Carnosine concentration and expression profiles of carnosine related genes in <italic style="font-style: italic">Mytilus</italic> after beta-alanine injection

Chen PAN; Zhi LIAO; Jianyu HE; Zhongqi GU; Chunyue WANG; Meihua FAN; Isabella BUTTINO; Baoying GUO; Xiaojun YAN; Xiaolin ZHANG

doi:10.1007/s00343-021-1078-x

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Carnosine concentration and expression profiles of carnosine related genes in Mytilus after beta-alanine injection

Biology | Updated：2023-05-20

- Carnosine concentration and expression profiles of carnosine related genes in Mytilus after beta-alanine injection
- Carnosine concentration and expression profiles of carnosine related genes in Mytilus after beta-alanine injection
- 海洋湖沼学报（英文） 2022年40卷第3期页码：1121-1134
- Affiliations：
  
  1.Laboratory of Marine Biology Protein Engineering, Marine Science and Technical College, Zhejiang Ocean University, Zhoushan 316022, China
  2.Bureau of Science and Technology Shengsi, Zhoushan 316022, China
  3.Italian Institute for Environmental Protection and Research (ISPRA), Via Vitaliano Brancati 48, Rome 00144, Italy
- Author bio：
  
  Xiaojun YAN, yanxj@zjou.edu.cn
  Xiaolin ZHANG, zhangxiaolin@zjou.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(42020104009);the National Natural Science Foundation of China(31671009);the National Natural Science Foundation of China(42076119);the Project of Zhoushan Science and Technology Bureau(2019F12004);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-021-1078-x
  中图分类号：
- 纸质出版：2022-03
- Accepted：
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Carnosine concentration and expression profiles of carnosine related genes in Mytilus after beta-alanine injection[J]. 海洋湖沼学报（英文）, 2022,40(3):1121-1134.

Chen PAN, Zhi LIAO, Jianyu HE, et al. Carnosine concentration and expression profiles of carnosine related genes in Mytilus after beta-alanine injection[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1121-1134.
Carnosine concentration and expression profiles of carnosine related genes in Mytilus after beta-alanine injection[J]. 海洋湖沼学报（英文）, 2022,40(3):1121-1134. DOI： 10.1007/s00343-021-1078-x.

Chen PAN, Zhi LIAO, Jianyu HE, et al. Carnosine concentration and expression profiles of carnosine related genes in Mytilus after beta-alanine injection[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1121-1134. DOI： 10.1007/s00343-021-1078-x.

摘要

Abstract

Carnosine and its analogues are histidine-containing dipeptides (HCDs) playing diverse functions in vertebrates. However

the distribution and the metabolism of carnosine in invertebrates are still unknown. In this study

Mytilus coruscus

a shellfish with important economic value in China

was selected for the investigation of HCD content and the expression profiling of carnosine-related genes in various mussel tissues. Quantification of HCD by amino acids analyzer revealed a low concentration of anserine in muscular tissues in

Mytilus

indicating the presence of HCD even in an invertebrate. mRNA expression of five carnosine metabolic-related genes was profiled in various tissues

and the results highlighted the relative higher expression level of these genes in muscular tissues. Considering the fact that beta-alanine supplementation can increase the HCD content in vertebrates

a beta-alanine injection was performed and the changes of HCD concentration and the mRNA expression of carnosine related genes were investigated in five mussel tissues. The results revealed the increase of HCD concentration

as well as the up-regulated expression level of related genes

in tested tissues of beta-alanine injected mussels. Transcriptomic analysis for the whole soft tissue of mussel before and after beta-alanine injection were performed

and 3 569 differential expression genes (DEGs) were identified in the beta-alanine injected group when compared to their expression levels in the control. These data indicated the complex effects of beta-alanine on

M. coruscus

metabolism

and those DEGs enriched in pathways of cancers

muscle contraction

and tyrosine metabolism highlighted the possible functions of beta-alanine in cell proliferation

sports

and melanogenesis

respectively.

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Keywords

references

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Carnosine concentration and expression profiles of carnosine related genes in Mytilus after beta-alanine injection

Carnosine concentration and expression profiles of carnosine related genes in Mytilus after beta-alanine injection

DOI：10.1007/s00343-021-1078-x

摘要

Abstract

关键词

Keywords

references