Species-specific detection and quantification of scyphomedusae in Jiaozhou Bay, China, using a quantitative real-time PCR assay

Jianyan WANG; Tiezhu MI; Zhigang YU; Guoshan WANG; Qinsheng WEI; Jing YANG; Yu ZHEN

doi:10.1007/s00343-020-0160-0

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Species-specific detection and quantification of scyphomedusae in Jiaozhou Bay, China, using a quantitative real-time PCR assay

Ecology | Updated：2023-05-20

- Species-specific detection and quantification of scyphomedusae in Jiaozhou Bay, China, using a quantitative real-time PCR assay
- Species-specific detection and quantification of scyphomedusae in Jiaozhou Bay, China, using a quantitative real-time PCR assay
- 海洋湖沼学报（英文） 2021年39卷第4期页码：1360-1372
- Affiliations：
  
  1.Department of Science Research, Beijing Museum of Natural History, Beijing 100050, China
  2.Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
  3.Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China
  4.College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China
  5.Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education/Institute for Advanced Ocean Study, Ocean University of China, Qingdao 266100, China
  6.National Marine Hazard Mitigation Service, Ministry of Natural Resources, Beijing 100194, China
  7.First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China
- Author bio：
  
  Yu ZHEN, E-mail:zhenyu@ouc.edu.cn
- Funds：
  
  the National Key Research and Development Program of China(2017YFC1404404);the National Key R & D Program of China(2017YFC1404402);the National Natural Science Foundation of China(41906112);the Scientific and Technological Innovation Project of the Qingdao National Laboratory for Marine Science and Technology(2016ASKJ02);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021
- DOI：10.1007/s00343-020-0160-0
  中图分类号：
- 收稿：2020-04-15，
  
  录用：2020-6-15，
  
  网络首发：2020-08-20，
  
  纸质出版：2021-07
- Accepted：
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Species-specific detection and quantification of scyphomedusae in Jiaozhou Bay, China, using a quantitative real-time PCR assay[J]. 海洋湖沼学报（英文）, 2021,39(4):1360-1372.

Jianyan WANG, Tiezhu MI, Zhigang YU, et al. Species-specific detection and quantification of scyphomedusae in Jiaozhou Bay, China, using a quantitative real-time PCR assay[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1360-1372.
Species-specific detection and quantification of scyphomedusae in Jiaozhou Bay, China, using a quantitative real-time PCR assay[J]. 海洋湖沼学报（英文）, 2021,39(4):1360-1372. DOI： 10.1007/s00343-020-0160-0.

Jianyan WANG, Tiezhu MI, Zhigang YU, et al. Species-specific detection and quantification of scyphomedusae in Jiaozhou Bay, China, using a quantitative real-time PCR assay[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1360-1372. DOI： 10.1007/s00343-020-0160-0.

摘要

Abstract

Over the past decades

jellyfish occurred increasingly and abundantly in coastal areas worldwide. Usually

biomass of jellyfish

especially when they bloom

can be determined by visual counting. However

tiny individuals of jellyfish (e.g.

planulae

polyps

and ephyrae) are difficult to detect in the field. In this study

species-specific quantitative real-time PCR assays (qPCR) (SYBR Green I) targeting the mitochondrial 16S rDNA (mt-16S rDNA) of jellyfish were developed and were used to estimate the distribution and seasonal fluctuations of four jellyfish species (

Nemopilema nomurai

Cyanea nozakii

Rhopilema esculentum

and

Aurelia coerulea

) in Jiaozhou Bay (JZB)

China in 2013. The mt-16S rDNA of

A. coerulea

and

N. nomurai

was detected in most of the survey months and it peaked in July (1.03×10

copies/L) and September (1.08×10

copies/L)

respectively. The mt-16S rDNA of

C. nozakii

occurred from August to October only with monthly mean values of 7.18-46.17 copies/L and was mainly located from the middle part to the outer part of the bay. The mt-16S rDNA of

R. esculentum

was the least abundant among the four species and was detected in only one sample (B2 station in March)

with a value of 88.49 copies/L. The Spearman correlation test revealed that phytoplankton biomass was significantly and positively correlated with the mt-16S rDNA abundance of

A. coerulea

(

=0.37

0.01) and negatively with the mt-16S rDNA of

N. nomurai

(

=-0.36

0.01). The qPCR assay will enable the identification and quantification of jellyfish species in their whole life history and can be used as an approach in combination of the traditional jellyfish survey.

关键词

Keywords

references

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