Ciliate <italic style="font-style: italic">Uronema marinum</italic> is the causative agent of scuticociliatosis in farm raised turbot <italic style="font-style: italic">Scophthalmus maximus</italic>

Guangxun DU; Lingyun QU; Kun SHANG; Chengjun SUN; Chen WANG; Ping GAO

doi:10.1007/s00343-020-8305-8

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Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus

Aquaculture and Fisheries | Updated：2023-05-20

- Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus
- Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus
- 海洋湖沼学报（英文） 2019年37卷第5期页码：1726-1735
- Affiliations：
  
  1.First Institute of Oceanography, Ministry of Natural Resources(MNR), Qingdao 266061, China
  2.Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
  3.College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
- Author bio：
  
  QU Lingyun, qly@fio.org.cn
- Funds：
  
  the National Key R & D Program of China(2017YFC1404504);the National Basic Research Program of China (973 Program)(2015CB755904);the Aoshan Technology Innovation Program of Qingdao National Laboratory for Marine Science and Technology(2015ASKJ02);the Aoshan Program Supported by Qingdao National Laboratory for Marine Science and Technology(2016ASKJ14);the Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology(2016LMFS-B08)
- DOI：10.1007/s00343-020-8305-8
  中图分类号：
- 收稿：2018-10-18，
  
  录用：2018-12-20，
  
  网络首发：2019-01-15，
  
  纸质出版：2019-09
- Accepted：
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Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus[J]. 海洋湖沼学报（英文）, 2019,37(5):1726-1735.

Guangxun DU, Lingyun QU, Kun SHANG, et al. Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus[J]. Journal of Oceanology and Limnology, 2019, 37(5): 1726-1735.
Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus[J]. 海洋湖沼学报（英文）, 2019,37(5):1726-1735. DOI： 10.1007/s00343-020-8305-8.

Guangxun DU, Lingyun QU, Kun SHANG, et al. Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus[J]. Journal of Oceanology and Limnology, 2019, 37(5): 1726-1735. DOI： 10.1007/s00343-020-8305-8.

摘要

Abstract

To identify the pathogen that causes scuticociliatosis in farmed-raised turbot

Scophthalmus maximus

we isolated a ciliate from the brain tissue of an infected turbot and identified it as

Uronema marinum

based on morphological and molecular evidence. We then infected the turbots in artificial laboratory settings with pure cultured

U. marinum

. The infected turbots showed syndromes similar to those observed in naturally infected ones. Furthermore

microscopic examination and PCR detection confirmed the presence of the ciliate in the tissues of those laboratory-infected turbots. To our best knowledge

this is the first report of scuticociliatosis caused by

U. marinum

in farm-raised turbot

S. maximus

in China.

关键词

Keywords

references

Anderson S A, Hulston D A, McVeagh S M, Webb V L, Smith P J. 2009. In vitro culture and cryopreservation of Uronema marinum isolated from farmed New Zealand groper ( Polyprion oxygeneios ). Journal of Microbiological Methods, 79(1):62-66, doi:10.1016/j.mimet. 2009.07.022..

Cardoso P H M, de Carvalho Balian S, Matushima E R, De Pádua S B, Martins M L. 2017. First report of scuticociliatosis caused by Uronema sp. in ornamental reef fish imported into Brazil. Revista Brasileira de Parasitologia Veterinaria, 26(4):491-495, doi:10.1590/s1984-29612017031.

Cawthorn R J, Lynn D H, Despres B, MacMillan R, Maloney R, Loughlin M, Bayer R. 1996. Description of Anophryoides haemophila n. sp. (Scuticociliatida:Orchitophryidae), a pathogen of American lobsters Homarus americanus . Diseases of Aquatic Organisms, 24(2):143-148, doi:10.3354/dao024143..

Cheung P J, Nigrelli R F, Ruggieri G D. 1980. Studies on the morphology of Uronema marinum Dujardin (Ciliatea:Uronematidae) with a description of the histopathology of the infection in marine fishes. Journal of Fish Diseases, 3(4):295-303, doi:10.1111/j.1365-2761.1980.tb00400.x.

Corliss J O. 1979. The Ciliated Protozoa. Characterization, Classification and Guide to the Literature. 2 nd edn.Pergamon Press, Oxford, UK.

Dyková I, Tyml T, Kostka M, Pecková H. 2010. Strains of Uronema marinum (Scuticociliatia) co-isolated with amoebae of the genus Neoparamoeba . Diseases of Aquatic Organisms, 89(1):71-77, doi:10.3354/dao02168..

Edgar R C. 2004. MUSCLE:multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32(5):1 792-1 797, doi:10.1093/nar/gkh340.

Figueras A, Robledo D, Corvelo A, Hermida M, Pereiro P, Rubiolo J A, Gómez-Garrido J, Carreté L, Bello X, Gut M, Gut I G, Marcet-Houben M, Forn-Cuní G, Galán B, García J L, Abal-Fabeiro J L, Pardo B G, Taboada X, Fernández C, Vlasova A, Hermoso-Pulido A, Guigó R, Álvarez-Dios J A, Gómez-Tato A, Viñas A, Maside X, Gabaldón T, Novoa B, Bouza C, Alioto T, Martínez P. 2016. Whole genome sequencing of turbot ( Scophthalmus maximus ; Pleuronectiformes):a fish adapted to demersal life. DNA Research, 23(3):181-192, doi:10.1093/dnares/dsw007.

