Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ<sup>15</sup>N) analysis of otoliths

Huaiyu Bai; Yukun Wang; Tingting Zhang; Fangqun Dai; Lingfeng Huang; Yao Sun

doi:10.1007/s00343-021-0388-3

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Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ¹⁵N) analysis of otoliths

Article | Updated：2023-05-20

- Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ¹⁵N) analysis of otoliths
- Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ¹⁵N) analysis of otoliths
- 海洋湖沼学报（英文） 2022年40卷第2期页码：634-642
- Affiliations：
  
  1.College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
  2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
  3.Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen 361005, China
  4.Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071, China
- Author bio：
  
  Yao SUN, E-mail: sunyao@ysfri.ac.cn
- Funds：
  
  the National Key Basic Research Development Program of China (973 Program)(2015CB453303);the National Science Foundation for Young Scientists of China(41606195);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022
- DOI：10.1007/s00343-021-0388-3
  中图分类号：
- 收稿：2020-10-12，
  
  录用：2020-12-28，
  
  网络首发：2021-04-01，
  
  纸质出版：2022-03
- Accepted：
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Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ¹⁵N) analysis of otoliths[J]. 海洋湖沼学报（英文）, 2022,40(2):634-642.

Huaiyu Bai, Yukun Wang, Tingting Zhang, et al. Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ¹⁵N) analysis of otoliths[J]. Journal of Oceanology and Limnology, 2022, 40(2): 634-642.
Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ¹⁵N) analysis of otoliths[J]. 海洋湖沼学报（英文）, 2022,40(2):634-642. DOI： 10.1007/s00343-021-0388-3.

Huaiyu Bai, Yukun Wang, Tingting Zhang, et al. Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ¹⁵N) analysis of otoliths[J]. Journal of Oceanology and Limnology, 2022, 40(2): 634-642. DOI： 10.1007/s00343-021-0388-3.

摘要

Abstract

Fish otolith δ

N (δ

oto

) is a demonstrated source of information of dietary history for marine fish as it is available in otolith archives and sedimentary deposits unlike white muscle tissue (WMT). WMT and stomach content data are insufficient for trophic level (TL) data of past fishes which is important for the changes of marine fishery resources over long time scales. To determine the correlation between δ

oto

and fish WMT δ

N (δ

wmt

) and the feasibility of using δ

oto

in characterizing the TLs of marine fishes

we conducted nitrogen stable isotope analysis (SIA) in the otolith and WMT of 36 marine fish species sampled from the Yellow Sea and northern East China Sea in 2011–2014. Both δ

oto

and δ

wmt

were analyzed using an elemental analyzer coupled with an isotope ratio mass spectrometer (EA-IRMS). Multiple otoliths were combined to make each otolith measurement and were analyzed as-is without a carbonate dissolution pre-processing step. δ

oto

and δ

wmt

comparisons for species in the Yellow Sea and northern East China Sea are currently lacking and would be helpful for both regional studies and for increasing the number of species for which δ

oto

and δ

wmt

have been compared. Additionally

to determine the relative accuracy of trophic level calculated using δ

oto

we compared TL calculated from δ

oto

to traditional trophic level metrics calculated using δ

wmt

. The results showed a positive and highly significant correlation (

=0.780

0.001) between δ

oto

and δ

wmt

. Trophic level estimation using WMT (TL

wmt

) and otolith (TL

oto

) showed congruence in our study

which is not entirely surprising given that δ

oto

was regressed against δ

wmt

and the resulting regression coefficient was used to convert δ

oto

to δ

wmt

prior to calculating TL

oto

. This conversion was required in order to be consistent with previous δ

wmt

-based calculations of TL for comparison. TL

oto

calculations resulted in TL values that were largely within 5%–10% of TL values calculated with δ

wmt

. Our findings show that δ

oto

is a feasible technique for characterizing the TLs of marine fish and can also assist in food web and marine ecosystem studies.

关键词

Keywords

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Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ15N) analysis of otoliths

DOI：10.1007/s00343-021-0388-3

摘要

Abstract

关键词

Keywords

references

Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ¹⁵N) analysis of otoliths

Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ¹⁵N) analysis of otoliths