Defensive physiological characters of <italic style="font-style: italic">Pyropia yezoensis</italic> resistant lines to the red rot disease

Dandan ZOU; Xianghai TANG; Liping QIU; Yunxiang MAO

doi:10.1007/s00343-019-9144-3

Your Location：

Home >

Browse articles >

Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease

Updated：2023-04-27

- Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease
- Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease
- 海洋湖沼学报（英文） 2020年38卷第2期页码：509-516
- Affiliations：
  
  1.College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
  2.Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China
- Author bio：
  
  MAO Yunxiang, ocean0629@korea.kr
- Funds：
  
  the National Natural Science Foundation of China(31372517);the National Natural Science Foundation of China(41006082);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-9144-3
  中图分类号：
- 收稿：2019-05-23，
  
  录用：2019-7-11，
  
  网络首发：2019-08-05，
  
  纸质出版：2020-03
- Accepted：
Scan QR Code
Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease[J]. 海洋湖沼学报（英文）, 2020,38(2):509-516.

Dandan ZOU, Xianghai TANG, Liping QIU, et al. Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease[J]. Journal of Oceanology and Limnology, 2020, 38(2): 509-516.
Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease[J]. 海洋湖沼学报（英文）, 2020,38(2):509-516. DOI： 10.1007/s00343-019-9144-3.

Dandan ZOU, Xianghai TANG, Liping QIU, et al. Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease[J]. Journal of Oceanology and Limnology, 2020, 38(2): 509-516. DOI： 10.1007/s00343-019-9144-3.

摘要

Abstract

To explore effective physiological indexes to distinguish the resistant lines and susceptible lines of

Pythium porphyrae

the causal agent of red rot disease of

Pyropia yezoensis

and establish the disease-resistance breeding strategy

we obtained and analyzed the candidate resistant and susceptible lines by population selection. Gametophytes of the candidate lines were cultured in seawater containing

Pyt. porphyrae

zoospores. Antioxidase activities

including superoxide dismutase (SOD)

peroxidase (POD) and polyphenol oxidase (PPO)

were measured and compared between the two lines before and after infection. In the resistant lines

SOD and POD activities increased and then decreased

but PPO activity rose with the prolongation of the infection time. The phenylalanine ammonia lyase (PAL) activities also increased and then decreased after infection

but it had significantly different expression in the two lines without pathogen attack. The synthesis rates of β-1

3-glucanase

and cell-wall degrading enzyme were different from each other between the two lines after infection of

Pyt. porphyrae

. Comparison in the contents of malondialdehyde (MDA) and reactive oxygen species (ROS) in the two lines showed that

the two contents varied synchronously in response to the pathogen attack. Changes of these enzymes activities or contents demonstrated that

Pyr. yezoensis

could resist against the pathogen of

Pyt. porphyrae

with the antioxidant defense capacity. In addition

β-1.3-glucanase content showed extremely significant difference between the two lines

and the PAL had consistent expression difference. Therefore

phenylalanine ammonia lyase (PAL) and β-1

3-glucanase can be considered as an effective index to distinguish susceptible line and resistant line

with which the workload of the resistant breeding could be reduced in the future.

关键词

Keywords

references

F B Abeles , R P Bosshart , L E Forrence . Preparation and purification of glucanase and chitinase from bean leaves . Plant Physiology , 1971 . 47 ( 1 ): 129 - 134 . DOI: 10.1104/pp.47.1.129 http://doi.org/10.1104/pp.47.1.129 .

P Albersheim , B S Valent . Host-pathogen interactions Ⅶ.Plant pathogens secrete proteins which inhibit enzymes of the host capable of attacking the pathogen . Plant Physiology , 1974 . 53 ( 5 ): 684 - 687 . DOI: 10.1104/pp.53.5.684 http://doi.org/10.1104/pp.53.5.684 .

Y Aoki , Y Kamei . Preparation of recombinant polysaccharide-degrading enzymes from the marine bacterium, Pseudomonas sp.ND137 for the production of protoplasts of Porphyra yezoensis . European Journal of Phycology , 2006 . 41 ( 3 ): 321 - 328 . DOI: 10.1080/09670260600801682 http://doi.org/10.1080/09670260600801682 .

I Apostol , P F Heinstein , P S Low . Rapid stimulation of an oxidative burst during elicitation of cultured plant cells role in defense and signal transduction . Plant physiology , 1989 . 90 ( 1 ): 109 - 116 . DOI: 10.1104/pp.90.1.109 http://doi.org/10.1104/pp.90.1.109 .

