Expression and function of WNT4 involved in larvae development and limb regeneration in <italic style="font-style: italic">Portunus trituberculatus</italic>

Zhiming REN; Yuanyuan FU; Lei LIU; Xiao LIU; Chunlin WANG

doi:10.1007/s00343-020-9291-6

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Expression and function of WNT4 involved in larvae development and limb regeneration in Portunus trituberculatus

Biology | Updated：2023-05-20

- Expression and function of WNT4 involved in larvae development and limb regeneration in Portunus trituberculatus
- Expression and function of WNT4 involved in larvae development and limb regeneration in Portunus trituberculatus
- 海洋湖沼学报（英文） 2021年39卷第1期页码：306-316
- Affiliations：
  
  1.Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China
  2.Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315832, China
- Author bio：
  
  LIU Lei, E-mail:liulei1@nbu.edu.cn
  WANG Chunlin, E-mail:wangchunlin@nbu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(31602152);the Major Agriculture Program of Ningbo(2017C110007);the K. C. Wong Magana Fund in Ningbo University;The funding body had no role in the study design, experimental implementation, interpretation of data, or writing of the manuscript;© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021Abbreviation: CXCR4: C-X-C motif chemokine receptor 4; SHH: sonic hedgehog; WNT: wingless-related integration site; BCC: basal cell carcinoma; WNT4: wingless-type MMTV integration site family member-4; WNT3a: wingless-type MMTV integration site family member-3a; WNT5a: wingless-type MMTV integration site family member-5a; WNT7a: wingless-type MMTV integration site family member-7a; GFP: green fluorescent protein
- DOI：10.1007/s00343-020-9291-6
  中图分类号：
- 收稿：2019-12-12，
  
  录用：2020-4-13，
  
  网络首发：2020-05-05，
  
  纸质出版：2021-01
- Accepted：
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Expression and function of WNT4 involved in larvae development and limb regeneration in Portunus trituberculatus[J]. 海洋湖沼学报（英文）, 2021,39(1):306-316.

Zhiming REN, Yuanyuan FU, Lei LIU, et al. Expression and function of WNT4 involved in larvae development and limb regeneration in Portunus trituberculatus[J]. Journal of Oceanology and Limnology, 2021, 39(1): 306-316.
Expression and function of WNT4 involved in larvae development and limb regeneration in Portunus trituberculatus[J]. 海洋湖沼学报（英文）, 2021,39(1):306-316. DOI： 10.1007/s00343-020-9291-6.

Zhiming REN, Yuanyuan FU, Lei LIU, et al. Expression and function of WNT4 involved in larvae development and limb regeneration in Portunus trituberculatus[J]. Journal of Oceanology and Limnology, 2021, 39(1): 306-316. DOI： 10.1007/s00343-020-9291-6.

摘要

Abstract

The wingless-related integration site (WNT) proteins are a family of secreted glycoproteins that are evolutionarily conserved and are believed to be involved in evolution in vertebrates and invertebrates. WNT signaling pathways may be associated with limb regeneration and development in crustaceans. However

the detail mechanisms remain unclear. Therefore

the distribution of WNT4 in the hepatopancreas

muscle

hemocyte

ganglion

heart

eyestalk

gill tissue

and different larvae development stages of the swimming crab (

Portunus trituberculatus

) were characterized using immunofluorescence

real-time PCR

and Western blotting. Significant

PtWNT4

expression was detected in heart and eyestalk. In addition

PtWNT4

was expressed in all larval stages of

P. trituberculatus

with a dynamic expression pattern

especially in the eyestalk and other organs in the carapace area. The injection of

WNT4

dsRNA into regenerative limbs significantly decreased

PtWNT4

mRNA levels in the eyestalk

heart

and muscle

resulting in 1.9-fold

2.2-fold

and 2.7-fold decreases compared with those detected in the group injected with crab saline (

0.05)

respectively

indicating successful gene silencing. Overall

expression analysis on the WNT4 using RNAi provides an insight to its functional mechanism during limb regeneration in

P. trituberculatus

. The results not only demonstrated the requirement for WNT4 in limb regeneration of Crustaceans

but also suggested its ability to promote larval development at specific stages.

关键词

Keywords

references

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Expression and function of WNT4 involved in larvae development and limb regeneration in Portunus trituberculatus

Expression and function of WNT4 involved in larvae development and limb regeneration in Portunus trituberculatus

DOI：10.1007/s00343-020-9291-6

摘要

Abstract

关键词

Keywords

references