Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of <italic style="font-style: italic">Pyropia yezoensis</italic>

Yunke ZHU; Xinran WANG; Bin SUN; Xianghai TANG; Yunxiang MAO

doi:10.1007/s00343-021-1141-7

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Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis

Biology | Updated：2023-05-20

- Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis
- Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis
- 海洋湖沼学报（英文） 2022年40卷第3期页码：1148-1159
- Affiliations：
  
  1.Key Laboratory of Marine Genetics and Breeding (Ministry of Education), College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
  2.MOE Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources, College of Fisheries and Life Science, Hainan Tropical Ocean University, Sanya 572022, China
  3.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
- Author bio：
  
  Xianghai TANG, txianghai@ouc.edu.cn
  Yunxiang MAO, yxmao@hntou.edu.cn
- Funds：
  
  the National Key R & D Program of China(2018YFD0900106);the National Key R&D Program of China(2020YFD0901101);the Marine S & T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)(2018SDKJ0302-4);the Fundamental Research Funds for the Central Universities(202064006);the MOA Modern Agricultural Talents Support Project;Special Project of Central Government Guiding Local Science and Technology Development(7033204020);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-021-1141-7
  中图分类号：
- 纸质出版：2022-03
- Accepted：
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Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis[J]. 海洋湖沼学报（英文）, 2022,40(3):1148-1159.

Yunke ZHU, Xinran WANG, Bin SUN, et al. Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1148-1159.
Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis[J]. 海洋湖沼学报（英文）, 2022,40(3):1148-1159. DOI： 10.1007/s00343-021-1141-7.

Yunke ZHU, Xinran WANG, Bin SUN, et al. Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1148-1159. DOI： 10.1007/s00343-021-1141-7.

摘要

Abstract

The phosphatidylinositol (PI) signaling system

a central regulator of eukaryotic metabolism

is widely found in eukaryotes for regulating a variety of cell activities. Most of the genes in the PI signaling system were found conserved in

Pyropia yezoensis

. In this experiment

wortmannin was used as an inhibitor to inhibit the activity of phosphatidylinositol-3 kinase (

PI3K

)

an important regulator of the PI signaling system. After wortmannin treatment

the mitotic division of

P. yezoensis

was significantly inhibited in a dose-dependent manner

and the mitotic division percentage was reduced by 68.1% and 91.9% in the 5-and 10-µmol/L groups

respectively. When thalli were treated with wortmannin

the levels of reactive oxygen species (ROS) were significantly decreased. Furthermore

the expression level of

PI3K

was inhibited and the expression levels of downstream genes regulated by

PI3K

was significantly changed. In the

PI3K-AGC

signaling pathway

the expression levels of Serine/threonine protein kinase (

AGC

) and cyclin-dependent kinases A (

CDKA

) were downregulated

while WEE1 kinase gene (

WEE1

) was upregulated. Three nicotinamide adenine dinucleotide phosphate (NADPH) oxidase genes were downregulated after wortmannin treatment. These results indicate that the PI signaling system plays an important role in the regulation of cell activity in

P. yezoensis

. It was speculated that the growth and development of

P. yezoensis

might be regulated by

P. yezoensis PI3K

which promoted the expression of the

AGC

gene and further regulates the expression of downstream

WEE1

and

CDKA

genes to advance mitotic division

and also promoted the expression level of NADPH oxidase that regulates ROS homeostasis.

关键词

Keywords

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Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis

Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis

DOI：10.1007/s00343-021-1141-7

摘要

Abstract

关键词

Keywords

references