M Baucher , M A Bernard-Vailhé , B Chabbert , 等 . Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa ( Medicago sativa L . ) and the effect on lignin composition and digestibility. Plant Molecular Biology , 1999 . 39 ( 3 ): 437 - 447 . .

K Bouarab , F Adas , E Gaquerel , 等 . The innate immunity of a marine red alga involves oxylipins from both the eicosanoid and octadecanoid pathways . Plant Physiology , 2004 . 135 ( 3 ): 1838 - 1848 . DOI: 10.1104/pp.103.037622 http://doi.org/10.1104/pp.103.037622 .

G X Chen , K Asada . Ascorbate peroxidase in tea leaves:occurrence of two isozymes and the differences in their enzymatic and molecular properties . Plant and Cell Physiology , 1989 . 30 ( 7 ): 987 - 998 . .

J Collén , C Hervé , I Guisle-Marsollier , 等 . Expression profiling of Chondrus crispus (Rhodophyta) after exposure to methyl jasmonate . Journal of Experimental Botany , 2006 . 57 ( 14 ): 3869 - 3881 . DOI: 10.1093/jxb/erl171 http://doi.org/10.1093/jxb/erl171 .

C H Foyer , P Descourvieres , K J Kunert . Protection against oxygen radicals:an important defense mechanism studied in transgenic plants . Plant Cell and Environment , 1994 . 17 ( 5 ): 507 - 523 . DOI: 10.1111/j.1365-3040.1994.tb00146.x http://doi.org/10.1111/j.1365-3040.1994.tb00146.x .

J Friend , S B Reynolds , M A Aveyard . Phenylalanine ammonia lyase, chlorogenic acid and lignin in potato tuber tissue inoculated with Phytophthora infestans . Physiological Plant Pathology , 1973 . 3 ( 4 ): 495 - 507 . DOI: 10.1016/0048-4059(73)90060-X http://doi.org/10.1016/0048-4059(73)90060-X .

Y Kawamura , K Yokoo , M Tojo , 等 . Distribution of Pythium porphyrae , the causal agent of red rot disease of Porphyrae spp., in the Ariake Sea, Japan . Plant Disease , 2005 . 89 ( 10 ): 1041 - 1047 . .

H Koca , M Bor , F Özdemir , 等 . The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars . Environmental and Experimental Botany , 2007 . 60 ( 3 ): 344 - 351 . DOI: 10.1016/j.envexpbot.2006.12.005 http://doi.org/10.1016/j.envexpbot.2006.12.005 .

J Koukol , E E Conn . The metabolism of aromatic compounds in higher plants Ⅳ . Purification and properties of the phenylalanine deaminase ofHordeum vulgare. Journal of Biological Chemistry , 1961 . 236 ( 10 ): 2692 - 2698 . .

F C Küpper , B Kloareg , J Guern , 等 . Oligoguluronates elicit an oxidative burst in the brown algal kelp Laminaria digitata . Plant Physiology , 2001 . 125 ( 1 ): 278 - 291 . DOI: 10.1104/pp.125.1.278 http://doi.org/10.1104/pp.125.1.278 .

F L Li , Y Fu , Q Yuan , W H Lü , Y Q Xu , R M Liu , B Z Hu , F W Chen , Y Y Xu , Y Z Feng . Variations in antioxidase activities and MDA content in potato tubers infected by Fusarium trichothecioides . Agricultural Science and Technology , 2015 . 16 ( 11 ): 2433 - 2436 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hnnykjtx-e201511014 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hnnykjtx-e201511014 , .

Y Liang , Q Chen , Q Liu , 等 . Exogenous silicon (Si)increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley ( Hordeum vulgare L . ). Journal of Plant Physiology , 2003 . 160 ( 10 ): 1157 - 1164 . DOI: 10.1078/0176-1617-01065 http://doi.org/10.1078/0176-1617-01065 .

F Mauch , L A Hadwiger , T Boller . Ethylene:symptom, not signal for the induction of chitinase and β-1, 3-glucanase in pea pods by pathogens and elicitors . Plant Physiology , 1984 . 76 ( 3 ): 607 - 611 . DOI: 10.1104/pp.76.3.607 http://doi.org/10.1104/pp.76.3.607 .

R Mittler . Oxidative stress, antioxidants and stress tolerance . Trends in Plant Science , 2002 . 7 ( 9 ): 405 - 410 . DOI: 10.1016/S1360-1385(02)02312-9 http://doi.org/10.1016/S1360-1385(02)02312-9 .

