Microbial community coexisting with harmful alga <italic style="font-style: italic">Karenia mikimotoi</italic> and microbial control of algal bloom in laboratory

Li SUN; Peike GAO; Yu LI; Chao WANG; Ning DING; Junfeng CHEN; Yuhao SONG; Chunchen LIU; Lun SONG; Renjun WANG

doi:10.1007/s00343-021-1087-9

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Microbial community coexisting with harmful alga Karenia mikimotoi and microbial control of algal bloom in laboratory

Ecology | Updated：2023-05-20

- Microbial community coexisting with harmful alga Karenia mikimotoi and microbial control of algal bloom in laboratory
- Microbial community coexisting with harmful alga Karenia mikimotoi and microbial control of algal bloom in laboratory
- 海洋湖沼学报（英文） 2022年40卷第3期页码：1027-1038
- Affiliations：
  
  1.College of Life Sciences, Qufu Normal University, Qufu, 273165, China
  2.Key Laboratory of Marine Biological Resources and Ecology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, 116023, China
- Author bio：
  
  Peike GAO, gpkyll-001@163.com
  Renjun WANG, wangrenjun2002@126.com
- Funds：
  
  the National Natural Science Foundation of China(31971503);the National Natural Science Foundation of China(31901188);the Agricultural Variety Improvement Project of Shandong Province(2019LZGC020);the Jining Key Research and Development Project of Shandong Province(2019ZDGH019);the Shandong Provincial Natural Science Foundation(ZR2019BB040);the Shandong Provincial Natural Science Foundation(ZR2020MC042);the Interdisciplinary Project of Qufu Normal University(XKJJC201903);the Key Research and Development Project of Liaoning Province(2018228004);the Revitalization Talents Program of Liaoning Province(XLYC1907109);the Shandong Provincial Key Research and Development Project(2018GSF117035);the Shandong Provincial Higher Educational Science and Technology Program(J17KA112);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-021-1087-9
  中图分类号：
- 纸质出版：2022-03
- Accepted：
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Microbial community coexisting with harmful alga Karenia mikimotoi and microbial control of algal bloom in laboratory[J]. 海洋湖沼学报（英文）, 2022,40(3):1027-1038.

Li SUN, Peike GAO, Yu LI, et al. Microbial community coexisting with harmful alga Karenia mikimotoi and microbial control of algal bloom in laboratory[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1027-1038.
Microbial community coexisting with harmful alga Karenia mikimotoi and microbial control of algal bloom in laboratory[J]. 海洋湖沼学报（英文）, 2022,40(3):1027-1038. DOI： 10.1007/s00343-021-1087-9.

Li SUN, Peike GAO, Yu LI, et al. Microbial community coexisting with harmful alga Karenia mikimotoi and microbial control of algal bloom in laboratory[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1027-1038. DOI： 10.1007/s00343-021-1087-9.

摘要

Abstract

Algicidal bacteria have been frequently isolated from algal blooming areas. However

knowledge regarding the microbial communities coexisting with microalgae and their potential application in preventing harmful algal blooms (HABs) is limited. In this study

we investigated the composition of the microbial community coexisting with harmful alga

Karenia mikimotoi

and its responses to algal control via nutrient stimulation or by adding algicidal strain in microcosms. The microorganisms inhabiting the

K. mikimotoi

culture consisted of 24 identified phyla

including dominant Proteobacteria (relative abundance 76.24%±7.28%) and Bacteroidetes (22.67%±8.32%). Rhodobacteraceae

Phaeodactylibacter

and

Maritimibacter

predominated during the algal cultivation. Both the added nutrient and fermentation broth of algicidal strain

Pseudoalteromonas

QF1 caused a massive death of

K. mikimotoi

and substantial changes in the coexisting microbial community

in which Rhodobacteraceae and

Phaeodactylibacter

significantly decreased

while

Halomonas

and

Alteromonas

increased. Core operational taxonomic units (OTUs) analysis indicated that 13 OTUs belonging to Rhodobacteraceae

Maritimibacter

Marivita

Nisaea

Phaeodactylibacter

Citreicella

Halomonas

Alteromonas

Marinobacter

Muricauda

and

Pseudoalteromonas

dominated the changes of the microbial communities observed in the

K. mikimotoi

culture with or without treatments. Collectively

this study indicated that microbial community inhabiting

K. mikimotoi

culture includes potential algicidal bacteria

and improves our knowledge about microbial community succession during biocontrol of

K. mikimotoi

via nutrient stimulation or by adding isolated algicidal strains.

