Responses of phytoplankton and its satellite bacteria to exogenous ethanol

Rediat ABATE; Buce Hanoch HETHARUA; Vishal PATIL; Daner LIN; Demeke KIFLE; Junrong LIANG; Changping CHEN; Lin SUN; Shuh-Ji KAO; Yonghong BI; Bangqin HUANG; Yahui GAO

doi:10.1007/s00343-021-1224-5

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Responses of phytoplankton and its satellite bacteria to exogenous ethanol

Ecology | Updated：2023-04-27

- Responses of phytoplankton and its satellite bacteria to exogenous ethanol
- Responses of phytoplankton and its satellite bacteria to exogenous ethanol
- 海洋湖沼学报（英文） 2023年41卷第1期页码：203-214
- Affiliations：
  
  1.Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  2.School of Life Sciences, Xiamen University, Xiamen 361102, China
  3.Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, College of Environment & Ecology, Xiamen University, Xiamen 361102, China
  4.State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen 361101, China
  5.Department of Zoological Sciences, Addis Ababa University, Addis Ababa, PO Box 1176, Ethiopia
- Author bio：
  
  Bangqin HUANG, E-mail: bqhuang@xmu.edu.cn
  Yahui GAO, E-mail: gaoyh@xmu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(42076114);the National Natural Science Foundation of China(41876146);the China Postdoctoral Science Foundation Grant(2018M632580);the Special Investigation Project of Scientific and Technological Fundamental Resources(2018FY100202);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2023Electronic supplementary material Supplementary material (Supplementary Figs.S1–S5) is available in the online version of this article at https://doi.org/10.1007/s00343-021-1224-5.
- DOI：10.1007/s00343-021-1224-5
  中图分类号：
- 收稿：2021-07-13，
  
  录用：2021-11-4，
  
  网络首发：2021-12-29，
  
  纸质出版：2023-01
- Accepted：
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Responses of phytoplankton and its satellite bacteria to exogenous ethanol[J]. 海洋湖沼学报（英文）, 2023,41(1):203-214.

Rediat ABATE, Buce Hanoch HETHARUA, Vishal PATIL, et al. Responses of phytoplankton and its satellite bacteria to exogenous ethanol[J]. Journal of Oceanology and Limnology, 2023, 41(1): 203-214.
Responses of phytoplankton and its satellite bacteria to exogenous ethanol[J]. 海洋湖沼学报（英文）, 2023,41(1):203-214. DOI： 10.1007/s00343-021-1224-5.

Rediat ABATE, Buce Hanoch HETHARUA, Vishal PATIL, et al. Responses of phytoplankton and its satellite bacteria to exogenous ethanol[J]. Journal of Oceanology and Limnology, 2023, 41(1): 203-214. DOI： 10.1007/s00343-021-1224-5.

摘要

Abstract

The response of phytoplankton and its satellite bacteria to various concentrations (0.01%Ƀ10% v/v) of ethanol is studied. To elucidate the effect of ethanol

single-strains of phytoplankton (SSP) culture

pure strains of satellite bacteria isolated from nonaxenic SSP cultures

and Escherichia coli were screened. Results indicate that ethanol could promote the growth and photosynthetic efficiency (

) of SSP at 0.01% and the growth of satellite bacteria at 0.01%Ƀ1%. Nevertheless

ethanol inhibited the growth and

of SSP at 0.1%Ƀ1%

and killed bacteria and SSP at 10% concentration. Further investigation on a satellite bacterium (

Mameliella alba

) revealed that ethanol promotes growth by serving as a growth stimulant rather than a metabolic carbon source. The 16S rRNA gene amplicon indicated that all nonaxenic SSP cultures harbor distinct satellite bacteria communities where the SSP culture of

Skeletonema costatum

Phaeodactylum tricornutum

and

Dunaliella bardawil

were dominated by bacteria genera of

Marivita

(~80%)

Dinoroseobacter

(~47%)

and

Halomonas

(~87%)

respectively

indicating that every SSP cultures have their own distinct satellite bacterial community. The bacteria family Rhodobacteraceae was dominant in the two marine diatoms

whereas Halomonadaceae was dominant in the saline green microalga. Compared to their respective controls

the supply of 0.5% ethanol to SSP cultures promoted the growth of the satellite bacteria but did not cause a significant difference in species composition of satellite bacteria. Therefore

a low concentration of ethanol can promote the growth of bacteria in a non-selective way. This study enriched our knowledge about the effect of ethanol on aquatic microbes and provided a baseline for basic and applied biotechnological research in the aquatic environment in the future.

关键词

Keywords

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