Temporal change of plankton size structure preserved by Lugol's solution: a FlowCAM study

Zijia LIU; Yuan DONG; Qian P. LI; Zhengchao WU; Zaiming GE; Mengzhen MA

doi:10.1007/s00343-021-1155-1

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Temporal change of plankton size structure preserved by Lugol's solution: a FlowCAM study

Biology | Updated：2023-04-27

- Temporal change of plankton size structure preserved by Lugol's solution: a FlowCAM study
- Temporal change of plankton size structure preserved by Lugol's solution: a FlowCAM study
- 海洋湖沼学报（英文） 2023年41卷第1期页码：290-299
- Affiliations：
  
  1.State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
  2.University of Chinese Academy of Sciences, Beijing 100049, China
  3.Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou), Guangzhou 511458, Chin
- Author bio：
  
  Yuan DONG, E-mail: dongyuan@scsio.ac.cn
  Qian P. LI, E-mail: qianli@scsio.ac.cn
- Funds：
  
  the Guangdong Province Special Support Plan for Leading Talents(2019TX05H216);the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)(GML2019ZD0305);the National Natural Science Foundation of China(41906132);the Science and Technology Program of Guangzhou(202102021229);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2023Electronic supplementary material Supplementary material (Supplementary Fig.S1) is available in the online version of this article at https://doi.org/10.1007/s00343-021-1155-1.
- DOI：10.1007/s00343-021-1155-1
  中图分类号：
- 收稿：2021-05-20，
  
  录用：2021-9-23，
  
  网络首发：2021-12-29，
  
  纸质出版：2023-01
- Accepted：
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Temporal change of plankton size structure preserved by Lugol's solution: a FlowCAM study[J]. 海洋湖沼学报（英文）, 2023,41(1):290-299.

Zijia LIU, Yuan DONG, Qian P. LI, et al. Temporal change of plankton size structure preserved by Lugol's solution: a FlowCAM study[J]. Journal of Oceanology and Limnology, 2023, 41(1): 290-299.
Temporal change of plankton size structure preserved by Lugol's solution: a FlowCAM study[J]. 海洋湖沼学报（英文）, 2023,41(1):290-299. DOI： 10.1007/s00343-021-1155-1.

Zijia LIU, Yuan DONG, Qian P. LI, et al. Temporal change of plankton size structure preserved by Lugol's solution: a FlowCAM study[J]. Journal of Oceanology and Limnology, 2023, 41(1): 290-299. DOI： 10.1007/s00343-021-1155-1.

摘要

Abstract

Plankton size structure is crucial for understanding marine ecosystem dynamics and the associated biogeochemical processes. A fixation step by acid Lugol's solution has been commonly employed to preserve plankton samples in the field. However

the acid Lugol's solution can bias the estimation of size structure and the preserved plankton size structure can vary with time. Here

we explore the impact of sample storage time on the size-structure of the plankton community preserved by Lugol's solution. Two short-term experiments and one long-term experiment were conducted to explore the change of plankton community size structure with the storage time: covering from a week to a month

and to nearly seven months based on particle-size data obtained by continuous Flow Cytometer and Microscope (FlowCAM) measurements. We found a linear change of plankton size with the storage time in short-term periods (less than 3 months) with a decrease of the slope but an increase of the intercept for the normalized biomass size spectrum (NBSS). However

there were opposite trends for NBSS with increasing slope but decreasing intercept after 3 months. The potential causes of the distinct patterns of the NBSS parameters are addressed in terms of the interplay between particle aggregation and fragmentation. We found large changes in plankton biovolume and abundance among different size classes

which may indicate a distinct effect of acid Lugol's solution on various plankton size classes. The mechanism driving temporal change in the size-structure of the Lugolfixed plankton community was further discussed in terms of particle aggregation and fragmentation. Finally

we emphasize that the effect of storage time should be taken into account when interpreting or comparing data of plankton community acquired from samples with various storage durations.

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references

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