Isolation of a novel strain of <italic style="font-style: italic">Cyanobacterium</italic> sp. with good adaptation to extreme alkalinity and high polysaccharide yield

Zishuo CHEN; Tao LI; Bingjie YANG; Xuejie JIN; Hualian WU; Jiayi WU; Yandu LU; Wenzhou XIANG

doi:10.1007/s00343-020-0113-7

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Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield

Aquaculture and Fisheries | Updated：2023-05-20

- Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield
- Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield
- 海洋湖沼学报（英文） 2021年39卷第3期页码：1131-1142
- Affiliations：
  
  1.CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
  2.State Key Laboratory of Marine Resource Utilization in South China Sea, College of Oceanology, Hainan University, Haikou 570228, China
  3.Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou), Guangzhou 511458, China
  4.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  Yandu LU, E-mail: ydlu@hainu.edu.cn
  Wenzhou XIANG, E-mail: xwz@scsio.ac.cn
- Funds：
  
  Key-Area Research and Development Program of Guangdong Province(2020B1111030004);the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)(GML2019ZD0406);the 13th Five-Year Plan Marine Economy Innovation Development Demonstration Project(BHSFS004);the Project of State Key Laboratory of Marine Resource Utilization in South China Sea(2018004);the Guizhou Education Department Young scientific talents Promoting Program(KY[2016]160);the Project of Danzi (WetCode) Group(DZ201501);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021
- DOI：10.1007/s00343-020-0113-7
  中图分类号：
- 收稿：2020-03-05，
  
  录用：2020-4-22，
  
  网络首发：2020-06-08，
  
  纸质出版：2021-05
- Accepted：
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Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield[J]. 海洋湖沼学报（英文）, 2021,39(3):1131-1142.

Zishuo CHEN, Tao LI, Bingjie YANG, et al. Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield[J]. Journal of Oceanology and Limnology, 2021, 39(3): 1131-1142.
Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield[J]. 海洋湖沼学报（英文）, 2021,39(3):1131-1142. DOI： 10.1007/s00343-020-0113-7.

Zishuo CHEN, Tao LI, Bingjie YANG, et al. Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield[J]. Journal of Oceanology and Limnology, 2021, 39(3): 1131-1142. DOI： 10.1007/s00343-020-0113-7.

摘要

Abstract

The use of high alkaline medium is a feasible way to provide carbon source and prevent biological contamination for the outdoor cultivation of alkaliphilic microalgae and cyanobacteria. A novel cyanobacterial strain was isolated from the open pond of a marine green alga (

Picochlorum

sp. SCSIO-45015

Sanya

Hainan) and identified as

Cyanobacterium

sp. SCSIO-45682. The effects of initial sodium bicarbonate (NaHCO

) concentrations on the growth and biochemical composition of

Cyanobacterium

sp. SCSIO-45682 were investigated. The results demonstrated that

Cyanobacterium

sp. SCSIO-45682 had good adaptation to 16.8-g/L NaHCO

(the same concentration of NaHCO

used in Zarrouk medium for

Spirulina

). Moreover

the yields of biomass

polysaccharide

chlorophyll

(chl

)

and phycocyanin increased under high NaHCO

concentrations. The maximum final biomass concentration of 2.5 g/L was observed at 8.4-g/L NaHCO

while the highest intracellular total saccharide content of 49.2% of dry weight (DW) and exopolysaccharide (EPS) concentration of 93 mg/L were achieved at the NaHCO

concentration of 16.8 g/L. The crude protein content declined under high NaHCO

concentrations

which provide a possible explanation for the accumulation of polysaccharide. This study shows a good potential of alkaliphilic

Cyanobacterium

sp. SCSIO-45682 as a polysaccharide feedstock.

关键词

Keywords

references

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Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield

Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield

DOI：10.1007/s00343-020-0113-7

摘要

Abstract

关键词

Keywords

references