Community structure and assembly of denitrifying bacteria in epiphytic biofilms in a freshwater lake ecosystem

Guoqing LI; Dingbo YAN; Pinhua XIA; Haipeng CAO; Tao LIN; Yin YI

doi:10.1007/s00343-021-1084-z

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Community structure and assembly of denitrifying bacteria in epiphytic biofilms in a freshwater lake ecosystem

Ecology | Updated：2023-05-20

- Community structure and assembly of denitrifying bacteria in epiphytic biofilms in a freshwater lake ecosystem
- Community structure and assembly of denitrifying bacteria in epiphytic biofilms in a freshwater lake ecosystem
- 海洋湖沼学报（英文） 2022年40卷第3期页码：1039-1050
- Affiliations：
  
  1.Guizhou Province Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, 550001, China
  2.College of Life Sciences, Guizhou Normal University, Guiyang, 550001, China
  3.State Key Laboratory of Southwest Karst Mountain Biodiversity Conservation of Forestry and Grassland Administration, Guizhou Normal University, Guiyang, 550001, China
- Author bio：
  
  Pinhua XIA, pinhuaxia@gznu.edu.cn
- Funds：
  
  the National Nature Science Foundation of China(41867056);the Karst Science Research Center of Guizhou Province(U1812401);the Guizhou Province Graduate Education Innovation Project(YJSCXJH(2019)048);the Guizhou Province Science and Technology Plan Project(2021470);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-021-1084-z
  中图分类号：
- 纸质出版：2022-03
- Accepted：
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Community structure and assembly of denitrifying bacteria in epiphytic biofilms in a freshwater lake ecosystem[J]. 海洋湖沼学报（英文）, 2022,40(3):1039-1050.

Guoqing LI, Dingbo YAN, Pinhua XIA, et al. Community structure and assembly of denitrifying bacteria in epiphytic biofilms in a freshwater lake ecosystem[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1039-1050.
Community structure and assembly of denitrifying bacteria in epiphytic biofilms in a freshwater lake ecosystem[J]. 海洋湖沼学报（英文）, 2022,40(3):1039-1050. DOI： 10.1007/s00343-021-1084-z.

Guoqing LI, Dingbo YAN, Pinhua XIA, et al. Community structure and assembly of denitrifying bacteria in epiphytic biofilms in a freshwater lake ecosystem[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1039-1050. DOI： 10.1007/s00343-021-1084-z.

摘要

Abstract

Denitrifying bacteria are a crucial component of aquatic ecosystem in nitrogen cycle. However

the denitrifying bacterial community dynamics and structure in epiphytic biofilms remain unexplored. The abundance of denitrification gene (

nir

) and structure of

nirS

-denitrifying bacterial community in the epiphytic biofilms collected in July and November of 2018 from a typical plateau lake (Caohai Wetland

Guizhou

China) were studied by Real-time Quantitative Polymerase Chain Reaction (qPCR) and high-throughput sequencing. Results show that the gene abundance of

nirK

was higher than that of

nirS

(

0.05)

and it was significantly different during the growth period (July) than the decline period (November). The denitrifying bacterial species was similar in the two months and shared 76.18% of OTUs. Proteobacteria (56.55%±22.15%) was the dominant phylum in all the samples. Epiphytic biofilms between growth period and decline period displayed significantly different microbial community structures due to differences in species abundance. Water temperature was the crucial factor that affected the denitrifying microbial community structure in our study. Environmental factors explain only partially the dynamic characteristics of denitrifying microbial communities

implying that the stochastic processes affected the construction of denitrifying microbial communities. As the null model analysis results show

dispersal limitation (stochastic) and undominated processes significantly influenced the assembly of denitrifying microbial communities. This study broadened our understanding of the denitrifying bacterial community structure and its function on epiphytic biofilms in freshwater ecosystems with new information provided.

关键词

Keywords

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