Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in <italic style="font-style: italic">Macrobrachium rosenbergii</italic>

Zhili DING; Dongsheng ZHOU; Jinxian ZHENG; Xuefeng CHEN; Youqin KONG; Changle QI; Yan LIU; Qiongying TANG; Guoliang YANG; Jinyun YE

doi:10.1007/s00343-021-0494-2

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Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in Macrobrachium rosenbergii

Aquaculture and Fisheries | Updated：2023-05-20

- Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in Macrobrachium rosenbergii
- Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in Macrobrachium rosenbergii
- 海洋湖沼学报（英文） 2022年40卷第2期页码：805-817
- Affiliations：
  
  1.Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Science, Huzhou University, Huzhou 313000, China
  2.Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China
- Author bio：
  
  Zhili DING, E-mail: dingzhili@zjhu.edu.cn
- Funds：
  
  the Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture and the Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province of the Zhejiang Institute of Freshwater Fisheries(ZJK201906);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-021-0494-2
  中图分类号：
- 收稿：2021-01-09，
  
  录用：2021-3-30，
  
  网络首发：2021-05-11，
  
  纸质出版：2022-03
- Accepted：
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Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in Macrobrachium rosenbergii[J]. 海洋湖沼学报（英文）, 2022,40(2):805-817.

Zhili DING, Dongsheng ZHOU, Jinxian ZHENG, et al. Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in Macrobrachium rosenbergii[J]. Journal of Oceanology and Limnology, 2022, 40(2): 805-817.
Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in Macrobrachium rosenbergii[J]. 海洋湖沼学报（英文）, 2022,40(2):805-817. DOI： 10.1007/s00343-021-0494-2.

Zhili DING, Dongsheng ZHOU, Jinxian ZHENG, et al. Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in Macrobrachium rosenbergii[J]. Journal of Oceanology and Limnology, 2022, 40(2): 805-817. DOI： 10.1007/s00343-021-0494-2.

摘要

Abstract

Using alternative plant-derived dietary protein to replace fishmeal

combined with practical evaluation indexes

is a recent focus for aquaculture practices. An 8-week feeding experiment with giant freshwater prawn

Macrobrachium rosenbergii

post-larvae was conducted to determine the effects of replacing fishmeal (FM) with soybean meal in the feed

in terms of growth performance

antioxidant capacity

intestinal microbiota

and mRNA expression of target of rapamycin (TOR) and ribosomal protein S6 kinase B1 (S6K1). Four isonitrogenous diets with isocaloric value were prepared to contain 100%

75%

50%

or 25% FM as the protein source (dietary treatments FM100

FM75

FM50

and FM25

respectively). Each diet was fed to post-larval prawns (mean weight 0.045±0.002 g) twice a day in four replicates. No significant difference in weight gain was observed among all groups

but the survival rate of prawns fed the FM50 and FM25 diets was significantly lower than that of prawns fed the FM diet. The mRNA expression of both TOR and S6K1 were the lowest in hepatopancreas of prawns fed the FM25 diet. Superoxide dismutase activity of prawns fed the FM25 diet was significantly lower than that of prawns fed FM50. In contrast

the malondialdehyde content was significantly higher in prawns fed FM25 as compared with those fed FM75. The proportion of fishmeal in the diet did not affect the composition of core (phylum-level) intestinal microbiota

but greater fishmeal replacement with soybean meal had a potential risk to increase the relative abundance of opportunistic pathogens in the gut when considered at the genus level. These results suggest that fishmeal replacement with soybean meal should not exceed 50% in a diet for post-larval

M. rosenbergii

关键词

Keywords

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Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in Macrobrachium rosenbergii

Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in Macrobrachium rosenbergii

DOI：10.1007/s00343-021-0494-2

摘要

Abstract

关键词

Keywords

references