Karyological observations of <italic style="font-style: italic">Ulva linza</italic> chromosomes

Xiaohui ZHAO; Xiaoqian YANG; Jianheng ZHANG; Qinlin WEN; Peimin HE

doi:10.1007/s00343-020-9105-x

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Karyological observations of Ulva linza chromosomes

Biology | Updated：2023-05-20

- Karyological observations of Ulva linza chromosomes
- Karyological observations of Ulva linza chromosomes
- 海洋湖沼学报（英文） 2021年39卷第1期页码：259-265
- Affiliations：
  
  1.College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China
  2.Water Environment & Engineering Research Center of Shanghai Institution of Higher Education, Shanghai 201306, China
- Author bio：
  
  Jianheng ZHANG, E-mail:jh-zhang@shou.edu.cn
  Peimin HE, E-mail:pmhe@shou.edu.cn
- Funds：
  
  the National Key R&D Program of China(2018YFD0901500);the Shanghai Sailing Program(17YF1407900);the National Natural Science Foundation of China(41576163);the Ocean Public Welfare Scientific Research Project, China(201205010);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021
- DOI：10.1007/s00343-020-9105-x
  中图分类号：
- 收稿：2019-04-21，
  
  录用：2019-9-23，
  
  网络首发：2020-02-16，
  
  纸质出版：2021-01
- Accepted：
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Karyological observations of Ulva linza chromosomes[J]. 海洋湖沼学报（英文）, 2021,39(1):259-265.

Xiaohui ZHAO, Xiaoqian YANG, Jianheng ZHANG, et al. Karyological observations of Ulva linza chromosomes[J]. Journal of Oceanology and Limnology, 2021, 39(1): 259-265.
Karyological observations of Ulva linza chromosomes[J]. 海洋湖沼学报（英文）, 2021,39(1):259-265. DOI： 10.1007/s00343-020-9105-x.

Xiaohui ZHAO, Xiaoqian YANG, Jianheng ZHANG, et al. Karyological observations of Ulva linza chromosomes[J]. Journal of Oceanology and Limnology, 2021, 39(1): 259-265. DOI： 10.1007/s00343-020-9105-x.

摘要

Abstract

Ulva linza

is one of the species that causes green tides in the Yellow Sea

China. Due to the difficulties in chromosomal preparation

the large numbers of chromosomes

and their relatively small sizes

there have been no reported studies on

Ulva

macroalgae chromosomes. The karyotypes and chromosomes in

U. linza

were observed after a series of treatments. The chromosomes were pretreated with 0.1% colchicine for 12 h and then mixed with enzymes. The samples were dropped from 30 cm height onto glass slides

which spread out the surface coat. These pretreatments were the optimal chromosomes preparation treatments. The prepared chromosomes were stained with 4'

6-diamidino-2-phenylindole (DAPI)

which is a fluorescent probe sensitive and specific to DNA. The chromosome number in the haploid male and female gametophytes was

and was 2

=18 in the diploid sporophytes. The female gametophyte chromosomes were between 0.804 and 2.292 μm in size

the male gametophyte chromosomes were between 0.917 and 2.916 μm

and the sporophyte chromosomes were between 0.912 and 2.167 μm. The relative sizes of the chromosomes were used to analyze the karyotypes of the female and male gametophyte chromosomes. The results provide a solid foundation for the basic technique that can be used to localize molecular markers of

Ulva

chromosomes.

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Keywords

references

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Karyological observations of Ulva linza chromosomes

Karyological observations of Ulva linza chromosomes

DOI：10.1007/s00343-020-9105-x

摘要

Abstract

关键词

Keywords

references