Harikrishnan R, Balasundaram C, Heo M S. 2010.Scuticociliatosis and its recent prophylactic measures in aquaculture with special reference to South Korea:taxonomy, diversity and diagnosis of scuticociliatosis:part Ⅰ Control strategies of scuticociliatosis:part Ⅱ. Fish & Shellfish Immunology, 29(1):15-31, doi:10.1016/j.fsi.2010.02.026.

Iglesias R, Paramá A, Alvarez M F, Leiro J, Fernández J, Sanmartín M L. 2001. Philasterides dicentrarchi (Ciliophora, Scuticociliatida) as the causative agent of scuticociliatosis in farmed turbot Scophthalmus maximus in Galicia (NW Spain). Diseases of Aquatic Organisms, 46(1):47-55, doi:10.3354/dao046047..

Jee B Y, Kim Y C, Park M S. 2001. Morphology and biology of parasite responsible for scuticociliatosis of cultured olive flounder Paralichthys olivaceus . Diseases of Aquatic Organisms, 47(1):49-55, doi:10.3354/dao047049.

Kim S M, Cho J B, Kim S K, Nam Y K, Kim K H. 2004.Occurrence of scuticociliatosis in olive flounder Paralichthys olivaceus by Phiasterides dicentrarchi (Ciliophora:Scuticociliatida). Diseases of Aquatic Organisms, 62(3):233-238, doi:10.3354/dao062233..

Medlin L, Elwood H J, Stickel S, Sogin M L. 1988. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene, 71(2):491-499, doi:10.1016/0378-1119(88)90066-2.

Ofelio C, Blanco A, Roura Á, Pintado J, Pascual S, Planas M. 2013. Isolation and molecular identification of the scuticociliate Porpostoma notata Moebius, 1888 from moribund reared Hippocampus hippocampus (L.)seahorses, by amplification of the SSU rRNA gene sequences. Journal of Fish Diseases, 37(12):1 061-1 065, doi:10.1111/jfd.12207..

Piazzon M C, Leiro J, Lamas J. 2013. Fish immunity to scuticociliate parasites. Developmental & Comparative Immunology, 41(2):248-256, doi:10.1016/j.dci.2013.05.022.

Qin L, Wang Y G, Zhang L J, Dai, J X. 2007. Histopathology of turbot associated with Mesanophrys carcini parasite.Acta Hydrobiologica Sinica, 31(5):618-628. (in Chinese with English abstract)

Ramos M F, Costa A R, Barandela T, Saraiva A, Rodrigues P N. 2007. Scuticociliate infection and pathology in cultured turbot Scophthalmus maximus from the north of Portugal.Diseases of Aquatic Organisms, 74(3):249-253, doi:10.3354/dao074249.

Song W B, Warren A, Hu X Z. 2009. Free-living Ciliates in the Bohai and Yellow Seas, China. Science Press, Beijing, China. p.171-175. (in Chinese)

Stidworthy M F, Garner M M, Bradway D S, Westfall B D, Joseph B, Repetto S, Guglielmi E, Schmidt-Posthaus H, Thornton S M. 2014. Systemic Scuticociliatosis( Philasterides dicentrarchi ) in sharks. Veterinary Pathology, 51(3):628-632, doi:10.1177/0300985813492800.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013.MEGA6:molecular evolutionary genetics analysis Version 6.0. Molecular Biology and Evolution, 30(12):2 725-2 729, doi:10.1093/molbev/mst197.

Thompson Jr J C, Moewus L. 1964. Miamiensis avidus n. g., n.sp., a marine facultative parasite in the ciliate order Hymenostomatida. Journal of Eukaryotic Microbiology, 11(3):378-381, doi:10.1111/j.1550-7408.1964.tb01766.x.

Turgay E, Steinum T M, Gül A E, Karataş S. 2015. An outbreak of scuticociliatosis in cultured common dentex ( Dentex dentex ) in turkey. Bulletin-European Association of Fish Pathologists, 35(3):104-111.

Wang Y G, Chen J J, Qin L. 2005. Mesanphrys carcini causing severe scuticociliatosis in farmed turbot Scophthalmus maximus in China. Journal of Fishery Sciences of China, 12(5):594-601. (in Chinese with English abstract).

Whang I, Kang H S, Lee J. 2013. Identification of scuticociliates( Pseudocohnilembus persalinus , P. longisetus , Uronema marinum and Miamiensis avidus ) based on the cox1 sequence. Parasitology International, 62(1):7-13, doi:10.1016/j.parint.2012.08.002..

Wilbert N. 1975. Eine verbesserte Technik der Protargolimprägnation für Ciliaten. Mikrokosmos, 64:171-179.

Zhang Z, Wang Y G, Qin L, Li Q F. 2006. Epizootic investigation and study on challenge experiment of fin rot disease in cultured turbot ( Scophthalmus maximus ).Fisheries Science, 25(6):271-274. (in Chinese with English abstract)

Zhou L, Zhan W B, Song W B, Song C H. 2000. Observations on the histopathology of the external ulcerative disease of Japanese flounder, Paralichthys olivaceus . Journal of Ocean University of Qingdao, 30(4):593-597. (in Chinese with English abstract)

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Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus

Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus

DOI：10.1007/s00343-020-8305-8

摘要

Abstract

关键词

Keywords

references