Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells

Shengnan ZHOU; Ge LIU; Shimei WU

doi:10.1007/s00343-019-9129-2

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Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells

Updated：2023-05-19

- Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells
- Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells
- 海洋湖沼学报（英文） 2020年38卷第3期页码：826-833
- Affiliations：
  
  1.College of Life Sciences, Qingdao University, Qingdao 266071, China
  2.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  3.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
- Author bio：
  
  WU Shimei, shimeiwu2016@126.com
- Funds：
  
  the National Natural Science Foundation of China(31600035);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-9129-2
  中图分类号：
- 收稿：2019-05-15，
  
  录用：2019-8-19，
  
  网络首发：2019-08-26，
  
  纸质出版：2020-03
- Accepted：
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Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells[J]. 海洋湖沼学报（英文）, 2020,38(3):826-833.

Shengnan ZHOU, Ge LIU, Shimei WU. Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells[J]. Journal of Oceanology and Limnology, 2020, 38(3): 826-833.
Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells[J]. 海洋湖沼学报（英文）, 2020,38(3):826-833. DOI： 10.1007/s00343-019-9129-2.

Shengnan ZHOU, Ge LIU, Shimei WU. Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells[J]. Journal of Oceanology and Limnology, 2020, 38(3): 826-833. DOI： 10.1007/s00343-019-9129-2.

摘要

Abstract

Marine bacterial strain

Bacillus

sp. CS30 exhibited high anticancer activity against Huh7.5 human liver cancer. We purified the corresponding anticancer agent by sequential acidic precipitation

methanol extraction

Sephadex LH-20 chromatography

and reversed phase high-performance liquid chromatography (RP-HPLC)

then analyzed it in mass spectrometry. Based on the results of purification and mass spectrometry

we deduced that the anticancer agent was the same component as our previously purified antifungal agent surfactin CS30-2. However

to the best of our knowledge

this is the first report on the surfactin possessing both antifungal and anticancer activities. Surfactin CS30-2 was demonstrated to exhibit high anticancer activity in a dose-dependent manner against Huh7.5 liver cancer cells. Further investigation showed that surfactin CS30-2 induced the increased generation of reactive oxygen species (ROS) and severe disruption of cell membrane

thus leading to cell death. However

unlike previously reported surfactins

surfactin CS30-2 caused cancer cell death via necrosis instead of apoptosis.

关键词

Keywords

references

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