Immunostimulatory effect of quaternary degree and acetyl group of quaternized chitosan on macrophages RAW 264.7

Xiaomin LI; Rong'e XING; Chaojie XU; Song LIU; Yukun QIN; Kecheng LI; Huahua YU; Pengcheng LI

doi:10.1007/s00343-021-1098-6

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Immunostimulatory effect of quaternary degree and acetyl group of quaternized chitosan on macrophages RAW 264.7

Biology | Updated：2023-05-20

- Immunostimulatory effect of quaternary degree and acetyl group of quaternized chitosan on macrophages RAW 264.7
- Immunostimulatory effect of quaternary degree and acetyl group of quaternized chitosan on macrophages RAW 264.7
- 海洋湖沼学报（英文） 2022年40卷第3期页码：1160-1170
- Affiliations：
  
  1.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266237, China
  2.Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  Rong'e XING, xingronge@qdio.ac.cn
- Funds：
  
  the Key Deployment Projects of the Marine Science Research Center of the Chinese Academy of Sciences(COMS2020J04);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-021-1098-6
  中图分类号：
- 纸质出版：2022-03
- Accepted：
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Immunostimulatory effect of quaternary degree and acetyl group of quaternized chitosan on macrophages RAW 264.7[J]. 海洋湖沼学报（英文）, 2022,40(3):1160-1170.

Xiaomin LI, Rong'e XING, Chaojie XU, et al. Immunostimulatory effect of quaternary degree and acetyl group of quaternized chitosan on macrophages RAW 264.7[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1160-1170.
Immunostimulatory effect of quaternary degree and acetyl group of quaternized chitosan on macrophages RAW 264.7[J]. 海洋湖沼学报（英文）, 2022,40(3):1160-1170. DOI： 10.1007/s00343-021-1098-6.

Xiaomin LI, Rong'e XING, Chaojie XU, et al. Immunostimulatory effect of quaternary degree and acetyl group of quaternized chitosan on macrophages RAW 264.7[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1160-1170. DOI： 10.1007/s00343-021-1098-6.

摘要

Abstract

Hydroxypropyltrimethyl ammonium chloride chitosan (HACC) and hydroxypropyltrimethyl ammonium chloride fully deacetylated chitosan (De-HACC) were synthesized with various degrees of substitution by altering the ratio of chitosan to glycidyl trimethyl-ammonium chloride (GTMAC). The effects of the quaternary ammonium degree and the acetyl group of these polymers on immunostimulatory activities were detected in RAW 264.7 cells. The expression levels of nitrogen oxide (NO)

interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) were compared. Results show that the removal of acetyl groups in chitosan obviously improved the degree of substitution of quaternary ammonium salts. In addition

HACC and De-HACC were capable of promoting immunological activity in a substitution-dependent manner; HACC was positively correlated

and De-HACC was negatively correlated. Among tested ratios

HACC-30% and De-HACC-54% performed better than the others

and De-HACC-54% performed the best. Generally

quaternized chitosan possesses immunostimulatory activity

which is related to the degree of quaternization and the acetyl group.

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references

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