关键词

Keywords

references

Amin S A, Parker M S, Armbrust E V. 2012. Interactions between diatoms and bacteria. Microbiology and Molecular Biology Reviews , 76 (3): 667–684, https://doi.org/10.1128/MMBR.00007-12..

Aoki K, Kameda T, Yamatogi T, Ishida N, Hirae S, Kawaguchi M, Syutou T. 2017. Spatio-temporal variations in bloom of the red-tide dinoflagellate Karenia mikimotoi in Imari Bay, Japan, in 2014: factors controlling horizontal and vertical distribution. Marine Pollution Bulletin , 124 (1): 130–138, https://doi.org/10.1016/j.marpolbul.2017.07.019..

Baker L J, Kemp P F. 2014. Exploring bacteria-diatom associations using single-cell whole genome amplification. Aquatic Microbial Ecology , 72 (1): 73–88, https://doi.org/10.3354/ame01686..

Burke C, Thomas T, Lewis M, Steinberg P, Kjelleberg S. 2011. Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis . The ISME Journal , 5 (4): 590–600, https://doi.org/10.1038/ismej.2010.164..

Burnham J C, Collart S A, Daft M J. 1984. Myxococcal predation of the cyanobacterium Phormidium luridum in aqueous environments. Archives of Microbiology , 137 (3): 220–225, https://doi.org/10.1007/BF00414547..

Cai J, Chen D Y, Chen D, Huang X H, Li C L, Liu H L, Li M J, Li G B, Zhang Y L. 2019. Complete genome sequence of Brevibacillus laterosporus Bl-zj, an algicidal bacterium isolated from soil. Microbiology Resource Announcements , 8 (30): e00408–19, https://doi.org/10.1128/MRA.00408-19..

Caporaso J G, Kuczynski J, Stombaugh J, Bittinger K, Bushman F D, Costello E K, Fierer N, Peña A G, Goodrich J K, Gordon J I, Huttley G A, Kelley S T, Knights D, Koenig J E, Ley R E, Lozupone C A, Mcdonald D, Muegge B D, Pirrung M, Reeder J, Sevinsky J R, Turnbaugh P J, Walters W A, Widmann J, Yatsunenko T, Zaneveld J, Knight R. 2010. QIIME allows analysis of high-throughput community sequencing data. Nature Methods , 7 (5): 335–336, https://doi.org/10.1038/nmeth.f.303..

Chen Z R, Lei X Q, Lai Q L, Li Y, Zhang B Z, Zhang J Y, Zhang H J, Yang L X, Zheng W, Tian Y, Yu Z M, Xu H, Zheng T L. 2014. Phaeodactylibacter xiamenensis gen. nov., sp. nov., a member of the family Saprospiraceae isolated from the marine alga Phaeodactylum tricornutum . International Journal of Systematic and Evolutionary Microbiology , 64 (Pt10): 3496–3502, https://doi.org/10.1099/ijs.0.063909-0..

Closson K R, Paul E A. 2014. Comparison of the toxicity of two chelated copper algaecides and copper sulfate to non-target fish. Bulletin of Environmental Contamination and Toxicology , 93 (6): 660–665, https://doi.org/10.1007/s00128-014-1394-3..

Cole J R, Wang Q, Fish J A, Chai B L, McGarrell D M, Sun Y N, Brown C T, Porras-Alfaro A, Kuske C R, Tiedje J M. 2014. Ribosomal Database Project: data and tools for high throughput rRNA analysis. Nucleic Acids Research , 42 (D1): D633–D642, https://doi.org/10.1093/nar/gkt1244..

