Metabolomic and proteomic responses of<italic style="font-style: italic">Phaeodactylum tricornutum</italic>to hypoxia

Peipei ZHAO; Qinghua WU; Xuekui XIA; Shiyi GUO; Sizhong SHEN; Yujue WANG; Aiyou HUANG

doi:10.1007/s00343-021-1232-5

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Metabolomic and proteomic responses ofPhaeodactylum tricornutumto hypoxia

Ecology | Updated：2023-05-19

- Metabolomic and proteomic responses ofPhaeodactylum tricornutumto hypoxia
- Metabolomic and proteomic responses ofPhaeodactylum tricornutumto hypoxia
- 海洋湖沼学报（英文） 2022年40卷第5期页码：1963-1973
- Affiliations：
  
  1.Key Biosensor Laboratory of Shandong Province, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China
  2.State Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, China
  3.Laboratory of Development and Utilization of Marine Microbial Resource, Hainan University, Haikou 570228, China
  4.Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Haikou 570228, China
  5.College of Marine Sciences, Hainan University, Haikou 570228, China
  6.Department of Chemistry, University of Washington, Seattle WA 98195, USA
- Author bio：
  
  Aiyou HUANG,huangaiyou08@163.com
- Funds：
  
  the National Natural Science Foundation of China(41876158);the National Natural Science Foundation of China(31770024);the Biology and Biochemistry ESI Cultivation Discipline Open Project of Qilu University of Technology(ESIBBC202004);the Innovation and Development Joint Fund of Natural Science Foundation from Shandong Province(ZR2021LSW022);the Young Taishan Scholarship to Xuekui XIA(tsqn202103100);the Start-up Fund Project of Hainan University(KYQD(ZR)20060);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022Electronic supplementary materialSupplementary material (Supplementary Fig.S1) is available in the online version of this article athttps://doi.org/10.1007/s00343-021-1232-5.
- DOI：10.1007/s00343-021-1232-5
  中图分类号：
- 收稿：2021-07-16，
  
  录用：2021-9-1，
  
  网络首发：2021-11-04，
  
  纸质出版：2022-09
- Accepted：
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Metabolomic and proteomic responses ofPhaeodactylum tricornutumto hypoxia[J]. 海洋湖沼学报（英文）, 2022,40(5):1963-1973.

Peipei ZHAO, Qinghua WU, Xuekui XIA, et al. Metabolomic and proteomic responses ofPhaeodactylum tricornutumto hypoxia[J]. Journal of Oceanology and Limnology, 2022, 40(5): 1963-1973.
Metabolomic and proteomic responses ofPhaeodactylum tricornutumto hypoxia[J]. 海洋湖沼学报（英文）, 2022,40(5):1963-1973. DOI： 10.1007/s00343-021-1232-5.

Peipei ZHAO, Qinghua WU, Xuekui XIA, et al. Metabolomic and proteomic responses ofPhaeodactylum tricornutumto hypoxia[J]. Journal of Oceanology and Limnology, 2022, 40(5): 1963-1973. DOI： 10.1007/s00343-021-1232-5.

摘要

Abstract

Diatoms are important contributors to global net primary productivity

and play a crucial role in the biogeochemical cycles of carbon

phosphorus

nitrogen

iron

and silicon. Currently in some regions in the ocean

there's a trend that carbon content is high while oxygen concentration is low

and the underlying mechanisms of diatoms' response to low oxygen environments are worth investigating.

Phaeodactylum tricornutum

is a model diatom whose genome has been sequenced; it provides a universal molecular toolbox and a stable transgenic expression system. Therefore

the study of the responses of

tricornutum

to low oxygen has not only fundamental research significance but also important ecological significance. In this study

growth rates were determined and proteomic analysis and metabolomic analysis were performed to examine

tricornutum

responses under different oxygen concentrations (2% oxygen concentration for hypoxic condition and 21% oxygen concentration for the normal condition (sterilized air)). Results show that the hypoxic environment inhibited the growth of

tricornutum

. In the hypoxic conditions

tricornutum

could reset its metabolism pathways

including enhancement in lipid utilization

replenishment of tricarboxylic acid (TCA) cycle through the glyoxylic acid cycle

and down-regulation of photorespiration to reduce energy waste. Additionally

the stress resistance mechanism was activated to facilitate the adaptation to low oxygen conditions. This study helps to reveal the different metabolic changes to hypoxia of diatom from that of higher plants

which might be ascribed to their different habitats and needs further exploration in the future.

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references

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Metabolomic and proteomic responses ofPhaeodactylum tricornutumto hypoxia

Metabolomic and proteomic responses ofPhaeodactylum tricornutumto hypoxia

DOI：10.1007/s00343-021-1232-5

摘要

Abstract

关键词

Keywords

references