Metabolomics analysis for skin ulceration syndrome of<italic style="font-style: italic">Apostichopus japonicus</italic>based on UPLC/Q-TOF MS

Yang ZHANG; Youquan WANG; Xiaoyu LIU; Beichen DING; Yi SUN; Yaqing CHANG; Jun DING

doi:10.1007/s00343-020-0205-4

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Metabolomics analysis for skin ulceration syndrome ofApostichopus japonicusbased on UPLC/Q-TOF MS

Aquaculture and Fisheries | Updated：2023-05-20

- Metabolomics analysis for skin ulceration syndrome ofApostichopus japonicusbased on UPLC/Q-TOF MS
- Metabolomics analysis for skin ulceration syndrome ofApostichopus japonicusbased on UPLC/Q-TOF MS
- 海洋湖沼学报（英文） 2021年39卷第4期页码：1559-1569
- Affiliations：
  
  Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China
- Author bio：
  
  Jun DING, E-mail:dingjun3146@hotmail.com
- Funds：
  
  the National Natural Science Foundation of China(31772849);the Scientific Research Funding Project of Liaoning Provincial Department of Education in 2019(DL201901);the Program for Liaoning Innovative Research Team in University(LT2019003);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021
- DOI：10.1007/s00343-020-0205-4
  中图分类号：
- 收稿：2020-05-25，
  
  录用：2020-7-22，
  
  网络首发：2020-09-03，
  
  纸质出版：2021-07
- Accepted：
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Metabolomics analysis for skin ulceration syndrome ofApostichopus japonicusbased on UPLC/Q-TOF MS[J]. 海洋湖沼学报（英文）, 2021,39(4):1559-1569.

Yang ZHANG, Youquan WANG, Xiaoyu LIU, et al. Metabolomics analysis for skin ulceration syndrome ofApostichopus japonicusbased on UPLC/Q-TOF MS[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1559-1569.
Metabolomics analysis for skin ulceration syndrome ofApostichopus japonicusbased on UPLC/Q-TOF MS[J]. 海洋湖沼学报（英文）, 2021,39(4):1559-1569. DOI： 10.1007/s00343-020-0205-4.

Yang ZHANG, Youquan WANG, Xiaoyu LIU, et al. Metabolomics analysis for skin ulceration syndrome ofApostichopus japonicusbased on UPLC/Q-TOF MS[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1559-1569. DOI： 10.1007/s00343-020-0205-4.

摘要

Abstract

Skin ulceration syndrome (SUS) is the main diseases affected the development of sea cucumber (

Apostichopus japonicus

) culture industries. To better observe the changes in the sea cucumber

A. japonicus

with SUS and understand the pathogenesis of the disease

activities of superoxide dismutase (SOD)

catalase (CAT)

and level of malondialdehyde (MDA) in coelomic fluid were detected using the Assay Kit and metabolites in the body wall were assessed using ultra-performance liquid chromatography and quadrupole-time of flight mass spectrometry. The results indicated that level of MDA was increased during SUS compared with healthy individuals (

0.01)

but activities of SOD and CAT were reduced (

0.05). In metabolomics analysis

metabolites

such as adenosine

choline

betaine aldehyde

palmitic acid

and taurine

were found to be upregulated and 2-oxoadipic acid

anthranilic acid (vitamin L1)

thioetheramide-PC

cholesterol-3-sulfate

and pentadecanoic acid were downregulated (VIP

1 and

0.1). Pathway enrichment analysis indicated most enrichment of KEGG pathways were mainly related to energy metabolism

immunity

and osmoregulation such as ABC transporters

glycine

serine and threonine metabolism

tryptophan metabolism and neuroactive ligand-receptor interaction. Our study reflected the difference in enzyme activity and metabolites between

A. japonicus

with SUS and those without

which will provide reference data for investigating SUS.

关键词

Keywords

references

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Metabolomics analysis for skin ulceration syndrome ofApostichopus japonicusbased on UPLC/Q-TOF MS

Metabolomics analysis for skin ulceration syndrome ofApostichopus japonicusbased on UPLC/Q-TOF MS

DOI：10.1007/s00343-020-0205-4

摘要

Abstract

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