Physiological and molecular responses of invasive cyanobacterium<italic style="font-style: italic">Raphidiopsis raciborskii</italic>to ambient phosphorus deficiency

Junqiong SHI; Shuhan HE; Lu ZHAO; Lulu JI; Songqi YANG; Zhongxing WU

doi:10.1007/s00343-022-1314-z

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Physiological and molecular responses of invasive cyanobacteriumRaphidiopsis raciborskiito ambient phosphorus deficiency

Special Section: Cyanobacterial blooms in China: ecology, toxicity, and treatment | Updated：2023-05-19

- Physiological and molecular responses of invasive cyanobacteriumRaphidiopsis raciborskiito ambient phosphorus deficiency
- Physiological and molecular responses of invasive cyanobacteriumRaphidiopsis raciborskiito ambient phosphorus deficiency
- 海洋湖沼学报（英文） 2022年40卷第5期页码：1792-1803
- Affiliations：
  
  Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing 400715, China
- Author bio：
  
  Zhongxing WU,wuzhx@swu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(42177055);the National Natural Science Foundation of China(41877410);the Chongqing Postgraduate Scientific Research Innovation Project(CYS21106);the Chongqing Postgraduate Scientific Research Innovation Project(CYS20105);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-022-1314-z
  中图分类号：
- 收稿：2021-10-09，
  
  录用：2021-11-19，
  
  网络首发：2022-01-18，
  
  纸质出版：2022-09
- Accepted：
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Physiological and molecular responses of invasive cyanobacteriumRaphidiopsis raciborskiito ambient phosphorus deficiency[J]. 海洋湖沼学报（英文）, 2022,40(5):1792-1803.

Junqiong SHI, Shuhan HE, Lu ZHAO, et al. Physiological and molecular responses of invasive cyanobacteriumRaphidiopsis raciborskiito ambient phosphorus deficiency[J]. Journal of Oceanology and Limnology, 2022, 40(5): 1792-1803.
Physiological and molecular responses of invasive cyanobacteriumRaphidiopsis raciborskiito ambient phosphorus deficiency[J]. 海洋湖沼学报（英文）, 2022,40(5):1792-1803. DOI： 10.1007/s00343-022-1314-z.

Junqiong SHI, Shuhan HE, Lu ZHAO, et al. Physiological and molecular responses of invasive cyanobacteriumRaphidiopsis raciborskiito ambient phosphorus deficiency[J]. Journal of Oceanology and Limnology, 2022, 40(5): 1792-1803. DOI： 10.1007/s00343-022-1314-z.

摘要

Abstract

Raphidiopsis raciborskii

can cause harmful cyanobacterial blooms when concentrations of environmental phosphorus (P) are very low

thus the physiological and molecular mechanisms involved in the acclimation to P need to be characterized better. The growth

chlorophyll fluorescence

alkaline phosphatase

and expression of genes directly involved in P assimilation were compared in the

R. raciborskii

FACHB 1496 strain grown with and without inorganic P. The specific growth rate (

)

Chl

and six fluorescence parameters (minimal fluorescence (

)

maximal fluorescence (

)

maximal variable fluorescence (

)

electron transport flux (further than

) per RC (ET

/RC)

quantum yield of the electron transport in PSII (

)

and the probability that an electron from a trapped exciton is moved into the electron transport chain beyond

−

(

)) markedly decreased in

R. raciborskii

in response to experimental P-deficiency. In contrast

the relative variable fluorescence at the J-step (

)

trapped energy flux (leading to

reduction) per RC (TR

/RC)

and alkaline phosphatase activity significantly increased. In addition

gene expressions involved in the alkaline phosphatase (

phoA1

and

phoA2

)

high-affinity inorganic P transporter (

pstS1

)

phosphonate transporter and metabolism (

phnD

and

phnM

)

and nucleotidase (

nucH

) were significantly upregulated under P deficiency. However

physiological and molecular responses were resumed rapidly after P re-supplementation following P-deficient conditions. Our results highlight that

R. raciborskii

can perform coordinated and complex cellular and physiological responses to cope with P deficiency

reflecting

R. raciborskii'

s multi-faceted machinery to respond to environmental P fluctuations

关键词

Keywords

references

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Physiological and molecular responses of invasive cyanobacteriumRaphidiopsis raciborskiito ambient phosphorus deficiency

DOI：10.1007/s00343-022-1314-z

摘要

Abstract

关键词

Keywords

references