Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns in<italic style="font-style: italic">Pyropia yezoensis</italic>(Bangiales, Rhodophyta)

Lu WANG; Junhao WANG; Yunke ZHU; Zhengcai CUI; Fanna KONG; Xianghai TANG; Yunxiang MAO

doi:10.1007/s00343-020-0298-9

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Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns inPyropia yezoensis(Bangiales, Rhodophyta)

Biology | Updated：2023-05-20

- Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns inPyropia yezoensis(Bangiales, Rhodophyta)
- Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns inPyropia yezoensis(Bangiales, Rhodophyta)
- 海洋湖沼学报（英文） 2021年39卷第4期页码：1447-1457
- Affiliations：
  
  1.Key Laboratory of Marine Genetics and Breeding(Ministry of Education), College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
  2.Key Laboratory of Utilization and Conservation of Tropical Marine Bioresource(Ministry of Education), College of Fisheries and Life Science, Hainan Tropical Ocean University, Sanya 572022, China
- Author bio：
  
  Xianghai TANG, E-mail:txianghai@ouc.edu.cn
  Yunxiang MAO, E-mail:yxmao@hntou.edu.cn
- Funds：
  
  the National Key R & D Program of China(2018YFD0900106);the National Key R & D Program of China(2018YFC1406700);the Marine S & T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)(2018SDKJ0302-4);the MOA Modern Agricultural Talents Support Project;© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021
- DOI：10.1007/s00343-020-0298-9
  中图分类号：
- 收稿：2020-08-02，
  
  录用：2020-8-26，
  
  网络首发：2020-08-27，
  
  纸质出版：2021-07
- Accepted：
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Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns inPyropia yezoensis(Bangiales, Rhodophyta)[J]. 海洋湖沼学报（英文）, 2021,39(4):1447-1457.

Lu WANG, Junhao WANG, Yunke ZHU, et al. Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns inPyropia yezoensis(Bangiales, Rhodophyta)[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1447-1457.
Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns inPyropia yezoensis(Bangiales, Rhodophyta)[J]. 海洋湖沼学报（英文）, 2021,39(4):1447-1457. DOI： 10.1007/s00343-020-0298-9.

Lu WANG, Junhao WANG, Yunke ZHU, et al. Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns inPyropia yezoensis(Bangiales, Rhodophyta)[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1447-1457. DOI： 10.1007/s00343-020-0298-9.

摘要

Abstract

The genus

Pyropia

contains several important cultivated species. Genetic research in nori species has mainly focused on the cell nucleus

with few studies on organelles (chloroplast and mitochondria). Due to the high copy numbers of organelles in cells

which influence the development and traits of algae

it is necessary to study their genetic mechanism. In this study

the marine red alga

Pyropia yezoensis

an important economic macroalga

was selected as the study object. To investigate organelle (chloroplast and mitochondria) inheritance in

P. yezoensis

the wild type RZ (maternal strain) was crossed with the red mutant HT (paternal strain) and 30 color-sectors from 11 F1 gametophytic blades were examined. The complete chloroplast and mitochondrial genomes of the red mutant (HT) were assembled for the first time. One reliable and stable single nucleotide polymorphism (SNP) loci filtrated by bioinformatics analysis was used as a molecular marker for chloroplast and mitochondrial DNA

respectively

in subsequent experiments. PCR amplification and sequence analysis showed that the haplotypes of color-sectors detected were consistent with those of the maternal parent

confirming that both chloroplast and mitochondrial genomes were inherited maternally in

P. yezoensis

. The inheritance pattern of organelles in

P. yezoensis

can be used to guide the hybridization and breeding of nori. Additionally

the organelle SNP markers developed in this study can be applied in subsequent genetic research.

关键词

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references

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Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns inPyropia yezoensis(Bangiales, Rhodophyta)

DOI：10.1007/s00343-020-0298-9

摘要

Abstract

关键词

Keywords

references