Characterization of <italic style="font-style: italic">Amphidinium</italic> (Amphidiniales, Dinophyceae) species from the China Sea based on morphological, molecular, and pigment data

Zhaohe LUO; Hua ZHANG; Qun LI; Lei WANG; Hala F MOHAMED; Songhui LÜ; Haifeng GU

doi:10.1007/s00343-021-1049-2

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Characterization of Amphidinium (Amphidiniales, Dinophyceae) species from the China Sea based on morphological, molecular, and pigment data

Biology | Updated：2023-05-20

- Characterization of Amphidinium (Amphidiniales, Dinophyceae) species from the China Sea based on morphological, molecular, and pigment data
- Characterization of Amphidinium (Amphidiniales, Dinophyceae) species from the China Sea based on morphological, molecular, and pigment data
- 海洋湖沼学报（英文） 2022年40卷第3期页码：1191-1219
- Affiliations：
  
  1.Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China
  2.Research Center of Harmful Algae and Marine Biology, College of Life Science and Technology, Jinan University, Guangzhou 510362, China
  3.Shenzhen Academy of Environmental Science, Shenzhen 510008, China
  4.Botany & Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo 11751, Egypt
  5.Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai 519000, China
  6.Observation and Research Station of Coastal Wetland Ecosystem in Beibu Gulf, Ministry of Natural Resources, Beihai 536015, China
- Author bio：
  
  Songhui LÜ, lusonghui1963@163.com
  Haifeng GU, guhaifeng@tio.org.cn
- Funds：
  
  the Scientific Research Foundation of Third Institute of Oceanography, MNR(2017023);the National Natural Science Foundation of China(41806154);the National Natural Science Foundation of China(41876173);the National Natural Science Foundation of China(42076144);the Science and Technology Basic Resources Investigation Program of China(2018FY100200);the Special Foundation for National Science and Technology Basic Research Program of China(2018FY100100);the Project of Southern Marine Science and Engineering Guangdong Laboratory(311021004);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-021-1049-2
  中图分类号：
- 纸质出版：2022-03
- Accepted：
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Characterization of Amphidinium (Amphidiniales, Dinophyceae) species from the China Sea based on morphological, molecular, and pigment data[J]. 海洋湖沼学报（英文）, 2022,40(3):1191-1219.

Zhaohe LUO, Hua ZHANG, Qun LI, et al. Characterization of Amphidinium (Amphidiniales, Dinophyceae) species from the China Sea based on morphological, molecular, and pigment data[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1191-1219.
Characterization of Amphidinium (Amphidiniales, Dinophyceae) species from the China Sea based on morphological, molecular, and pigment data[J]. 海洋湖沼学报（英文）, 2022,40(3):1191-1219. DOI： 10.1007/s00343-021-1049-2.

Zhaohe LUO, Hua ZHANG, Qun LI, et al. Characterization of Amphidinium (Amphidiniales, Dinophyceae) species from the China Sea based on morphological, molecular, and pigment data[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1191-1219. DOI： 10.1007/s00343-021-1049-2.

摘要

Abstract

Amphidinium

species are amongst the most abundant benthic dinoflagellates in marine intertidal sandy ecosystems. Some of them are able to produce a variety of bioactive compounds that can have both harmful effects and pharmaceutical potentials. The diversity of

Amphidinium

in shallow waters along the Chinese coast was investigated by isolating single cells from sand

coral

and macroalgal samples collected from 2012 to 2020. Their morphologies were subjected to examination using light microscopy (LM) and scanning electron microscopy (SEM). A total of 74

Amphidinium

strains were morphologically identified

belonging to 11 species:

A. carterae

A. gibbosum

A. operculatum

A. massartii

cf.

massartii

A. fijiensis

A. pseudomassartii

A. steinii

A. thermaeum

A. theodori

A. tomasii

as well as an undefined species. The last seven species have not been previously reported in Chinese waters.

Amphidinium carterae

subclades I

and IV were found in the South China Sea

while subclade III was only found in the Yellow Sea. Threadlike body scales were observed on the surface of subclades III and V

supporting the idea that

A. carterae

might contain several different species. Large subunit ribosomal RNA (LSU rRNA) sequences-based phylogeny revealed two groups (Groups I and II) within

Amphidinium

which is consistent with the relative position of sulcus (in touch with cingulum or not). In addition

large differences in morphology and molecular phylogeny between

A. operculatum

(the type species of

Amphidinium

) and other species

suggest that a subdivision of

Amphidinium

might be needed. The pigment profiles of all available strains were analyzed by high performance liquid chromatography (HPLC). Eleven pigments

including peridinin

diadinoxanthin

diatoxanthin

pheophorbide (and pheophorbide a)

antheraxanthin

-carotene

and four different chlorophylls were detected. The high pheophorbide/pheophorbide

ratio in

Amphidinium

implies that it may be a good candidate as a natural source of photosensitizers

a well-known anticancer drug.

关键词

Keywords

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Characterization of Amphidinium (Amphidiniales, Dinophyceae) species from the China Sea based on morphological, molecular, and pigment data

DOI：10.1007/s00343-021-1049-2

摘要

Abstract

关键词

Keywords

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