键词

Keywords

references

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Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus

Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus

DOI：10.1007/s00343-022-1358-0

摘要

Abstract

关键词

Keywords

references