Diversity and seasonal variation of marine phytoplankton in Jiaozhou Bay, China revealed by morphological observation and metabarcoding

Tiantian Chen; Yingxin Zhang; Shuqun Song; Yun Liu; Xiaoxia Sun; Caiwen Li

doi:10.1007/s00343-021-0457-7

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Diversity and seasonal variation of marine phytoplankton in Jiaozhou Bay, China revealed by morphological observation and metabarcoding

Article | Updated：2023-05-20

- Diversity and seasonal variation of marine phytoplankton in Jiaozhou Bay, China revealed by morphological observation and metabarcoding
- Diversity and seasonal variation of marine phytoplankton in Jiaozhou Bay, China revealed by morphological observation and metabarcoding
- 海洋湖沼学报（英文） 2022年40卷第2期页码：577-591
- Affiliations：
  
  1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China
  3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
  4.University of Chinese Academy of Sciences, Beijing 100049, China
  5.Jiaozhou Bay National Marine Ecosystem Research Station, Chinese Academy of Sciences, Qingdao 266071, China
- Author bio：
  
  Caiwen LI, E-mail: cwli@qdio.ac.cn
- Funds：
  
  the National Science Foundation of China (NSFC)(41876120);the National Science Foundation of China (NSFC)(41906122);the National Science Foundation of China (NSFC)(41606128);the National Science Foundation of China (NSFC)(U1706218);the Science and Technology Basic Resources Investigation Program of China(2018FY100200);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-021-0457-7
  中图分类号：
- 收稿：2020-11-26，
  
  录用：2021-2-4，
  
  网络首发：2021-03-18，
  
  纸质出版：2022-03
- Accepted：
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Diversity and seasonal variation of marine phytoplankton in Jiaozhou Bay, China revealed by morphological observation and metabarcoding[J]. 海洋湖沼学报（英文）, 2022,40(2):577-591.

Tiantian Chen, Yingxin Zhang, Shuqun Song, et al. Diversity and seasonal variation of marine phytoplankton in Jiaozhou Bay, China revealed by morphological observation and metabarcoding[J]. Journal of Oceanology and Limnology, 2022, 40(2): 577-591.
Diversity and seasonal variation of marine phytoplankton in Jiaozhou Bay, China revealed by morphological observation and metabarcoding[J]. 海洋湖沼学报（英文）, 2022,40(2):577-591. DOI： 10.1007/s00343-021-0457-7.

Tiantian Chen, Yingxin Zhang, Shuqun Song, et al. Diversity and seasonal variation of marine phytoplankton in Jiaozhou Bay, China revealed by morphological observation and metabarcoding[J]. Journal of Oceanology and Limnology, 2022, 40(2): 577-591. DOI： 10.1007/s00343-021-0457-7.

摘要

Abstract

Phytoplankton are central components of marine environments

and are major players in the production and respiration budgeting. However

their diversity and distribution patterns are still poorly understood due largely to their small sizes and inconspicuous morphology that have been determined via the application of traditional morphology methods over the past two decades. To better understand the composition and diversity of phytoplankton in Jiaozhou Bay

China

seasonal sampling was carried out in 2019 and samples were analyzed with morphological observations and high-throughput sequencing

from which obvious seasonal variations in phytoplankton composition and proportional abundances were uncovered. Metabarcoding revealed far more diversity and species richness of phytoplankton than morphological observations

especially with respect to dinoflagellates. Diatoms were the most dominant phytoplankton group throughout the year

of which

Thalassionema

and

Skeletonema

were co-dominant in the bay. Parasitic dinoflagellates (e.g.

Amoebophrya

)

which is often overlooked in the morphological observations

were in dominance and high diversity in the metabarcoding dataset

thus more attention should be paid to exploring the potential role of parasitic dinoflagellates. Temperature

chlorophyll

and nutrient levels were the main influential factors on the distribution of phytoplankton. This study provided a comprehensive morphological and molecular description of phytoplankton and clearly demonstrated the importance of molecular technology in exploring phytoplankton communities. More-widespread use of molecular technology will facilitate deeper understanding of the ecological importance of the different species.

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references

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