Digging out molecular markers associated with low salinity tolerance of <italic style="font-style: italic">Nannochloropsis oceanica</italic> through bulked mutant analysis

Sijie LIANG; Zhongyi ZHANG; Hang LIU; Li GUO; Shiyang SUN; Guanpin YANG

doi:10.1007/s00343-019-9189-3

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Digging out molecular markers associated with low salinity tolerance of Nannochloropsis oceanica through bulked mutant analysis

Biology | Updated：2023-04-27

- Digging out molecular markers associated with low salinity tolerance of Nannochloropsis oceanica through bulked mutant analysis
- Digging out molecular markers associated with low salinity tolerance of Nannochloropsis oceanica through bulked mutant analysis
- 海洋湖沼学报（英文） 2020年38卷第6期页码：1867-1879
- Affiliations：
  
  1.College of Marine Life Sciences, Ocean University of China(OUC), Qingdao 266003, China
  2.Key Laboratory of Marine Genetics and Breeding of Ministry of Education, OUC, Qingdao 266003, China
  3.College of Fisheries, OUC, Qingdao 266003, China
  4.Institute of Evolution and Marine Biodiversity, OUC, Qingdao 266003, China
- Author bio：
  
  GUO Li, qdguoli@139.com
  YANG Guanpin, yguanpin@ouc.edu.cn
- Funds：
  
  the National Key R&D Program of China(2018YFD0900305);the National Key R&D Program of China(2018YFD0901506);the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)(2018SDKJ0406-3);the Fundamental Research Funds for the Central Universities(201762017);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-9189-3
  中图分类号：
- 收稿：2019-07-22，
  
  录用：2019-9-9，
  
  网络首发：2019-10-16，
  
  纸质出版：2020-11
- Accepted：
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Digging out molecular markers associated with low salinity tolerance of Nannochloropsis oceanica through bulked mutant analysis[J]. 海洋湖沼学报（英文）, 2020,38(6):1867-1879.

Sijie LIANG, Zhongyi ZHANG, Hang LIU, et al. Digging out molecular markers associated with low salinity tolerance of Nannochloropsis oceanica through bulked mutant analysis[J]. Journal of Oceanology and Limnology, 2020, 38(6): 1867-1879.
Digging out molecular markers associated with low salinity tolerance of Nannochloropsis oceanica through bulked mutant analysis[J]. 海洋湖沼学报（英文）, 2020,38(6):1867-1879. DOI： 10.1007/s00343-019-9189-3.

Sijie LIANG, Zhongyi ZHANG, Hang LIU, et al. Digging out molecular markers associated with low salinity tolerance of Nannochloropsis oceanica through bulked mutant analysis[J]. Journal of Oceanology and Limnology, 2020, 38(6): 1867-1879. DOI： 10.1007/s00343-019-9189-3.

摘要

Abstract

Nannochloropsis oceanica

is a marine microalgal species with both economic value and biological importance. It grows fast

contains rich oils

reproduces asexually

holds a small and haploidy genome

and is easy to be modified genetically. However

the genetic study of

N. oceanica

is scarce. Very less genetic bases of its traits have been deciphered

and no gene has been isolated from it with the function verified simultaneously via either genetic or reverse genetic approaches or both (de novo cloned). Changing medium salinity may aid to control harmful organisms met during large scale cultivation. As a stress

it may also facilitate the accumulation of desirable chemicals including fatty acids. In order to decipher the genetic basis of the low salinity tolerance of

N. oceanica

we mutated

N. oceanica

with Zeocin. In total

five mutant bulks were constructed at equal number of cells

100 mutants each

which were tolerant to a discontinuous serial of salinities from that of 100% of f/2 to that of a mixture of 4% of f/2 and 94% of BG11. The bulks were genotyped through whole genome re-sequencing and analyzed with bulked mutant analysis (BMA) newly modified from bulked segregant analysis (BSA). In total

47 SNPs and 112 InDels were found to associate with the low salinity tolerance

and around them a set of low salinity tolerance associating genes were identified. A set of annotatable genes commonly found between control and different salinities indicated that the genes functioning in gene expression

energy metabolism and cellular structure may be involved in the low salinity tolerance. These associating molecular markers and genes around them were not enough for outlining the physiological mechanism underlining the tolerance; however they should aid to improve

N. oceanica

genetically.

关键词

Keywords

references

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Digging out molecular markers associated with low salinity tolerance of Nannochloropsis oceanica through bulked mutant analysis

Digging out molecular markers associated with low salinity tolerance of Nannochloropsis oceanica through bulked mutant analysis

DOI：10.1007/s00343-019-9189-3

摘要

Abstract

关键词

Keywords

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