Complementary DNA sequencing (cDNA): an effective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients

Pingping HUANG; Feng ZHAO; Kuidong XU

doi:10.1007/s00343-020-9234-2

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Complementary DNA sequencing (cDNA): an effective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients

Ecology | Updated：2023-05-20

- Complementary DNA sequencing (cDNA): an effective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients
- Complementary DNA sequencing (cDNA): an effective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients
- 海洋湖沼学报（英文） 2021年39卷第1期页码：208-222
- Affiliations：
  
  1.Laboratory of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
  4.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
- Author bio：
  
  Kuidong XU, E-mail:kxu@qdio.ac.cn
- Funds：
  
  the National Natural Science Foundation of China(41876171);the National Natural Science Foundation of China(41506167);the National Natural Science Foundation of China(41476144);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021
- DOI：10.1007/s00343-020-9234-2
  中图分类号：
- 收稿：2019-09-19，
  
  录用：2019-11-18，
  
  网络首发：2020-01-28，
  
  纸质出版：2021-01
- Accepted：
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Complementary DNA sequencing (cDNA): an effective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients[J]. 海洋湖沼学报（英文）, 2021,39(1):208-222.

Pingping HUANG, Feng ZHAO, Kuidong XU. Complementary DNA sequencing (cDNA): an effective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients[J]. Journal of Oceanology and Limnology, 2021, 39(1): 208-222.
Complementary DNA sequencing (cDNA): an effective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients[J]. 海洋湖沼学报（英文）, 2021,39(1):208-222. DOI： 10.1007/s00343-020-9234-2.

Pingping HUANG, Feng ZHAO, Kuidong XU. Complementary DNA sequencing (cDNA): an effective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients[J]. Journal of Oceanology and Limnology, 2021, 39(1): 208-222. DOI： 10.1007/s00343-020-9234-2.

摘要

Abstract

The Yellow Sea Cold Water Mass (YSCWM) is a distinct hydrographic phenomenon of the Yellow Sea

and the distribution pattern of meio- and macrobenthos differs inside and outside of the YSCWM. However

such a pattern has never been observed in the microbenthic ciliate communities. Therefore

we hypothesized that benthic ciliates followed a similar distribution pattern as meio- and macrobenthos

but this pattern has not been uncovered by morphological methods. We evaluated the diversity and distribution of benthic ciliates at five stations along hydrographic gradients across the YSCWM and adjacent shallow water by using morphology and DNA and complementary DNA (cDNA) high-throughput sequencing of the V4 region of 18S rRNA gene. Results showed that the diversity of benthic ciliates detected by DNA (303 OTUs)

and the cDNA (611 OTUs) sequencing was much higher than that detected by the morphological method (79 species). Morphological method detected roughly different ciliate communities inside and outside of the YSCWM

but without statistical significance. No clear pattern was obtained by DNA sequencing. In contrast

cDNA sequencing revealed a distinct distribution pattern of benthic ciliate communities like meioand macrobenthos

which coincided well with the results of the environmental parameter analysis. More than half of the total sequences detected by DNA sequencing belonged to planktonic ciliates

most (if not all) of which were recovered from historic DNA originating through the sedimentation of pelagic forms because none of them were observed morphologically. The irrelevant historic DNA greatly influenced the recovery of rare species and thus limited the understanding of the benthic ciliate diversity and distribution. Our research indicates that the methods used have significant effects on the investigation of benthic ciliate communities and highlights that cDNA sequencing has great advantages in estimating the diversity and distribution of benthic ciliates

as well as the potential for benthic environmental assessments.

关键词

Keywords

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