Protective efficacy evaluation of immunogenic protein AHA_3793 of <italic style="font-style: italic">Aeromonas hydrophila</italic> as vaccine candidate for largemouth bass <italic style="font-style: italic">Micropterus salmoides</italic>

Shun YANG; Idefonce MKINGULE; Long LIU; Wenqi CHEN; Xiangyu YUAN; Zixuan MA; Liang LIANG; Shichao QIAN; Mengmeng HUANG; Hui FEI

doi:10.1007/s00343-022-1326-8

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Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides

Aquaculture and Fisheries | Updated：2023-04-27

- Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides
- Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides
- 海洋湖沼学报（英文） 2023年41卷第1期页码：392-400
- Affiliations：
  
  1.College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
  2.Zhejiang Development&Planning Institute, Hangzhou 310012, China
  3.Huzhou Baijiayu Biotech Co., Ltd., Huzhou 313000, China
- Author bio：
  
  Hui FEI, E-mail: feihui@zstu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(32102824);the Zhejiang Provincial Natural Science Foundation of China(LQ18B060005);the Zhejiang Sci-Tech University Foundation(11612932611702);the Zhejiang Sci-Tech University Foundation(11612832611909);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2023Electronic supplementary material Supplementary material (Supplementary Figs.S1–S2) is available in the online version of this article at https://doi.org/10.1007/s00343-022-1326-8.
- DOI：10.1007/s00343-022-1326-8
  中图分类号：
- 收稿：2021-10-12，
  
  录用：2021-12-13，
  
  网络首发：2022-01-13，
  
  纸质出版：2023-01
- Accepted：
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Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides[J]. 海洋湖沼学报（英文）, 2023,41(1):392-400.

Shun YANG, Idefonce MKINGULE, Long LIU, et al. Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides[J]. Journal of Oceanology and Limnology, 2023, 41(1): 392-400.
Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides[J]. 海洋湖沼学报（英文）, 2023,41(1):392-400. DOI： 10.1007/s00343-022-1326-8.

Shun YANG, Idefonce MKINGULE, Long LIU, et al. Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides[J]. Journal of Oceanology and Limnology, 2023, 41(1): 392-400. DOI： 10.1007/s00343-022-1326-8.

摘要

Abstract

Aeromonas hydrophila

is a Gram-negative pathogen that can infect various fish

including largemouth bass (

Micropterus salmoides

)

which have caused huge economic losses. In present study

largemouth bass anti-

hydrophila

antibodies were produced

then a highly immunogenic outer membrane proteins

AHA_3793

was identified by combined western blotting and mass spectrometry analysis. Moreover

AHA_3793 was expressed

and its immunogenicity was further verified by western blotting. Subsequently

the protective efficacy of AHA_3793 were evaluated in largemouth bass. The results showed that rAHA_3793 could produce a relative percentage survival (RPS) of 61.76% for largemouth bass against

hydrophila

challenge. ELISA analysis showed the specific serum antibodies of largemouth bass against rAHA_3793 and

hydrophila

in vaccinated group in weeks 4 and 5 after immunization were significantly higher than those in control group

which suggested that rAHA_3793 induced production of specific serum antibodies against rAHA_3793 and

hydrophila

. The qRT-PCR analysis showed that expressions of

CD4

and

MHC IIα

were also significantly up-regulated after immunization. These results collectively demonstrated that rAHA_3793 could induce a strong humoral immune response of largemouth bass

and then produce high immune protection effects against

hydrophila

infection.

关键词

Keywords

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Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides

Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides

DOI：10.1007/s00343-022-1326-8

摘要

Abstract

关键词

Keywords

references