Occurrence and light response of residual plastid genes in a <italic style="font-style: italic">Euglena gracilis</italic> bleached mutant strain OflB2

Huan QIN; Qingqing GUO; Chenchen LIU; Fenglan LI; Hua ZHANG; Zihan CHU; Jiangxin WANG; Anping LEI

doi:10.1007/s00343-019-9159-9

Your Location：

Home >

Browse articles >

Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2

Biology | Updated：2023-04-27

- Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2
- Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2
- 海洋湖沼学报（英文） 2020年38卷第6期页码：1858-1866
- Affiliations：
  
  1.Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518000, China
  2.Basis International School SZ, Shenzhen 518000, China
- Author bio：
  
  LEI Anping, bioaplei@szu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(31670116);the Guangdong Innovation Research Team Fund(2014ZT05S078);the Shenzhen Grant Plan for Science & Technology(JCYJ20160308095910917);the Shenzhen Grant Plan for Science & Technology(JCYJ20170818100339597);the Shenzhen Grant Plan for Science & Technology(JCYJ 20130329114940668);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-9159-9
  中图分类号：
- 收稿：2019-06-18，
  
  录用：2019-9-23，
  
  网络首发：2019-11-04，
  
  纸质出版：2020-11
- Accepted：
Scan QR Code
Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2[J]. 海洋湖沼学报（英文）, 2020,38(6):1858-1866.

Huan QIN, Qingqing GUO, Chenchen LIU, et al. Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2[J]. Journal of Oceanology and Limnology, 2020, 38(6): 1858-1866.
Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2[J]. 海洋湖沼学报（英文）, 2020,38(6):1858-1866. DOI： 10.1007/s00343-019-9159-9.

Huan QIN, Qingqing GUO, Chenchen LIU, et al. Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2[J]. Journal of Oceanology and Limnology, 2020, 38(6): 1858-1866. DOI： 10.1007/s00343-019-9159-9.

摘要

Abstract

Euglena gracilis

is a unicellular green eukaryotic microalga that features characteristics of both plants and animals. The photosynthetic function of its chloroplast is easily lost under stress resulting in bleached mutants

while the physiological role of their residual plastid DNAs remains unclear. In this study

we obtained five bleached mutants by ofloxacin (Ofl) treatment

identified 12 residual plastid genes in five bleached mutants

and determined the mRNA levels in the wild type

E. gracilis

(WT) and one bleached mutant (OflB2) under dark and light stimulation conditions by quantitative reverse transcribed PCR (qRTPCR). Results show that the expression of all selected plastid genes in both WT and OflB2 mutant did not change significantly in darkness

while their responses to light stimulation were different. Under the light stimulation conditions

half of the genes did not change significantly

while most of the other genes were down-regulated in OflB2 mutant and up-regulated in WT. Therefore

the bleached mutant retains part of the plastid genome and the plastid relic is responsive to light. Our research will help to understand the functions of residual plastid DNA and evolution of chloroplasts.

关键词

Keywords

references

N Ahmadinejad , T Dagan , W Martin . Genome history in the symbiotic hybrid Euglena gracilis . Gene. , 2007 . 402 ( 1-2 ): 35 - 39 . DOI: 10.1016/j.gene.2007.07.023 http://doi.org/10.1016/j.gene.2007.07.023 .

M S Bennett , R E Triemer . Chloroplast genome evolution in the Euglenaceae . J. Eukaryot. Microbiol. , 2015 . 62 ( 6 ): 773 - 785 . DOI: 10.1111/jeu.12235 http://doi.org/10.1111/jeu.12235 .

A Bodyl . Is the origin of Astasia longa an example of the inheritance of acquired characteristics? . Acta Protozool. , 1996 . 35 ( 2 ): 87 - 94 . .

Buetow D E. 1982. The Biology of Euglena , Vol. III. Academic Press, New York. p.157-195.

Ebenezer T E, Carrington M, Lebert M, Kelly S, Field M C. 2017. Euglena gracilis genome and transcriptome: organelles, nuclear genome assembly strategies and initial features. In : Schwartzbach S D, Shigeoka S eds. Euglena: Biochemistry, Cell and Molecular Biology. Springer, Cham. p.125-140.

S Eberhard , D Drapier , F A Wollman . Searching limiting steps in the expression of chloroplast-encoded proteins: relations between gene copy number, transcription, transcript abundance and translation rate in the chloroplast of Chlamydomonas reinhardtii . Plant J. , 2002 . 31 ( 2 ): 149 - 160 . DOI: 10.1046/j.1365-313X.2002.01340.x http://doi.org/10.1046/j.1365-313X.2002.01340.x .

