Sodium acetate can promote the growth and astaxanthin accumulation in the unicellular green alga<italic style="font-style: italic">Haematococcus pluvialis</italic>as revealed by a proteomics approach

Xinlei YU; Xin YE; Chaoyang HU; Nianjun XU; Xue SUN

doi:10.1007/s00343-021-1271-y

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Sodium acetate can promote the growth and astaxanthin accumulation in the unicellular green algaHaematococcus pluvialisas revealed by a proteomics approach

Biology | Updated：2023-05-19

- Sodium acetate can promote the growth and astaxanthin accumulation in the unicellular green algaHaematococcus pluvialisas revealed by a proteomics approach
- Sodium acetate can promote the growth and astaxanthin accumulation in the unicellular green algaHaematococcus pluvialisas revealed by a proteomics approach
- 海洋湖沼学报（英文） 2022年40卷第5期页码：2052-2067
- Affiliations：
  
  Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315211, China
- Author bio：
  
  Xue SUN,sunxue@nbu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(31572638);the K. C. Wong Magna Fund in Ningbo University;© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022Electronic supplementary materialSupplementary material (Supplementary Tables S1–S2 and Figs.S1–S3) is available in the online version of this article athttps://doi.org/10.1007/s00343-021-1271-y.
- DOI：10.1007/s00343-021-1271-y
  中图分类号：
- 收稿：2021-08-18，
  
  录用：2021-11-3，
  
  网络首发：2021-11-22，
  
  纸质出版：2022-09
- Accepted：
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Sodium acetate can promote the growth and astaxanthin accumulation in the unicellular green algaHaematococcus pluvialisas revealed by a proteomics approach[J]. 海洋湖沼学报（英文）, 2022,40(5):2052-2067.

Xinlei YU, Xin YE, Chaoyang HU, et al. Sodium acetate can promote the growth and astaxanthin accumulation in the unicellular green algaHaematococcus pluvialisas revealed by a proteomics approach[J]. Journal of Oceanology and Limnology, 2022, 40(5): 2052-2067.
Sodium acetate can promote the growth and astaxanthin accumulation in the unicellular green algaHaematococcus pluvialisas revealed by a proteomics approach[J]. 海洋湖沼学报（英文）, 2022,40(5):2052-2067. DOI： 10.1007/s00343-021-1271-y.

Xinlei YU, Xin YE, Chaoyang HU, et al. Sodium acetate can promote the growth and astaxanthin accumulation in the unicellular green algaHaematococcus pluvialisas revealed by a proteomics approach[J]. Journal of Oceanology and Limnology, 2022, 40(5): 2052-2067. DOI： 10.1007/s00343-021-1271-y.

摘要

Abstract

Haematococcus pluvialis

is an ideal natural source of strong antioxidant astaxanthin. Sodium acetate (NaAc) was proven an effective organic carbon source for improving algal growth and astaxanthin production; however

the underlying mechanism remains obscure. To reveal the mechanism of NaAc at the green vegetative stage of

pluvialis

the physiochemical characteristics and the global protein expression profiles obtained using a tandem mass tag labeling approach were compared between the control (CK) and two NaAc-addition groups. Results show that after NaAc addition

the biomass

nitrate consumption rate

and activities of three carbohydrate metabolism enzymes of

pluvialis

were significantly increased

and the net photosynthetic rate and chlorophyll content decreased. In addition

astaxanthin

total carbohydrates

and total lipids were accumulated

and some red cells appeared in the NaAc5 group. Moreover

317 differentially expressed proteins (DEPs) with the most altered expression patterns were screened out in the CK vs. NaAc5 comparison in our proteomics study. All the DEPs involved in carbohydrate metabolism and lipid metabolism were significantly increased

while most of the photosynthesis-related proteins were depressed in the two NaAc-treated groups. The proteomics results were verified and supported by parallel reaction monitoring approach and physiochemical data. Our findings demonstrate that NaAc promoted the tricarboxylic acid cycle

glyoxylate cycle

and amino acid and lipid synthesis

and inhibited the photosynthesis-related activities

which consequently speeded up the growth and astaxanthin accumulation in this alga.

关键词

Keywords

references

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Sodium acetate can promote the growth and astaxanthin accumulation in the unicellular green algaHaematococcus pluvialisas revealed by a proteomics approach

Sodium acetate can promote the growth and astaxanthin accumulation in the unicellular green algaHaematococcus pluvialisas revealed by a proteomics approach

DOI：10.1007/s00343-021-1271-y

摘要

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