C S Park , E K Hwang . Isolation and evaluation of a strain of Pyropia yezoensis (Bangiales, Rhodophyta)resistant to red rot disease . Journal of Applied Phycology , 2014 . 26 ( 2 ): 811 - 817 . DOI: 10.1007/s10811-013-0183-4 http://doi.org/10.1007/s10811-013-0183-4 .

C S Park , M Kakinuma , H Amano . Detection and quantitative analysis of zoospores of Pythium porphyrae , causative organism of red rot disease in Porphyra , by competitive PCR . Journal of Applied Phycology , 2001 . 13 433 - 441 . DOI: 10.1023/A:1011982105124 http://doi.org/10.1023/A:1011982105124 .

M Peng , J Kuc . Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf disks . Phytopathology , 1992 . 82 ( 6 ): 696 - 699 . DOI: 10.1094/Phyto-82-696 http://doi.org/10.1094/Phyto-82-696 .

H B Shao , S Y Jiang , F M Li , 等 . Some advances in plant stress physiology and their implications in the systems biology era . Colloids and Surfaces B:Biointerfaces , 2007 . 54 ( 1 ): 33 - 36 . DOI: 10.1016/j.colsurfb.2006.05.011 http://doi.org/10.1016/j.colsurfb.2006.05.011 .

F O Stefani , P Tanguay , G Pelletier , 等 . Impact of endochitinase-transformed white spruce on soil fungal biomass and ectendomycorrhizal symbiosis . Applied and Environmental Microbiology , 2010 . 76 ( 8 ): 2607 - 2614 . DOI: 10.1128/AEM.02807-09 http://doi.org/10.1128/AEM.02807-09 .

N Veitía , L R García , I Bermúdez-Caraballoso , 等 . Field resistance of selected potato clones to early blight . Biotecnología Vegetal , 2014 . 14 ( 4 ): 215 - 221 . .

F Weinberger , P Leonardi , A Miravalles , 等 . Dissection of two distinct defense-related responses to agar oligosaccharides in Gracilaria chilensis (Rhodophyta) and Gracilaria conferta (Rhodophyta) . Journal of Phycology , 2005 . 41 ( 4 ): 863 - 873 . DOI: 10.1111/j.0022-3646.2005.05009.x http://doi.org/10.1111/j.0022-3646.2005.05009.x .

S Werner , U Steiner , R Becher , 等 . Chitin synthesis during in planta growth and asexual propagation of the cellulosic oomycete and obligate biotrophic grapevine pathogen Plasmopara viticola . FEMS Microbiology Letters , 2002 . 208 ( 2 ): 169 - 173 . DOI: 10.1111/j.1574-6968.2002.tb11077.x http://doi.org/10.1111/j.1574-6968.2002.tb11077.x .

J H Woo , E Kitamura , H Myouga , 等 . An antifungal protein from the marine bacterium Streptomyces sp . strain AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp. Applied and Environmental Microbiology , 2002 . 68 ( 6 ): 2666 - 2675 . http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_123949 http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_123949 , .

C T Wu , K J Bradford . Class I chitinase and β-1, 3-glucanase are differentially regulated by wounding, methyl jasmonate, ethylene, and gibberellin in tomato seeds and leaves . Plant Physiology , 2003 . 133 ( 1 ): 263 - 273 . DOI: 10.1104/pp.103.024687 http://doi.org/10.1104/pp.103.024687 .

X N Zhang , P P Sun , Y X Mao , 等 . The variation of defensive compounds content and protective enzymes activity of Pyropia yezoensis gametophyte infected by oomycete Pythium porphyrae . Journal of Ocean University of China (Natural Science) , 2015 . 45 ( 12 ): 57 - 64 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=qdhydxxb201512009 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=qdhydxxb201512009 , .

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis

Characterization, functional properties, and antioxidant activities of macromolecular extracts isolated from Pyropia yezoensis

Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns inPyropia yezoensis(Bangiales, Rhodophyta)

Construction of high-density genetic linkage map of Pyropia yezoensis (Bangiales, Rhodophyta) and identification of red color trait QTLs in the thalli

Development of a PCR method for detection of Pseudoalteromonas marina associated with green spot disease in Pyropia yezoensis

Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease

Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease

DOI：10.1007/s00343-019-9144-3

摘要

Abstract

关键词

Keywords

references