G Gockel , W Hachtel , S Baier , C Fliss , M Henke . Genes for components of the chloroplast translational apparatus are conserved in the reduced 73-kb plastid DNA of the nonphotosynthetic euglenoid flagellate Astasia longa . Curr. Genet , 1994 . 26 ( 3 ): 256 - 262 . DOI: 10.1007/BF00309557 http://doi.org/10.1007/BF00309557 .

G Gockel , W Hachtel , M Michael . Complete gene map of the plastid genome of the nonphotosynthetic euglenoid flagellate Astasia longa . Protist , 2000 . 151 ( 4 ): 347 - 351 . DOI: 10.1078/S1434-4610(04)70033-4 http://doi.org/10.1078/S1434-4610(04)70033-4 .

L Hadariová , M Vesteg , E Birčák , S D Schwartzbach , J Krajčovič . An intact plastid genome is essential for the survival of colorless Euglena longa but not Euglena gracilis . Curr. Genet , 2017 . 63 ( 2 ): 331 - 341 . DOI: 10.1007/s00294-016-0641-z http://doi.org/10.1007/s00294-016-0641-z .

R B Hallick , L Hong , R G Drager , M R Favreau , A Monfort , B Orsat , A Spielmann , E Stutz . Complete sequence of Euglena gracilis chloroplast DNA . Nucleic Acids Res. , 1993 . 21 ( 15 ): 3 537 - 3 544 . DOI: 10.1093/nar/21.15.3537 http://doi.org/10.1093/nar/21.15.3537 .

P Heizmann , J Doly , Y Hussein , P Nicolas , V Nigon , G Bernardi . The chloroplast genome of bleached mutants of Euglena gracilis . Biochim. Biophys. Acta , 1981 . 653 ( 3 ): 412 - 415 . DOI: 10.1016/0005-2787(81)90197-0 http://doi.org/10.1016/0005-2787(81)90197-0 .

P Heizmann , P Ravel-Chapuis , V Nigon . Minicircular DNA having sequence homologies with chloroplast DNA in a bleached mutant of Euglena gracilis . Curr. Genet , 1982 . 6 ( 2 ): 119 - 122 . .

P Heizmann , G F Salvador , V Nigon . Occurrence of plastidial rRNAs and plastidial structures in bleached mutants of Euglena gracilis . Exp. Cell Res. , 1976 . 99 ( 2 ): 253 - 260 . DOI: 10.1016/0014-4827(76)90581-4 http://doi.org/10.1016/0014-4827(76)90581-4 .

Y Hussein , P Heizmann , P Nicolas , V Nigon . Quantitative estimations of chloroplast DNA in bleached mutants of Euglena gracilis . Curr. Genet , 1982 . 6 ( 2 ): 111 - 117 . .

A D Idoine , A Boulouis , J Rupprecht , R Bock . The diurnal logic of the expression of the chloroplast genome in Chlamydomonas reinhardtii . PLoS One , 2014 . 9 ( 10 ): e108760 DOI: 10.1371/journal.pone.0108760 http://doi.org/10.1371/journal.pone.0108760 .

P A Kivic , M Vesk . Structure and function in the euglenoid eyespot apparatus: the fine structure, and response to environmental changes . Planta , 1972 . 105 ( 1 ): 1 - 14 . DOI: 10.1007/BF00385158 http://doi.org/10.1007/BF00385158 .

P A Kivic , M Vesk . An electron microscope search for plastids in bleached Euglena gracilis and in Astasia longa . Can. J. Bot. , 1974 . 52 ( 4 ): 695 - 699 . DOI: 10.1139/b74-089 http://doi.org/10.1139/b74-089 .

J Krajčovič , L Ebringer , J Polónyi . Quinolones and coumarins eliminate chloroplasts from Euglena gracilis . Antimicrob. Agents Chemother. , 1989 . 33 ( 11 ): 1 883 - 1 889 . DOI: 10.1128/AAC.33.11.1883 http://doi.org/10.1128/AAC.33.11.1883 .

N I Krinsky , T H Goldsmith . The carotenoids of the flagellated alga, Euglena gracilis . Arch. Biochem. Biophys. , 1960 . 91 ( 2 ): 271 - 279 . DOI: 10.1016/0003-9861(60)90501-4 http://doi.org/10.1016/0003-9861(60)90501-4 .

L Križková , M Nagy , J Polónyi , L Ebringer . The effect of flavonoids on ofloxacin-induced mutagenicity in Euglena gracilis . Mutat. Res. , 1998 . 416 ( 1-2 ): 85 - 92 . DOI: 10.1016/S1383-5718(98)00080-1 http://doi.org/10.1016/S1383-5718(98)00080-1 .

K Krnáčová , I Rydlová , M Vinarčíková , J Krajčovič , M Vesteg , A Horváth . Characterization of oxidative phosphorylation enzymes in Euglena gracilis and its white mutant strain W gm ZOflL . FEBS Lett. , 2015 . 589 ( 6 ): 687 - 694 . DOI: 10.1016/j.febslet.2015.01.035 http://doi.org/10.1016/j.febslet.2015.01.035 .