Edgar R C. 2013. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods , 10 (10): 996–998, https://doi.org/10.1038/nmeth.2604..

Gillibrand P A, Siemering B, Miller P I, Davidson K. 2016. Individual-based modelling of the development and transport of a Karenia mikimotoi bloom on the North-west European continental shelf. Harmful Algae , 53 : 118–134, https://doi.org/10.1016/j.hal.2015.11.011..

Guillard R R L, Ryther J H. 1962. Studies of marine planktonic diatoms: I. Cyclotella nana Hustedt, and Detonula confervacea (Cleve) Gran. Canadian Journal of Microbiology , 8 (2): 229–239, https://doi.org/10.1139/m62-029..

Imai I, Fujimaru D, Nishigaki T, Kurosaki M, Sugita H. 2006. Algicidal bacteria isolated from the surface of seaweeds from the coast of Osaka Bay in the Seto Inland Sea, Japan. African Journal of Marine Science , 28 (2): 319–323, https://doi.org/10.2989/18142320609504170..

Kok J W K, Leong S C Y. 2019. Nutrient conditions and the occurrence of a Karenia mikimotoi (Kareniaceae) bloom within East Johor Straits, Singapore. Regional Studies in Marine Science , 27 : 100514, https://doi.org/10.1016/j.rsma.2019.100514..

Kumar P S, Kumaraswami M, Rao G D, Ezhilarasan P, Sivasankar R, Rao V R, Ramu K. 2018. Influence of nutrient fluxes on phytoplankton community and harmful algal blooms along the coastal waters of southeastern Arabian Sea. Continental Shelf Research , 161 : 20–28, https://doi.org/10.1016/j.csr.2018.04.012..

Lee B K, Katano T, Kitamura S I, Oh M J, Han M S. 2008. Monitoring of algicidal bacterium, Alteromonas sp. strain A14 in its application to natural Cochlodinium polykrikoides blooming seawater using fluorescence in situ hybridization. The Journal of Microbiology , 46 (3): 274–282, https://doi.org/10.1007/s12275-007-0238-9..

Lee S O, Kato J, Takiguchi N, Kuroda A, Ikeda T, Mitsutani A, Ohtake H. 2000. Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28. Applied and Environmental Microbiology , 66 (10): 4334–4339, https://doi.org/10.1128/AEM.66.10.4334-4339.2000..

Lei X Q, Li Y, Wang G H, Chen Y, Lai Q L, Chen Z R, Zhang J Y, Liao P P, Zhu H, Zheng W, Zheng T L. 2015. Phaeodactylibacter luteus sp. nov., isolated from the oleaginous microalga Picochlorum sp. International Journal of Systematic and Evolutionary Microbiology , 65 (8): 2666–2670, https://doi.org/10.1099/IJS.0.000321..

Li X D, Yan T, Lin J N, Yu R C, Zhou M J. 2017. Detrimental impacts of the dinoflagellate Karenia mikimotoi in Fujian coastal waters on typical marine organisms. Harmful Algae , 61 : 1–12, https://doi.org/10.1016/j.hal.2016.11.011..

Li Y Y, Yu J F, Sun T K, Liu C C, Sun Y, Wang Y. 2018. Using the marine rotifer Brachionus plicatilis as an endpoint to evaluate whether ROS-Dependent hemolytic toxicity is involved in the allelopathy induced by Karenia mikimotoi . Toxins , 10 (11): 439, https://doi.org/10.3390/toxins10110439..

Liu J, Li F C, Liu L, Jiang P, Liu Z P. 2013. Inhibitory activity of an extract from a marine bacterium Halomonas sp. HSB07 against the red-tide microalga Gymnodinium sp. (Pyrrophyta). Chinese Journal of Oceanology and Limnology , 31 (6): 1241–1247, https://doi.org/10.1007/s00343-013-3160-5..

