Bacterial community composition in gut content and ambient sediment of two tropical wild sea cucumbers (<italic style="font-style: italic">Holothuria atra</italic> and <italic style="font-style: italic">H. leucospilota</italic>)

Fei GAO; Yue ZHANG; Peilin WU; Mengling CHEN; Linwen HE; Qiang XU; Aimin WANG

doi:10.1007/s00343-021-1001-5

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Bacterial community composition in gut content and ambient sediment of two tropical wild sea cucumbers (Holothuria atra and H. leucospilota)

Aquaculture and Fisheries | Updated：2023-04-27

- Bacterial community composition in gut content and ambient sediment of two tropical wild sea cucumbers (Holothuria atra and H. leucospilota)
- Bacterial community composition in gut content and ambient sediment of two tropical wild sea cucumbers (Holothuria atra and H. leucospilota)
- 海洋湖沼学报（英文） 2022年40卷第1期页码：360-372
- Affiliations：
  
  1.State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China
  2.Ocean College, Hainan University, Haikou 570228, China
- Author bio：
  
  Qiang XU, E-mail: xuqianghnu@hainanu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(41766005);the National Natural Science Foundation of China(31760757);the National Key Research and Development Program of China(2019YFD0901304);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-021-1001-5
  中图分类号：
- 收稿：2021-01-03，
  
  录用：2021-1-14，
  
  网络首发：2021-02-02，
  
  纸质出版：2022-01
- Accepted：
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Bacterial community composition in gut content and ambient sediment of two tropical wild sea cucumbers (Holothuria atra and H. leucospilota)[J]. 海洋湖沼学报（英文）, 2022,40(1):360-372.

Fei GAO, Yue ZHANG, Peilin WU, et al. Bacterial community composition in gut content and ambient sediment of two tropical wild sea cucumbers (Holothuria atra and H. leucospilota)[J]. Journal of Oceanology and Limnology, 2022, 40(1): 360-372.
Bacterial community composition in gut content and ambient sediment of two tropical wild sea cucumbers (Holothuria atra and H. leucospilota)[J]. 海洋湖沼学报（英文）, 2022,40(1):360-372. DOI： 10.1007/s00343-021-1001-5.

Fei GAO, Yue ZHANG, Peilin WU, et al. Bacterial community composition in gut content and ambient sediment of two tropical wild sea cucumbers (Holothuria atra and H. leucospilota)[J]. Journal of Oceanology and Limnology, 2022, 40(1): 360-372. DOI： 10.1007/s00343-021-1001-5.

摘要

Abstract

Sea cucumbers play an important role in sediment bioturbation in coral reef and rocky intertidal ecosystems

and bacteria were a commonly-reported component of holothuroid diets. Bacterial community composition in the foregut and hindgut of two common tropical sea cucumbers (

Holothuria atra

and

H. leucospilota

) and the ambient surface sediment were investigated using high throughput sequencing based on 16S rRNA gene analysis. A total of 5 584 operational taxonomic units (OTUs) were obtained from 25 samples based on a 97% threshold

and more than half of the OTUs (

=3 694

66.2%) were shared by the gut contents of two species of sea cucumbers and surrounding sediments. Bacterial richness and diversity in sediment samples were significantly higher than those in the gut content samples (

0.05). Proteobacteria was the predominant phylum in most samples showing 45.69%±8.61%

70.09%±4.03%

45.88%±5.38%

and 55.19%±0.79% reads in the foregut of

H. leucospilota

hindgut of

H. leucospilota

hindgut of

H. atra

and sediment libraries

respectively

but Bacteroidetes was the predominant phylum with the relative content of 34.98%±5.52% in the foregut of

H. atra

. Among the dominant genera

reads related to the genera

Anderseniella

Ilumatobacter

and

Ruegeria

were detected in all the gut contents and sediment libraries. A comparison of gut bacteria community between the two species of sea cucumbers suggested that

H. atra

had stronger feeding preference than

H. leucospilota

and the same types of microbes escaped digestion of the two sea cucumber species. Obvious different bacterial community composition in the foreguts of the two species of sea cucumbers and the surrounding sediments might result from the animal's selective feeding for sediment patches. Bacterial community structure in hindgut contents of

H. atra

and

H. leucospilota

both differed clearly from adjacent sediments

which indicated feeding activity of deposit-feeding sea cucumbers could change the sedimental bacterial composition. In conclusion

from the perspective of bacteria

sea cucumber

H. atra

and

H. leucospilota

had different feeding preferences

yet they could both affect bacterial composition in sediments by feeding activity. The motivation for selective feeding and sea cucumber-sediment interaction might be explored in the future.

关键词

Keywords

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DOI：10.1007/s00343-021-1001-5

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