D J Oldenburg , A J Bendich . The linear plastid chromosomes of maize: terminal sequences, structures, and implications for DNA replication . Curr. Genet , 2016 . 62 ( 2 ): 431 - 442 . .

H W Qin , L F Chen , N Lu , Y H Zhao , X Yuan . Toxic effects of enrofloxacin on Scenedesmus obliquus . Front. Environ. Sci. Eng. , 2012 . 6 ( 1 ): 107 - 116 . DOI: 10.1007/s11783-011-0327-1 http://doi.org/10.1007/s11783-011-0327-1 .

S D Schwartzbach , J A Schiff . Chloroplast and cytoplasmic ribosomes of Euglena : selective binding of dihydrostreptomycin to chloroplast ribosomes . J. Bacteriol. , 1974 . 120 ( 1 ): 334 - 341 . DOI: 10.1128/JB.120.1.334-341.1974 http://doi.org/10.1128/JB.120.1.334-341.1974 .

G Siemeister , W Hachtel . A circular 73 kb DNA from the colourless flagellate Astasia longa that resembles the chloroplast DNA of Euglena : restriction and gene map . Curr. Genet , 1989 . 15 ( 6 ): 435 - 441 . DOI: 10.1007/BF00376801 http://doi.org/10.1007/BF00376801 .

C Sulli , Z W Fang , U Muchhal , S D Schwartzbach . Topology of Euglena chloroplast protein precursors within endoplasmic reticulum to Golgi to chloroplast transport vesicles . J. Biol. Chem. , 1999 . 274 ( 1 ): 457 - 463 . DOI: 10.1074/jbc.274.1.457 http://doi.org/10.1074/jbc.274.1.457 .

E J Thomas , W Ortiz . Loss of chloroplast transcripts for proteins associated with photosystem II: an early event during heat-bleaching in Euglena gracilis . Plant Mol. Biol. , 1995 . 27 ( 2 ): 317 - 325 . DOI: 10.1007/BF00020186 http://doi.org/10.1007/BF00020186 .

S Tucci , R Vacula , J Krajčovič , P Proksch , W Martin . Variability of wax ester fermentation in natural and bleached Euglena gracilis strains in response to oxygen and the elongase inhibitor flufenacet . J. Eukaryot. Microbiol. , 2010 . 57 ( 1 ): 63 - 69 . DOI: 10.1111/j.1550-7408.2009.00452.x http://doi.org/10.1111/j.1550-7408.2009.00452.x .

R Vacula , J M Steiner , J Krajčovič , L Ebringer , W Löffelhardt . Plastid state- and light-dependent regulation of the expression of nucleus-encoded genes for chloroplast proteins in the flagellate Euglena gracilis . Folia Microbiol. , 2001 . 46 ( 5 ): 433 - 441 . DOI: 10.1007/BF02814435 http://doi.org/10.1007/BF02814435 .

M Vesteg , R Vacula , S Burey , W Löffelhardt , H Drahovská , W Martin , J Krajčovič . Expression of nucleus-encoded genes for chloroplast proteins in the flagellate Euglena gracilis . J. Eukaryot. Microbiol. , 2009 . 56 ( 2 ): 159 - 166 . DOI: 10.1111/j.1550-7408.2008.00383.x http://doi.org/10.1111/j.1550-7408.2008.00383.x .

J X Wang , Z X Shi , X D Xu . Chloroplast-less mutants of two species of Euglena . Acta Hydrobiol. Sin. , 2002 . 26 ( 2 ): 175 - 179 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ssswxb200202011 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ssswxb200202011 , .

J X Wang , Z X Shi , X D Xu . Residual plastids of bleached mutants of Euglena gracilis and their effects on the expression of nucleus-encoded genes . Prog. Nat. Sci. , 2004 . 14 ( 3 ): 213 - 217 . DOI: 10.1080/10020070412331343371 http://doi.org/10.1080/10020070412331343371 .

Y Yoshida , T Tomiyama , T Maruta , M Tomita , T Ishikawa , K Arakawa . De novo assembly and comparative transcriptome analysis of Euglena gracilis in response to anaerobic conditions . BMC Genomics , 2016 . 17 182 DOI: 10.1186/s12864-016-2540-6 http://doi.org/10.1186/s12864-016-2540-6 .

Zakryś B, Milanowski R, Karnkowska A. 2017. Evolutionary origin of Euglena . In : Schwartzbach S D, Shigeoka S eds. Euglena: Biochemistry, Cell and Molecular Biology. Springer, Cham. p.3-17.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据

Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2

Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2

DOI：10.1007/s00343-019-9159-9

摘要

Abstract

关键词

Keywords

references