Lovejoy C, Bowman J P. 1998. Algicidal effects of a novel Marine pseudoalteromonas isolate (class proteobacteria, gamma subdivision) on harmful algal bloom species of the genera Chattonella, gymnodinium , and Heterosigma . Applied and Environmental Microbiology , 64 (8): 2806–2813, https://doi.org/10.1128/AEM.64.8.2806-2813.1998..

Lu X H, Zhou B, Xu L L, Liu L, Wang G Y, Liu X D, Tang X X. 2016. A marine algicidal Thalassospira and its active substance against the harmful algal bloom species Karenia mikimotoi . Applied Microbiology and Biotechnology , 100 (11): 5131–5139, https://doi.org/10.1007/s00253-016-7352-8..

Lyu Y H, Zhou Y X, Li Y, Zhou J, Xu Y X. 2019. Optimized culturing conditions for an algicidal bacterium Pseudoalteromonas sp. SP48 on harmful algal blooms caused by Alexandrium tamarense . MicrobiologyOpen , 8 (8): e00803, https://doi.org/10.1002/mbo3.803..

Mcilroy S J, Nielsen P H. 2014. The family Saprospiraceae. In: Rosenberg E, DeLong E F, Lory S, Stackebrandt E, Thompson F eds. The Prokaryotes: Other Major Lineages of Bacteria and the Archaea. Springer, Berlin. p. 863–889, https://doi.org/10.1007/978-3-642-38954-2_138 https://doi.org/10.1007/978-3-642-38954-2_138 ..

Mizuno K I, Wakano M, Takatsuji H, Nagai T. 2015. Effects of the dinoflagellate Karenia mikimotoi on larval settlement of Pacific oyster Crassostrea gigas . Nippon Suisan Gakkaishi , 81 (5): 811–816, https://doi.org/10.2331/suisan.81.811..

Nagai T, Taya K, Yoda I. 2016. Comparative toxicity of 20 herbicides to 5 periphytic algae and the relationship with mode of action. Environmental Toxicology and Chemistry , 35 (2): 368–375, https://doi.org/10.1002/etc.3150..

O'Boyle S, Mcdermott G, Silke J, Cusack C. 2016. Potential impact of an exceptional bloom of Karenia mikimotoi on dissolved oxygen levels in waters off western Ireland. Harmful Algae , 53 : 77–85, https://doi.org/10.1016/j.hal.2015.11.014..

Oh J I, Kim M J, Lee J Y, Ko I J, Kim W, Si W K. 2011. Isolation and characterization of algicidal bacteria from Cochlodinium polykrikoides culture. Biotechnology and Bioprocess Engineering , 16 (6): 1124–1133, https://doi.org/10.1007/s12257-011-0232-2..

Park J, Church J, Son Y, Kim K T, Lee W H. 2017. Recent advances in ultrasonic treatment: challenges and field applications for controlling harmful algal blooms (HABs). Ultrasonics Sonochemistry , 38 : 326–334, https://doi.org/10.1016/j.ultsonch.2017.03.003..

Pujalte M J, Lucena T, Ruvira M A, Arahal D R, Macián M C. 2014. The family Rhodobacteraceae . In: Rosenberg E, DeLong E F, Lory S, Stackebrandt E, Thompson F eds. The Prokaryotes: Alphaproteobacteria and Betaproteobacteria. Springer, Berlin, Heidelberg, p. 439–512, https://doi.org/10.1007/978-3-642-30197-1_377 https://doi.org/10.1007/978-3-642-30197-1_377 ..

Sakamoto S, Lim W A, Lu D D, Dai X F, Orlova T, Iwataki M. 2021. Harmful algal blooms and associated fisheries damage in East Asia: current status and trends in China, Japan, Korea and Russia. Harmful Algae , 102 : 101787, https://doi.org/10.1016/j.hal.2020.101787..

Shao Q W, Lin Z Z, Zhou C X, Zhu P, Yan X J. 2020. Succession of bacterioplankton communities over complete Gymnodinium -diatom bloom cycles. Science of the Total Environment , 709 : 135951, https://doi.org/10.1016/j.scitotenv.2019.135951..

Shimizu T, Oda T, Ito H, Imai I. 2017. Isolation and characterization of algicidal bacteria and its effect on a musty odor-producing cyanobacterium Dolichospermum crassum in a reservoir. Water Supply , 17 (3): 792–798, https://doi.org/10.2166/ws.2016.179..

Sourisseau M, Jegou K, Lunven M, Quere J, Gohin F, Bryere P. 2016. Distribution and dynamics of two species of Dinophyceae producing high biomass blooms over the French Atlantic Shelf. Harmful Algae , 53 : 53–63, https://doi.org/10.1016/j.hal.2015.11.016..

Su J F, Shao S C, Huang T L, Ma F, Zhang K, Wen G, Zheng S C. 2016. Isolation, identification, and algicidal activity of aerobic denitrifying bacterium R11 and its effect on Microcystis aeruginosa . Water Science & Technology , 73 (11): 2600–2607, https://doi.org/10.2166/wst.2016.085..

Sun H Y, Zhang Y, Chen H R, Hu C X, Li H, Hu Z L. 2016. Isolation and characterization of the marine algicidal bacterium Pseudoalteromonas S1 against the harmful alga Akashiwo sanguinea . Marine Biology , 163 (3): 66, https://doi.org/10.1007/s00227-016-2836-8..

Vandersea M, Tester P, Holderied K, Hondolero D, Kibler S, Powell K, Baird S, Doroff A, Dugan D, Meredith A, Tomlinson M, Litaker R W. 2020. An extraordinary Karenia mikimotoi "beer tide" in Kachemak Bay Alaska. Harmful Algae , 92 : 101706, https://doi.org/10.1016/j.hal.2019.101706..

Yu X Q, Cai G J, Wang H, Hu Z, Zheng W, Lei X Q, Zhu X Y, Chen Y, Chen Q L, Din H Y, Xu H, Tian Y, Fu L J, Zheng T L. 2018. Fast-growing algicidal Streptomyces sp. U3 and its potential in harmful algal bloom controls. Journal of Hazardous Materials , 341 : 138–149, https://doi.org/10.1016/j.jhazmat.2017.06.046..

Zhang S F, Han B B, Wu F X, Huang H H. 2020a. Quantitative proteomic analysis provides insights into the algicidal mechanism of Halobacillus sp. P1 against the marine diatom Skeletonema costatum . Science of the Total Environment , 717 : 137048, https://doi.org/10.1016/j.scitotenv.2020.137048..

Zhang S, Zheng W, Wang H. 2020b. Physiological response and morphological changes of Heterosigma akashiwo to an algicidal compound prodigiosin. Journal of Hazardous Materials , 385 : 121530, https://doi.org/10.1016/j.jhazmat.2019.121530..

Zheng N N, Ding N, Gao P K, Han M, Liu X X, Wang J G, Sun L, Fu B Y, Wang R J, Zhou J. 2018. Diverse algicidal bacteria associated with harmful bloom-forming Karenia mikimotoi in estuarine soil and seawater. Science of the Total Environment , 631-632 : 1415–1420, https://doi.org/10.1016/j.scitotenv.2018.03.035..

Zheng N N, Sun L, Ding N, Li C, Fu B Y, Wang C, Gao P K, Wang R J. 2019. Diversity of algicidal bacteria associated with harmful microalgae and the algicidal mechanisms. Microbiology China , 46 (5): 1204–1219, https://doi.org/10.13344/j.microbiol.china.180464. (in Chinese with English abstract).

Zheng N N. 2019. Algicidal bacteria associated with harmful bloom-forming Karenia mikimotoi in estuarine soil and study on the algicidal effect and mechanism. A Dissertation Submitted to Qufu Normal University. (in Chinese)

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Microbial community coexisting with harmful alga Karenia mikimotoi and microbial control of algal bloom in laboratory

DOI：10.1007/s00343-021-1087-9

摘要

Abstract

关键词

Keywords

references